| 质粒名称: | pAAV-CAG-NTnC_166D_202D |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000189 |
| 产品用途: | Expresses the mutant of the NTnC_166D+_202D+ green calcium indicator based on mNeonGreen and troponin from O. tau toadfish with blocked sensitivity to calcium ions in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | NTnC_166D_202D |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites
Barykina N.V., Subach O.M., Doronin D.A., Sotskov V.P., Roshchina M.A., Kunitsyna T.A., Malyshev A.Y., Smirnov I.V., Azieva A.M., Sokolov I.S., Piatkevich K.D., Burtsev M.S., Varizhuk A.M., Pozmogova G.E.E., Anokhin K.V., Subach F.V., Enikolopov G.N.
Sci. Reports 2016 61 6, 1–15 (2016)
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买家导航
pAAV-CAG-NTnC_166D_202D载体质粒基本信息
pAAV-CAG-NTnC_166D_202D质粒图谱载体图谱和pAAV-CAG-NTnC_166D_202D载体序列质粒序列多克隆位点信息
pAAV-CAG-NTnC_166D_202D质粒载体简介
pAAV-CAG-NTnC_166D_202D质粒序列载体序列
LOCUS pAAV-CAG-NTnC_166D_202D 5683 bp DNA circular SYN 08-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5683)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..5683
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2911..3188
/label=chicken beta-actin promoter
regulatory 3619..3628
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3625..4566
/codon_start=1
/label=NTnC/166D+/202D+
/translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGSGNPNVGYE
ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVQFED
GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMANSLTAMVPSEEELSECFRTFDKD
GDEGFIDREEFGGIIRLTGEQLTDEDPDEIFGDSDTDKNEGRIDFDEFLKMVENVQLSM
ADWCRSKMACPNDKTLISTLKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVF
RKTELKHSKTEMDFKEWQKAFTDVMGMDELYK"
misc_feature 4585..5173
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5056..5067)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5216..5337
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ccattgacgt caataatgac gtatgttccc atagtaacgc
2701 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
2761 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
2821 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
2881 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
2941 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
3001 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
3061 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
3121 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
3181 ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
3241 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
3301 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
3361 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
3421 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
3481 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
3541 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattggg
3601 gatccggtac cggctagcgc caccatggtc tcaaagggag aggaggacaa catggctagt
3661 ctgcctgcta cccacgaact gcatattttt ggctccataa atggcgtcga tttcgacatg
3721 gtaggccaag gctccggtaa tccaaacgtc ggttatgagg agctgaacct gaagtccacc
3781 aagggcgatc tgcagttcag tccctggatc ctggtgccgc acattgggta cgggtttcat
3841 cagtatcttc cctacccgga cgggatgtca ccatttcagg ctgcaatggt cgacgggtcc
3901 ggatatcagg tgcaccgcac tgtgcagttt gaggacggtg cttcactgac cgtgaattac
3961 agatacacct acgaaggatc acatatcaaa ggggaagctc aggtgaaggg cactgggttc
4021 ccggcagacg gccctgtgat ggcaaattcc ctgacagcca tggtcccgag cgaagaagag
4081 ctatcagagt gctttcgcac ttttgacaag gatggagatg agggtttcat cgacagggag
4141 gaatttggag gcatcatccg ccttactgga gaacagctca cagatgagga tcccgatgag
4201 atatttggag attcagacac agacaaaaat gagggaagga ttgactttga tgagttcctg
4261 aagatggtgg agaatgtcca gttgtccatg gctgactggt gtagatccaa gatggcctgc
4321 cccaatgata agactctcat ttccacactt aagtggtctt ataccaccgg caacggcaaa
4381 cgctaccggt ctactgctcg caccacatac acattcgcca aaccaatggc cgcaaattac
4441 ctcaaaaatc agccgatgta tgtctttcgg aaaacagagc tgaaacattc taagaccgaa
4501 atggatttta aagagtggca aaaggccttt acggacgtga tgggcatgga cgagctgtat
4561 aagtaagaat tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
4621 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
4681 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
4741 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
4801 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
4861 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
4921 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
4981 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
5041 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
5101 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
5161 gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga
5221 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
5281 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
5341 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
5401 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta
5461 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
5521 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
5581 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga
5641 atcggccaac gcgcggggag aggcggtttg cgtattgggc gct
//
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