| 质粒名称: | Antares-SEPLuc |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 141086 |
| 存储实验室: | Jeak Ling Ding |
|
载体骨架基本信息 |
|
| 载体名称: | pCDNA3.1 |
| 载体出品公司: | Thermo Fisher Scientific |
| 空载体大小: | 4606bp |
| 完整质粒大小: | 9178 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Antares-T2A-puromycin-EMCV IRES-SEPLu |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 4572 bp |
| 基因突变: | Deletion of amino acids 66 to 91 on the Luciferase region (Please see depositor comments) |
| 启动子: | CMV |
| 克隆方法: | 未知 |
| 5’测序引物 | T7 forward (TTAATACGACTCACTATAGGG) |
| 3’测序引物 | BGH reverse (TAGAAGGCACAGTCGAGG) |
|
文献引用方法 |
|
| 材料和方法部分: | Antares-SEPLuc was a gift from Jeak Ling Ding (Addgene plasmid # 141086 ; http://n2t.net/addgene:141086 ; RRID:Addgene_141086) |
| 参考文献部分: | pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis. Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL. Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. 10.3389/fbioe.2020.00412 PubMed 32457886 |
买家导航
Antares-SEPLuc载体质粒基本信息
Antares-SEPLuc质粒图谱载体图谱和Antares-SEPLuc载体序列质粒序列多克隆位点信息
Antares-SEPLuc质粒载体简介
Antares-SEPLuc质粒序列载体序列
LOCUS Antares-SEPLuc 9092 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION precursor of the bioluminescent reporter pHluc, for detecting
extracellular pH changes in tumor acidosis.
ACCESSION .
VERSION .
KEYWORDS Antares-SEPLuc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9092)
AUTHORS Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
TITLE pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of
Tumor Acidosis.
JOURNAL Front Bioeng Biotechnol. 2020 May 8;8:412. doi:
10.3389/fbioe.2020.00412. eCollection 2020.
PUBMED 32457886
REFERENCE 2 (bases 1 to 9092)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9092
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(23..40)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 29..253
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 299..727
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(386..405)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 596..617
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(736..756)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 741..1070
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 921..1056
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 983..1002
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 1310..1431
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1347..1366)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1401..1420
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1480..1496)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1480..1496)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1493..1515)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1504..1520
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1528..1558)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1573..1594
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1711..1728)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1882..2470)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1962..1981)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2641..3501)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3264..3283
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3502..3606)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3681..3700)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3872..4251
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4252..4455
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4406..4426
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4500..4519
/label=T7
/note="T7 promoter, forward primer"
promoter 4500..4518
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 6411..6464
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6471..7070
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6471..6490)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6967..6987
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 7072..7658
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7239..7256)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7466..7485
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
ORIGIN
1 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
61 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
121 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
181 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
241 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
301 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
361 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
421 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
481 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
601 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
721 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
781 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
901 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
961 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
1021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
1081 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcgcgg
1141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
1201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
1261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
1321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
1381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
1441 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
1501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
1561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
1621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
1681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
1741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
1801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
1861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
1921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
1981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
2041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
2101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
2161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
2221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
2281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
2341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
2401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
2461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
2521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
2581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
2641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
2701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
2761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
2821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
