| 质粒名称: | pCDNA-3xHA-hTERT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51637 |
| 存储实验室: | Steven Artandi |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen/LIfe Technologies |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | N端子3xFLAG标记在Kozak之后 |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TERT |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3399 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | 3xHA（主干网上的N个终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AscI(未破坏) |
| 3' 克隆位点: | PacI(未破坏) |
| 5' 测序引物 | CMV/T7 |
| 3' 测序引物 | bGH reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCDNA-3xHA-hTERT was a gift from Steven Artandi (Addgene plasmid # 51637 ; http://n2t.net/addgene:51637 ; RRID:Addgene_51637) |
| 参考文献部分: | Identification of ATPases pontin and reptin as telomerase components essential for holoenzyme assembly. Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE. Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019. 10.1016/j.cell.2008.01.019 PubMed 18358808 |
买家导航
pCDNA-3xHA-hTERT载体质粒基本信息
pCDNA-3xHA-hTERT质粒图谱载体图谱和pCDNA-3xHA-hTERT载体序列质粒序列多克隆位点信息
pCDNA-3xHA-hTERT质粒载体简介
pCDNA-3xHA-hTERT质粒序列载体序列
LOCUS pCDNA-3xHA-hTERT 9027 bp ds-DNA circular SYN 12-NOV-2018
DEFINITION Mammalian expression of 3xHA tagged human TERT, N-terminal..
ACCESSION .
VERSION .
KEYWORDS pCDNA-3xHA-hTERT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9027)
AUTHORS Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
TITLE Identification of ATPases pontin and reptin as telomerase components
essential for holoenzyme assembly.
JOURNAL Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
PUBMED 18358808
REFERENCE 2 (bases 1 to 9027)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 12, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9027
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 249..268
/label=T7
/note="T7 promoter, forward primer"
promoter 249..267
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 346..355
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 367..393
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 397..423
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 427..453
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3917..3946
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3962..3979
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(4002..4019)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4008..4232
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4278..4706
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4365..4384)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4575..4596
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4715..4735)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4720..5049
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4900..5035
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4962..4981
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5116..5910
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5170..5189)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5780..5799
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6086..6207
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6123..6142)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6177..6196
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6256..6272)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6256..6272)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6269..6291)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6280..6296
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6304..6334)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6349..6370
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6487..6504)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6658..7246)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6738..6757)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7417..8277)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8040..8059
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8278..8382)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8457..8476)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8648..9027
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg
301 agctcggatc cactagtcca gtgtggtgga attctgcaga taaacgccac catggggcaa
361 caaggatacc cgtatgatgt tccggattac gctggctacc catacgacgt cccagactac
421 gctggctacc catacgacgt cccagactac gctcaaggag tgcggccgcg ggggcgcgcc
481 ccgcgcgctc cccgctgccg agccgtgcgc tccctgctgc gcagccacta ccgcgaggtg
541 ctgccgctgg ccacgttcgt gcggcgcctg gggccccagg gctggcggct ggtgcagcgc
601 ggggacccgg cggctttccg cgcgctggtg gcccagtgcc tggtgtgcgt gccctgggac
661 gcacggccgc cccccgccgc cccctccttc cgccaggtgt cctgcctgaa ggagctggtg
721 gcccgagtgc tgcagaggct gtgcgagcgc ggcgcgaaga acgtgctggc cttcggcttc
781 gcgctgctgg acggggcccg cgggggcccc cccgaggcct tcaccaccag cgtgcgcagc
841 tacctgccca acacggtgac cgacgcactg cgggggagcg gggcgtgggg gctgctgctg
901 cgccgcgtgg gcgacgacgt gctggttcac ctgctggcac gctgcgcgct ctttgtgctg
961 gtggctccca gctgcgccta ccaggtgtgc gggccgccgc tgtaccagct cggcgctgcc
1021 actcaggccc ggcccccgcc acacgctagt ggaccccgaa ggcgtctggg atgcgaacgg
1081 gcctggaacc atagcgtcag ggaggccggg gtccccctgg gcctgccagc cccgggtgcg
1141 aggaggcgcg ggggcagtgc cagccgaagt ctgccgttgc ccaagaggcc caggcgtggc
1201 gctgcccctg agccggagcg gacgcccgtt gggcaggggt cctgggccca cccgggcagg
1261 acgcgtggac cgagtgaccg tggtttctgt gtggtgtcac ctgccagacc cgccgaagaa
1321 gccacctctt tggatggtgc gctctctggc acgcgccact cccacccatc cgtgggccgc
1381 cagcaccacg cgggcccccc atccacatcg cggccaccac gtccctggga cacgccttgt
1441 cccccggtgt acgccgagac caagcacttc ctctactcct caggcgacaa ggagcagctg
1501 cggccctcct tcctactcag ctctctgagg cccagcctga ctggcgctcg gaggctcgtg
1561 gagaccatct ttctgggttc caggccctgg atgccaggga ctccccgcag gttgccccgc
1621 ctgccccagc gctactggca aatgcggccc ctgtttctgg agctgcttgg gaaccacgcg
1681 cagtgcccct acggggtgct cctcaagacg cactgcccgc tgcgagctgc ggtcacccca
1741 gcagccggtg tctgtgcccg ggagaagccc cagggctctg tggcggcccc cgaggaggag
1801 gacacagacc cccgtcgcct ggtgcagctg ctccgccagc acagcagccc ctggcaggtg
1861 tacggcttcg tgcgggcctg cctgcgccgg ctggtgcccc caggcctctg gggctccagg
