pM-Cas12a

LOCUS       pM-Cas12a       12991 bp DNA     circular SYN 25-AUG-2024
DEFINITION  35S promoter-driven expression of the positive-sense TSWV M RNA 
            segment encoding LbCas12a in place of viral GP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   Liu Q, Zhao C, Sun K, Deng Y, Li Z
  TITLE     Engineered biocontainable RNA virus vectors for non-transgenic 
            genome editing across crop species and genotypes.
  JOURNAL   Mol Plant. 2023 Mar 6;16(3):616-631. doi: 
            10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
  PUBMED    36751129
REFERENCE   2  (bases 1 to 12991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date: 
            "2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
FEATURES             Location/Qualifiers
     source          1..12991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        423..768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     711..731
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             853..876
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             907..927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4633..4680
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6060..6312
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6360..6384
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6512..7854
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7475..7522)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7895..7913
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7951..7973)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8073..8092
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8113..8394
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8395..9069
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9172..9627)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9309..9330)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9521..9540
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(9743..10891)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11190..11984)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12193..12824
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    12904..12928
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aatcagtgca
      781 aacaaaaacc ttaatccaga catcttgaaa ttaatcatat aaccattata atctgagcag
      841 acgtataaga tggattacaa ggatgacgac gataagcccg ggggatcctc tagagtcgac
      901 atggctccta agaagaagcg gaaggttggt attcacgggg tgcctgcggc ttcaaagctc
      961 gagaaattca ccaactgtta ttcgttgagc aaaacactgc ggtttaaagc gattccagtc
     1021 ggcaagactc aagagaatat agacaataag cggctgttgg tggaagatga aaagcgcgcg
     1081 gaagactaca aaggggtgaa gaagttgttg gacagatact acctctcttt tatcaatgat
     1141 gtcttgcact caatcaaatt gaagaatctg aacaactaca tctccctctt cagaaagaaa
     1201 acaaggacag aaaaggagaa taaggaactt gaaaatttgg agatcaatct gaggaaagag
     1261 atcgcgaaag cctttaaagg caacgaagga tacaaaagtc tgttcaagaa ggatataatt
     1321 gagacaattt tgccagagtt cctcgatgac aaggacgaga ttgcgctggt caattcgttc
     1381 aacggattca caacagcatt cacaggcttc tttgataatc gggaaaatat gttctctgag
     1441 gaggcaaagt ccacttctat tgcgttcagg tgtatcaatg agaatctcac taggtacatt
     1501 tccaacatgg atatctttga gaatgttgac gcaatttttg acaagcacga agttcaggag
     1561 attaaggaga agatcctcaa ttccgattat gacgttgagg acttcttcga aggtgagttt
     1621 tttaatttcg tgctcactca agagggtatc gacgtgtata atgcgatcat cggtgggttc
     1681 gtgactgagt ccggtgaaaa gattaaggga ttgaacgagt atatcaacct ttacaaccaa
     1741 aagacgaaac agaagctgcc aaagttcaag cctctttaca aacaggttct ttcagaccgc
     1801 gagtcactct cgttctatgg ggagggctac acttcggatg aggaagtcct ggaggtgttc
     1861 aggaatactc tcaataagaa ttcggagatt ttctcttcta taaaaaaact ggaaaagttg
     1921 tttaagaatt ttgacgaata ctctagcgcc ggcatatttg tgaaaaacgg cccggccata
     1981 tcaacgataa gtaaagatat cttcggcgaa tggaacgtga tcagagacaa atggaacgcg
     2041 gagtatgacg atattcacct gaagaagaag gctgtcgtaa cggagaagta cgaggatgat
     2101 cgcaggaaaa gcttcaaaaa gatcggaagt ttcagcctgg aacagttgca ggagtatgct
     2161 gacgccgatc ttagcgtcgt cgagaagttg aaggagataa tcatccaaaa ggtcgacgag
     2221 atatataaag tctatggatc aagtgaaaaa ctgttcgacg ccgacttcgt tttggagaag
     2281 tccctgaaga agaacgacgc tgttgttgcc attatgaagg atctgctcga cagcgtgaag
     2341 agtttcgaga actatattaa ggcttttttc ggggagggga aggagactaa cagagatgag
     2401 tccttctacg gagacttcgt cctcgcgtac gatatactcc ttaaggtaga ccacatctac
     2461 gacgcaatca gaaattacgt gacacaaaag ccgtacagca aggacaagtt caaactctac
     2521 ttccagaacc cccagttcat gggcggctgg gacaaggaca aggaaacgga ttacagggct
     2581 acgatcctga ggtatggttc aaaatactac ttggcgatta tggacaagaa gtacgccaag
     2641 tgtctccaga agattgacaa agacgatgtc aatggcaatt atgagaagat caactacaag
     2701 ctgcttccgg gtccgaacaa gatgctccca aaggttttct tcagcaagaa atggatggcc
     2761 tactataacc caagcgagga catccagaag atttataaga acggtacgtt caagaagggc
     2821 gacatgttca atcttaacga ctgtcacaag ctgatcgact tcttcaaaga ctcaattagc
     2881 cggtacccaa agtggtctaa cgcctatgac ttcaactttt cggaaaccga gaagtacaag
     2941 gatatagccg gattttatag agaggtggaa gagcagggct acaaggtgtc attcgagtcc
     3001 gccagcaaga aggaagtgga caagctcgtg gaagatggta agctctacat gttccagatt
     3061 tataataaag actttagcga taagagccac gggacaccta atctccacac aatgtatttc
     3121 aagctgctct tcgacgagaa taaccacggc caaatcaggt tgtcaggagg ggctgaactc
     3181 ttcatgcggc gcgctagcct taagaaggag gagcttgtag tccaccctgc gaatagtcca
     3241 attgcgaata agaacccgga caatcctaaa aagactacaa cattgagcta cgacgtgtac
     3301 aaggataaga ggttttccga ggatcagtac gagctccaca tcccgattgc gatcaacaag
