| 质粒名称: | pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002228 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting TP53 gene NickingRNA and pegRNA, harboring dual selection markers of puroR and RFP. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
hCtRNA
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TP53-nicking-sgRNA-pegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1载体质粒基本信息
pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1质粒载体简介
pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-TP53-Nicking/PegRNA-PGK-puroR-mRFP1 5676 bp DNA circular SYN 08-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5676)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5676
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 24..61
/label=OL2745
misc_feature 41..115
/label=hCtRNA
primer_bind 56..79
/label=OL464
primer_bind complement(96..135)
/label=OL3444
primer_bind complement(96..116)
/label=OL3446
primer_bind complement(98..118)
/label=OL465
primer_bind 116..159
/label=OL3445
misc_feature 116..135
/label=TP53-nickingRNA
primer_bind 133..176
/label=OL462
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..159
/label=OL3447
primer_bind complement(192..211)
/label=OL3448
primer_bind complement(194..230)
/label=OL463
misc_feature 212..286
/label=hCtRNA
primer_bind 212..232
/label=OL2745
primer_bind 227..250
/label=OL464
primer_bind complement(267..306)
/label=OL3446
primer_bind complement(267..287)
/label=OL3444
primer_bind complement(269..286)
/label=OL465
primer_bind 287..330
/label=OL3447
misc_feature 287..306
/label=TP53-Spacer
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 307..347
/label=OL462
primer_bind 307..330
/label=OL3445
primer_bind complement(363..434)
/label=OL3448
primer_bind complement(365..382)
/label=OL463
misc_feature 383..396
/label=RTT
primer_bind 394..410
/label=OL3446
misc_feature 397..409
/label=PBS
misc_feature 453..570
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 619..1129
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1048..1066
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1151..1747
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1151..1170)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1647..1667
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 1748..1804
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
RBS 1787..1794
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1805..2482
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(2460..2507)
/label=OL903
primer_bind 2481..2499
/label=OL965
polyA_signal 2629..2750
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2640..2659)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2694..2713
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2998..3015)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3169..3757)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3249..3268)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3928..4788)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4551..4570
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4789..4893)
/gene="bla"
/label=AmpR promoter
rep_origin 4920..5375
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5007..5026)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5217..5238
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5506..5554
/note="synthetic polyadenylation signal"
misc_feature 5568..5659
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5608..5627
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctatcag
121 aggcctgggg accctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctagca catgacggag
301 gttgtggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gcgggcagtg cctcacaacc tccgtcatgt ttttttctcg
421 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
481 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
541 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
601 ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
661 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggac
721 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
781 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
841 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
901 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
961 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
1021 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
1081 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc
1141 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
1201 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
1261 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
1321 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
1381 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
1441 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
1501 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
1561 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
1621 cgccttcctg gaaacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
1681 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
1741 cggtgccgct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
1801 acctatggcg agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
1861 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
1921 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
1981 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
2041 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
2101 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
2161 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
2221 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
2281 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
2341 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
2401 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
2461 tcgtcactcc accggtgctt aacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
2521 cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct
2581 tagccacttt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
2641 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
2701 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
2761 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
2821 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc
2881 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
2941 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
3001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
3061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
3121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
3181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
3241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
3421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
3481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
3541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
3601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
3661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
3721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
3781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
3841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
3901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
3961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
4021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc
4081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
4141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
4201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
4261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
4321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
4381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
4441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
4501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
4561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
4621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
4681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
4741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
4801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
4861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
4921 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
4981 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
5041 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
5101 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
5161 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
5221 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
5281 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
5341 cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa
5401 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg
5461 ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta
5521 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
5581 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
5641 ggtgccagaa catttctcta tcgataggta ccgatt
//
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