1921 cacaacgaac gccgcttcct caggaacacc aagaagttca tctccctggg gaagcatgcc
1981 aagctctcgc tgcaggagct gacgtggaag atgagcgtgc gggactgcgc ttggctgcgc
2041 aggagcccag gggttggctg tgttccggcc gcagagcacc gtctgcgtga ggagatcctg
2101 gccaagttcc tgcactggct gatgagtgtg tacgtcgtcg agctgctcag gtctttcttt
2161 tatgtcacgg agaccacgtt tcaaaagaac aggctctttt tctaccggaa gagtgtctgg
2221 agcaagttgc aaagcattgg aatcagacag cacttgaaga gggtgcagct gcgggagctg
2281 tcggaagcag aggtcaggca gcatcgggaa gccaggcccg ccctgctgac gtccagactc
2341 cgcttcatcc ccaagcctga cgggctgcgg ccgattgtga acatggacta cgtcgtggga
2401 gccagaacgt tccgcagaga aaagagggcc gagcgtctca cctcgagggt gaaggcactg
2461 ttcagcgtgc tcaactacga gcgggcgcgg cgccccggcc tcctgggcgc ctctgtgctg
2521 ggcctggacg atatccacag ggcctggcgc accttcgtgc tgcgtgtgcg ggcccaggac
2581 ccgccgcctg agctgtactt tgtcaaggtg gatgtgacgg gcgcgtacga caccatcccc
2641 caggacaggc tcacggaggt catcgccagc atcatcaaac cccagaacac gtactgcgtg
2701 cgtcggtatg ccgtggtcca gaaggccgcc catgggcacg tccgcaaggc cttcaagagc
2761 cacgtctcta ccttgacaga cctccagccg tacatgcgac agttcgtggc tcacctgcag
2821 gagaccagcc cgctgaggga tgccgtcgtc atcgagcaga gctcctccct gaatgaggcc
2881 agcagtggcc tcttcgacgt cttcctacgc ttcatgtgcc accacgccgt gcgcatcagg
2941 ggcaagtcct acgtccagtg ccaggggatc ccgcagggct ccatcctctc cacgctgctc
3001 tgcagcctgt gctacggcga catggagaac aagctgtttg cggggattcg gcgggacggg
3061 ctgctcctgc gtttggtgga tgatttcttg ttggtgacac ctcacctcac ccacgcgaaa
3121 accttcctca ggaccctggt ccgaggtgtc cctgagtatg gctgcgtggt gaacttgcgg
3181 aagacagtgg tgaacttccc tgtagaagac gaggccctgg gtggcacggc ttttgttcag
3241 atgccggccc acggcctatt cccctggtgc ggcctgctgc tggatacccg gaccctggag
3301 gtgcagagcg actactccag ctatgcccgg acctccatca gagccagtct caccttcaac
3361 cgcggcttca aggctgggag gaacatgcgt cgcaaactct ttggggtctt gcggctgaag
3421 tgtcacagcc tgtttctgga tttgcaggtg aacagcctcc agacggtgtg caccaacatc
3481 tacaagatcc tcctgctgca ggcgtacagg tttcacgcat gtgtgctgca gctcccattt
3541 catcagcaag tttggaagaa ccccacattt ttcctgcgcg tcatctctga cacggcctcc
3601 ctctgctact ccatcctgaa agccaagaac gcagggatgt cgctgggggc caagggcgcc
3661 gccggccctc tgccctccga ggccgtgcag tggctgtgcc accaagcatt cctgctcaag
3721 ctgactcgac accgtgtcac ctacgtgcca ctcctggggt cactcaggac agcccagacg
3781 cagctgagtc ggaagctccc ggggacgacg ctgactgccc tggaggccgc agccaacccg
3841 gcactgccct cagacttcaa gaccatcctg gactgattaa ttaagtctag agtcgagtct
3901 agagggcccg cggttcgaac aaaaactcat ctcagaagag gatctgaata tgcataccgg
3961 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
4021 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
4081 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
4141 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
4201 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
4261 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4321 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
4381 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
4441 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
4501 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
4561 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
4621 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
4681 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
4741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4801 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
4861 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
4921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
4981 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
5041 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
5281 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
5641 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
5881 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga
5941 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
6001 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
6061 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
6121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
6181 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
6241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
6301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
6361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
6421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
6481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
6541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
6601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
7081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
7381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
7441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
7501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
7561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
7621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
7681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
7741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
7801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
7861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
7921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
7981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
8041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
8101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
8161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
8221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
8281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
8341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
8401 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
8461 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
8521 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
8581 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
8641 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
8701 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
8761 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
8821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
8881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
8941 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
9001 tacgtattag tcatcgctat taccatg
//
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