     3361 tgcccaaaga atattttcaa gataaacaca gaggtgcgtg tactcctgaa gcatgacgac
     3421 aatccttacg tcattgggat tgatcggggc gagaggaacc tcctctatat tgtggtggtg
     3481 gacgggaagg ggaacatagt cgaacagtac tcccttaacg aaataattaa caatttcaac
     3541 ggcatccgta tcaagaccga ctaccattcg ttgctggaca agaaggagaa ggagagattt
     3601 gaggcgcggc aaaattggac aagtatcgag aacatcaagg aactcaaagc aggttatatc
     3661 tctcaagttg tgcataagat atgcgagctg gttgagaagt atgacgcagt gatcgctctt
     3721 gaggacctca actcgggctt taagaattct agagttaaag tggagaagca ggtctatcaa
     3781 aagttcgaga agatgcttat agataagctc aactacatgg tcgataagaa atcgaaccca
     3841 tgtgccaccg gcggcgcact caaaggttac caaataacaa acaaattcga gtccttcaaa
     3901 tcgattagta ctcagaatgg gttcatattt tatataccgg cgtggcttac gtctaagatc
     3961 gacccgtcaa ctggttttgt caacctgttg aagacgaaat acacgtccat tgccgattcg
     4021 aaaaagttca tatctagttt tgatcgtatt atgtacgtcc cagaggaaga tcttttcgag
     4081 tttgctctcg actacaaaaa cttttcgcgg accgatgcgg attacattaa aaaatggaaa
     4141 ctctattcgt acggcaacag aatcaggatt tttcgcaacc ctaagaagaa taacgtcttt
     4201 gattgggagg aagtttgctt gactagcgcg tacaaggagc tctttaataa gtatggcatt
     4261 aactaccaac agggtgatat cagagcactg ctttgcgaac aatctgacaa ggctttctac
     4321 tcatccttca tggctttgat gagcctgatg ctccagatga gaaattcaat tacaggcaga
     4381 accgacgtgg atttcttgat ctccccggtt aaaaattctg atggcatctt ttacgatagc
     4441 aggaactatg aagcgcaaga gaatgcgatt ctgccaaaaa atgcagacgc caacggtgcc
     4501 tataacatcg ccaggaaagt cctgtgggcg atcggccagt tcaaaaaggc cgaagacgaa
     4561 aaattggaca aggtcaaaat cgctatcagc aacaaagagt ggctggagta tgctcagaca
     4621 tccgtaaagc ataagcgtcc tgctgccacc aaaaaggccg gacaggctaa gaaaaagaag
     4681 tgaggctgct tgtcacaagc taaatttgat cctatttcct acattaatcg aattatatat
     4741 atataaaata aacaaaaaac aaacaaaaat acaaataaac aaaaaacaac aaaaagagtc
     4801 ttcggaccaa agtttggctt ttcagcctta ttttgttttt gttttttgtt tttgattttt
     4861 tgtttgtttt ttatttttgt ttttgtttgg tttttgattt aaaaatgcaa aataaacaga
     4921 gatttaagct taaataagtg gtattaaaag tactatattt catcaaaaga taactgagca
     4981 acattatcag aaaccccttc ctcctcttct tcaactgatc tctcaagatt tgagctcagt
     5041 tctttaagct gttttttaat ctgcttctca ctgtttcctt taggaattat cagcttgcaa
     5101 gcctcaatga atgcttgaga tctagctcta atagccctgt ttagaggtat gaccatgcaa
     5161 cttttgtctt tatcagctct gggtgaatca caaaactcct ttgtccaaga atacatgaca
     5221 ctaccaaaag aaaccccctt cttgtattct tgactgcaca tcaagtgcag ctgacagcag
     5281 ttttctggag tgttattcat tttcggaata gaccagttca gataaaaaac aaaacagata
     5341 ggatcagtta ttgtcccctg acccttcaga atgatttgct tttcagatgg catgttggga
     5401 tcgaccagag caaccacaag tttccctgta gggtttggta tagtggggca gacccatatc
     5461 acaatcctgg aaatcatcat gtattgtttt ctgctgtccc aagttggaca gatcttgatg
     5521 accttattag cgttttgctt cccgttgccg acaaaaagat catttttcca gtttgagata
     5581 tggtggtttg tatctactat cattcttgca gaaagatcat aaccctctga ttctgtgata
     5641 gaatcagatt catatgttcc aaaggaagat gttccctctg tgttcaacag tatctttcct
     5701 ttggatgtat ccatagcttt ggttaaagca agcttttcat cagaagaaga ccatggtttt
     5761 gagacttcaa cattaccgtt atgtttagca agtgaaacta aaggcccttc atcctttctg
     5821 gcagacttag aagacccctt attaccaaaa aaagtcaaca ttttgagttc aacagcctga
     5881 ggtagaggag cttgtgtaat gtaattgatt gattcttagc aaagtgtata ataggtatat
     5941 ttctgatgca ctgattgctc tgggtcggca tggcatctcc acctcctcgc ggtccgacct
     6001 gggcatccga aggaggacgt cgtccactcg gatggctaag ggagagctcg aatttccccg
     6061 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     6121 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     6181 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     6241 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     6301 tgttactaga tcggaattca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
     6361 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     6421 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     6481 gagatctcag taaagcgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga
     6541 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     6601 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     6661 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     6721 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     6781 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     6841 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     6901 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     6961 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     7021 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     7081 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     7141 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     7201 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     7261 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     7321 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     7381 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     7441 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
     7501 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     7561 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     7621 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     7681 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     7741 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     7801 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta
     7861 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7921 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     7981 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8041 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     8101 gcaccataga tcaacgacat tactatatat ataatatagg aagcatttaa tagaacagca
