psiCHECK-2-h1-cluster27

LOCUS       psiCHECK-2-h1-cluster27               6814 bp DNA     circular SYN 02-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2213
                     /label=h1-cluster27
     polyA_signal    2229..2277
                     /note="synthetic polyadenylation signal"
     promoter        2286..3037
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3067..3076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3073..4725
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             4032..4040
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4769..4890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5023..5127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5128..5988
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6159..6747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 gttaggtccc cactgctttt attttctgga agacattcat aaggaaagat gtatcttcat
     1741 gcttcagcca aaacccagat gggaataaga ccttctatta tacaggattc cccagagaaa
     1801 cagaaccaat agtgaggggg tcaggggaag aggcagttgg gagagaggga gaaagagaga
     1861 gaggctttat cttaaggaac tggctcattc aattttagag tttggcaagt ccaaaatttg
     1921 cagagtagcc cagtagactg gagacccagg aaatacttga tgttcagtcc aaaggcactc
     1981 tgctggcaaa attccctctt cttcagggga ggtctttttt ctcttaaggc ttctaactga
     2041 ttggataaag cccacctagt gacctcattg taatttaatt acttctttaa agacattgtc
     2101 tccaaataca atctcattct ggggtgctaa gggttaacaa cttcaacata tgaatttaag
     2161 gggacacaat ttcacccata aaatacctca gtgggtttta ttatgatcta gatgcggccg
     2221 ctggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgagg
     2281 atctaaatga gtcttcggac ctcgcggggg ccgcttaagc ggtggttagg gtttgtctga
     2341 cgcgggggga gggggaagga acgaaacact ctcattcgga ggcggctcgg ggtttggtct
     2401 tggtggccac gggcacgcag aagagcgccg cgatcctctt aagcaccccc ccgccctccg
     2461 tggaggcggg ggtttggtcg gcgggtggta actggcgggc cgctgactcg ggcgggtcgc
     2521 gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc ccgggcggcg ccgccgcggc
     2581 ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt atacgtggac aggctctgga
     2641 gcatccgcac gactgcggtg atattaccgg agaccttctg cgggacgagc cgggtcacgc
     2701 ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac ggggcacagg tacactatct
     2761 tgtcacccgg aggcgcgagg gactgcagga gcttcaggga gtggcgcagc tgcttcatcc
     2821 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2881 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     2941 agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg
     3001 cctcgaacac cgagcgaccc tgcagcgacc cgcttaaaag cttggcattc cggtactgtt
     3061 ggtaaagcca ccatggccga tgctaagaac attaagaagg gccctgctcc cttctaccct
     3121 ctggaggatg gcaccgctgg cgagcagctg cacaaggcca tgaagaggta tgccctggtg
     3181 cctggcacca ttgccttcac cgatgcccac attgaggtgg acatcaccta tgccgagtac
     3241 ttcgagatgt ctgtgcgcct ggccgaggcc atgaagaggt acggcctgaa caccaaccac
     3301 cgcatcgtgg tgtgctctga gaactctctg cagttcttca tgccagtgct gggcgccctg
     3361 ttcatcggag tggccgtggc ccctgctaac gacatttaca acgagcgcga gctgctgaac
     3421 agcatgggca tttctcagcc taccgtggtg ttcgtgtcta agaagggcct gcagaagatc
     3481 ctgaacgtgc agaagaagct gcctatcatc cagaagatca tcatcatgga ctctaagacc
     3541 gactaccagg gcttccagag catgtacaca ttcgtgacat ctcatctgcc tcctggcttc
     3601 aacgagtacg acttcgtgcc agagtctttc gacagggaca aaaccattgc cctgatcatg
     3661 aacagctctg ggtctaccgg cctgcctaag ggcgtggccc tgcctcatcg caccgcctgt
     3721 gtgcgcttct ctcacgcccg cgaccctatt ttcggcaacc agatcatccc cgacaccgct
     3781 attctgagcg tggtgccatt ccaccacggc ttcggcatgt tcaccaccct gggctacctg
     3841 atttgcggct ttcgggtggt gctgatgtac cgcttcgagg aggagctgtt cctgcgcagc
     3901 ctgcaagact acaaaattca gtctgccctg ctggtgccaa ccctgttcag cttcttcgct
     3961 aagagcaccc tgatcgacaa gtacgacctg tctaacctgc acgagattgc ctctggcggc
     4021 gccccactgt ctaaggaggt gggcgaagcc gtggccaagc gctttcatct gccaggcatc
     4081 cgccagggct acggcctgac cgagacaacc agcgccattc tgattacccc agagggcgac
     4141 gacaagcctg gcgccgtggg caaggtggtg ccattcttcg aggccaaggt ggtggacctg
     4201 gacaccggca agaccctggg agtgaaccag cgcggcgagc tgtgtgtgcg cggccctatg
     4261 attatgtccg gctacgtgaa taaccctgag gccacaaacg ccctgatcga caaggacggc
     4321 tggctgcact ctggcgacat tgcctactgg gacgaggacg agcacttctt catcgtggac
     4381 cgcctgaagt ctctgatcaa gtacaagggc taccaggtgg ccccagccga gctggagtct
     4441 atcctgctgc agcaccctaa cattttcgac gccggagtgg ccggcctgcc cgacgacgat
     4501 gccggcgagc tgcctgccgc cgtcgtcgtg ctggaacacg gcaagaccat gaccgagaag
     4561 gagatcgtgg actatgtggc cagccaggtg acaaccgcca agaagctgcg cggcggagtg
     4621 gtgttcgtgg acgaggtgcc caagggcctg accggcaagc tggacgcccg caagatccgc
     4681 gagatcctga tcaaggctaa gaaaggcggc aagatcgccg tgtaataatt ctagagtcgg
     4741 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     4801 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4861 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4921 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4981 aaaatcgata aggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5041 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5101 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5161 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5221 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5281 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5341 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5401 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5461 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5521 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5581 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5641 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5701 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5761 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5821 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5881 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5941 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6781 gccttttgct ggccttttgc tcacatggct cgac
//