| 质粒名称: | pSL1597 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002451 |
| 产品用途: | Mammalian PhCMV-driven expression vector for PAL-specific SEAP-mRNA containing 16 tandem APT_PAL repeats in the 3’-UTR (PhCMV-SEAP-(APT_PAL)16-HHR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL1597
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1597载体质粒基本信息
pSL1597质粒图谱载体图谱和pSL1597载体序列质粒序列多克隆位点信息
pSL1597质粒载体简介
pSL1597质粒序列载体序列
LOCUS pSL1597 7841 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7841)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7841
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..900
/label=oSCL2270
misc_feature 895..900
/label=NheI
primer_bind 897..932
/label=Oscl31-3
misc_feature 911..915
/label=New Feature
misc_feature 911..915
/label=New Feature
misc_feature 915
/label=EGFP
/note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
[758 nt] : (KpnI[4] / XbaI[766])"
primer_bind 918..932
/label=oSCL775
primer_bind 920..932
/label=oSCL456
misc_feature 926..2485
/label=SEAP
primer_bind complement(1114..1150)
/label=oSCL545
primer_bind 1131..1159
/label=oSCL544
primer_bind complement(1947..1978)
/label=oSCL546
primer_bind 1959..1991
/label=oSCL547
primer_bind 2044..2074
/label=Oscl50-1
primer_bind complement(2044..2074)
/label=Oscl50-2
primer_bind complement(2433..2485)
/label=oSCL795
primer_bind complement(2467..2482)
/label=oSCL475
primer_bind complement(2469..2511)
/label=oSCL083
primer_bind complement(2469..2486)
/label=oSCL548
primer_bind complement(2469..2485)
/label=Oscl31-4
primer_bind complement(2469..2485)
/label=oSCL457
primer_bind complement(2469..2485)
/label=oSCL776
polyA_signal 2477..2485
/label=SV40 late polyA
misc_feature 2512..2515
/label=linker
primer_bind complement(2517..2531)
/label=Oscl28-2
primer_bind complement(2518..2531)
/label=8x R
misc_feature 2532..2544
/label=linker
misc_feature 2550..2566
/label=APT(PAL04.17)
misc_feature 2572..2581
/label=linker
misc_feature 2587..2603
/label=APT(PAL04.17)
misc_feature 2617..2645
/label=hairpin
misc_feature 2655..2683
/label=hairpin
misc_feature 2687..2692
/label=linker
misc_feature 2701..2716
/label=linker
misc_feature 2722..2725
/label=linker
primer_bind complement(2727..2741)
/label=Oscl28-2
primer_bind complement(2728..2741)
/label=8x R
misc_feature 2742..2754
/label=linker
misc_feature 2760..2776
/label=APT(PAL04.17)
misc_feature 2782..2791
/label=linker
misc_feature 2797..2813
/label=APT(PAL04.17)
misc_feature 2827..2855
/label=hairpin
misc_feature 2865..2893
/label=hairpin
misc_feature 2897..2902
/label=linker
misc_feature 2911..2926
/label=linker
misc_feature 2953..2965
/label=linker
misc_feature 2971..2987
/label=APT(PAL04.17)
misc_feature 2993..3002
/label=linker
misc_feature 3008..3024
/label=APT(PAL04.17)
misc_feature 3038..3066
/label=hairpin
misc_feature 3076..3104
/label=hairpin
misc_feature 3108..3113
/label=linker
misc_feature 3122..3137
/label=linker
misc_feature 3143..3155
/label=linker
misc_feature 3161..3177
/label=APT(PAL04.17)
misc_feature 3183..3192
/label=linker
misc_feature 3198..3214
/label=APT(PAL04.17)
misc_feature 3220..3229
/label=linker
misc_feature 3220..3229
/label=linker
misc_feature 3228..3256
/label=hairpin
misc_feature 3255..3267
/label=linker
misc_feature 3266..3294
/label=hairpin
misc_feature 3298..3303
/label=linker
misc_feature 3312..3327
/label=linker
misc_feature 3334..3409
/label=HHR Env140_nutR_H6
misc_feature complement(3342..3350)
/label=Stem III
misc_feature 3353..3368
/label=Stem I
terminator 3377..3391
/label=nutR-boxB
misc_feature 3395..3403
/label=Stem III
primer_bind complement(3410..3424)
/label=oSCL2271
terminator 3438..3665
/label=bGH PA
polyA_signal 3441..3665
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3711..4139
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4153..4482
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4333..4468
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 4333..4410
/label=SV40 ORI
CDS 4549..5343
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5517..5638
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5683..5703)
/label=M13-rev
primer_bind complement(5687..5703)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5709..5731)
/label=LacO
protein_bind 5711..5727
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5735..5765)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5736..5765)
/label=lac
protein_bind 5780..5801
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6089..6674)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6845..7705)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7706..7810)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttagcg ccaccatgct gctgctgctg ctgctgctgg gcctgaggct
961 acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
1021 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
1081 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccagaatcct
1141 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
1201 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
1261 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
1321 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
1381 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
1441 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
1501 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
1561 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
1621 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
1681 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
1741 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
1801 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
1861 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
1921 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacca tcatgttcga
1981 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
2041 tgccgaccac tcccacgtgt tctccttcgg aggctacccc ctgcgaggga gctccatctt
2101 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