     8161 tcgtaatata tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt
     8221 tattgaaaaa tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc
     8281 cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg
     8341 tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct
     8401 gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca
     8461 gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc
     8521 aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca
     8581 tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag
     8641 gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat
     8701 gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt
     8761 ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc
     8821 ttgtttgatt cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac
     8881 tgggttggaa ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg
     8941 ccagaaaatg ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga
     9001 ggtgtggaga caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct
     9061 aagaaatagg ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct
     9121 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     9181 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     9241 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     9301 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     9361 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     9421 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     9481 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     9541 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     9601 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     9661 agggcgatcg gtgcgggcct cttcgctatt acgccagatc cttgctggct gaacccccag
     9721 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
     9781 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
     9841 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
     9901 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
     9961 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
    10021 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
    10081 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
    10141 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
    10201 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
    10261 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
    10321 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
    10381 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
    10441 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
    10501 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
    10561 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
    10621 tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg
    10681 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
    10741 tagcttgctg ggccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
    10801 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
    10861 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
    10921 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
    10981 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
    11041 tggtattccg aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt
    11101 gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt
    11161 cgccggcatc gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat
    11221 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    11281 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    11341 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    11401 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    11461 aaaaaatcat acagctcgcg cggatcttta aatggggtgt cttcttccca gttttcgcaa
    11521 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    11581 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    11641 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    11701 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaag gtgcaggacc
    11761 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    11821 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    11881 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    11941 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    12001 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    12061 cactgttcct tgcattctaa aactttaaat accagaaaac agctttttca aagttgtttt
    12121 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga
    12181 tgctgcaact cgagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc
    12241 gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa
    12301 aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc
    12361 tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag
    12421 cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca
    12481 agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg
    12541 cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa
    12601 atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa
    12661 cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc
    12721 ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga
    12781 cggccgccag cccagcggcg agggcaacca gcccggtgag cgtctctagt ggactgatgg
    12841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    12901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
    12961 gcggacgttt ttaatgtact ggggtggttt t
//