2881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
2941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
3001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
3061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
3121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
3181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
3241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
3301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
3361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
3421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
3481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
3541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
3601 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
3661 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
3721 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
3781 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
3841 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
3901 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
3961 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
4021 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
4081 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
4141 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
4201 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
4261 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
4321 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
4381 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
4441 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
4501 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg
4561 agctcggatc cactagtcca gtgtggtgga attcatggtg agcaagggcg aggagctgat
4621 caaggagaac atgagaagca agctgtacct ggaaggcagc gtgaacggcc accagttcaa
4681 gtgcacccac gaaggggagg gcaagcccta cgagggcaag cagaccaaca ggatcaaggt
4741 ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacccact ttatgtacgg
4801 gagcaaggtg ttcatcaagt accccgccga cctccccgat tattttaagc agtccttccc
4861 tgagggcttc acatgggaga gagtcatggt gttcgaagac gggggcgtgc tgaccgccac
4921 ccaggacacc agcctccagg acggcgagct catctacaac gtcaaggtca gaggggtgaa
4981 cttcccagcc aacggccccg tgatgcagaa gaaaacactg ggctgggagc ccagcaccga
5041 gaccatgtac cccgctgacg gcggcctgga aggcagatgc gacaaggccc tgaagctcgt
5101 gggcgggggc cacctgcacg tcaacttcaa gaccacatac aagtccaaga aacccgtgaa
5161 gatgcccggc gtccactacg tggaccgcag actggaaaga atcaaggagg ccgacaacga
5221 gacctacgtc gagcagtacg agcacgctgt ggccagatac tccaacctgg gcggaggctt
5281 cacactcgaa gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt
5341 ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat
5401 ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc
5461 gtatgaagga ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
5521 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
5581 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
5641 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
5701 gcattctggc gaggcacgag ctgatcaagg agaacatgag aagcaagctg tacctggaag
5761 gcagcgtgaa cggccaccag ttcaagtgca cccacgaagg ggagggcaag ccctacgagg
5821 gcaagcagac caacaggatc aaggtggtgg agggaggccc cctgccgttc gcattcgaca
5881 tcctggccac ccactttatg tacgggagca aggtgttcat caagtacccc gccgacctcc
5941 ccgattattt taagcagtcc ttccctgagg gcttcacatg ggagagagtc atggtgttcg
6001 aagacggggg cgtgctgacc gccacccagg acaccagcct ccaggacggc gagctcatct
6061 acaacgtcaa ggtcagaggg gtgaacttcc cagccaacgg ccccgtgatg cagaagaaaa
6121 cactgggctg ggagcccagc accgagacca tgtaccccgc tgacggcggc ctggaaggca
6181 gatgcgacaa ggccctgaag ctcgtgggcg ggggccacct gcacgtcaac ttcaagacca
6241 catacaagtc caagaaaccc gtgaagatgc ccggcgtcca ctacgtggac cgcagactgg
6301 aaagaatcaa ggaggccgac aacgagacct acgtcgagca gtacgagcac gctgtggcca
6361 gatactccaa cctgggcgga ggcatggacg agctgtacaa ggcggccgcc gagggcagag
6421 gaagtcttct aacatgcggt gacgtggagg agaatcccgg cccttccggg atgaccgagt
6481 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
6541 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
6601 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
6661 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
6721 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
6781 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
6841 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
6901 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
6961 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
7021 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acccctctcc
7081 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
7141 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
7201 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
7261 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
7321 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
7381 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
7441 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
7501 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
7561 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
7621 tttcctttga aaaacacgat gataatatgg ccacaaccat ggttgagatg ttgccaaccg
7681 tagctgtgct cgttttggct gtcagcgtag tcgctaaaga taatacaaca cttcaggagt
7741 tcgcaacatc taagggagaa gagttgttta ctggcgtagt acccattctt gtggaacttg
7801 acggcgatgt taatggtcat aagttctctg tgtctggtga gggcgagggg gatgctacat
7861 atggtaaatt gaccctcaaa tttatttgta caaccgggaa gctccctgta ccctggccta
7921 ctctcgtgac caccctcacc tacggggtac aatgtttttc cagatacccc gatcacatga
7981 agcgccacga tttcttcaaa agtgccatgc ccgagggtta cgtccaggaa cgcacaatat
8041 ttttcaagga tgatggaaat tacaaaaccc gcgccgaggt taagttcgag ggggataccc
8101 tcgttaaccg gattgaactg aagggtatcg actttaagga ggacggtaat atattggggc
8161 ataaattgga atacaactat aacgaccacc aggtgtatat catggccgat aaacagaaga
8221 acggcatcaa agcaaacttt aaaattaggc ataacataga ggatggtggc gttcagcttg
8281 ctgatcacta tcaacagaat acccctatag gtgatggacc cgtgctcctc ccagataacc
8341 attacttgtt tacaacttct acactctcta aggaccccaa cgaaaagaga gaccacatgg
8401 tgctcttgga gttcgtaact gccgccggaa ttacacatgg tatggacgag ctctacaagg
8461 atatttccgg gggggtcttt acacttgaag atttcgtcgg ggattggaga caaactgccg
8521 gttataatct tgatcaggtg cttgagcaag ggggggtgag ctcccttttt caaaacctgg
8581 gtgtatctgt cacccctatc caaaggattg tgcttagcgg tgaaaatggt ctcaaaatcg
8641 acatccacgt gatcatccca tacgagggtc ttagtggaga tcagatgggg cagatagaga
8701 agattttcaa agttgtttac cctgtcgacg accaccattt caaagtaatt ctgcactacg
8761 gaaccctcgt catagacggg gtcacaccca acatgataga ttactttggg cgaccctatg
8821 aagggatagc agtattcgac ggcaaaaaga taaccgtgac aggcaccctg tggaatggaa
8881 acaaaattat agatgagcgc ctgatcaacc ctgacggttc tttgttgttc agggttacaa
8941 tcaatggtgt cacaggctgg aggctctgtg agcgcatcct ggctcagttg gagaatgggg
9001 ggatctccct cttggtgcaa aatacatcat ggatgttgct tcttcttctt agcttgagcc
9061 tcttgcaggc attggatttc atcagtctct aa
//
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