2161 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
2221 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
2281 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
2341 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
2401 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggttacagca gagtcggggc
2461 ggccggccgc ttcgagcaga catgagcggc cgctcaatct cgaggggatc caacttgaac
2521 cggtaagaat tgcaacaaac aaatcaccag gttgaagcag acgacctggt gaaagcctta
2581 gcctagggtt gaagcagacg acccaggcac aagaaattct acggttgaag cagacgaccg
2641 tagaatcaca aagactacag ggttgaagca gacgaccctg tagactcaaa cttaaaaagg
2701 atatcttcag caaactgatc caacttgaac cggtaagaat tgcaacaaac aaatcaccag
2761 gttgaagcag acgacctggt gaaagcctta gcctagggtt gaagcagacg acccaggcac
2821 aagaaattct acggttgaag cagacgaccg tagaatcaca aagactacag ggttgaagca
2881 gacgaccctg tagactcaaa cttaaaaagg atatcttcag caaactgatc ggagacgagt
2941 ccgtctcaaa ttgcaacaaa caaatcacca ggttgaagca gacgacctgg tgaaagcctt
3001 agcctagggt tgaagcagac gacccaggca caagaaattc tacggttgaa gcagacgacc
3061 gtagaatcac aaagactaca gggttgaagc agacgaccct gtagactcaa acttaaaaag
3121 gatatcttca gcaaactgaa ttgcaacaaa caaatcacca ggttgaagca gacgacctgg
3181 tgaaagcctt agcctagggt tgaagcagac gacccaggca caagaaattc tacggttgaa
3241 gcagacgacc gtagaatcac aaagactaca gggttgaagc agacgaccct gtagactcaa
3301 acttaaaaag gatatcttca gcaaactgaa ttcgtttaaa ccctcctcgt ccggggctgt
3361 gtcgccccgc tgacgagccc tgaaaaaggg cgaaacgagg aggactagtg ggcccgttta
3421 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
3481 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
3541 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
3601 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
3661 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
3721 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3781 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3841 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3901 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
3961 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4021 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
4081 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
4141 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
4201 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
4261 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
4321 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
4381 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
4441 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
4501 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
4561 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
4621 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
4681 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
4741 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
4801 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
4861 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
4921 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
4981 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
5041 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
5101 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
5161 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
5221 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
5281 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
5341 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
5401 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
5461 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
5521 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
5581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
5641 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
5701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
5761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
5821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
5881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
5941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
6001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
6061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
6121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
6181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
6241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
6301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
6361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
6421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
6481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
6541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
6601 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
6661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
6721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
6781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
6841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
6901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
6961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
7021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
7081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
7141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
7201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
7261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
7321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
7381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
7441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
7501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
7561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
7621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
7681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
7741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
7801 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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pSL1597质粒载体应用举例
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