| 质粒名称: | pCE-hSK |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41814 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCE |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9364 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SOX2, KLF4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2513 bp |
| 基因突变: | -- |
| GenBank编号 | NM_003106 NM_004235 |
| 启动子 | CAG |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | pCAG-F |
| 3' 测序引物 | TTAGCCAGAAGTCAGATGCTC |
|
文献引用方法 |
|
| 材料和方法部分: | pCE-hSK was a gift from Shinya Yamanaka (Addgene plasmid # 41814 ; http://n2t.net/addgene:41814 ; RRID:Addgene_41814) |
| 参考文献部分: | An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. 10.1002/stem.1293 PubMed 23193063 |
买家导航
pCE-hSK载体 pCEhSK质粒基本信息
pCE-hSK质粒图谱 pCEhSK载体图谱和pCE-hSK载体序列 pCEhSK质粒序列多克隆位点信息
pCE-hSK质粒 pCEhSK载体简介
pCE-hSK质粒序列 pCEhSK载体序列
LOCUS pCE-hSK 11877 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Non-integrating (episomal) expression of human SOX2 and KLF4.
ACCESSION .
VERSION .
KEYWORDS pCE-hSK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11877)
AUTHORS Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A,
Goshima N, Yamanaka S
TITLE An Efficient Non-viral Method to Generate Integration-Free Human iPS
Cells from Cord Blood and Peripheral Blood Cells.
JOURNAL Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
PUBMED 23193063
REFERENCE 2 (bases 1 to 11877)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11877
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(83..102)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 148..203
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(202..221)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(564..580)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(564..580)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(577..599)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 588..604
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(612..642)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 657..678
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 1157..3082
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
rep_origin 3384..5173
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(5715..5732)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5886..6474)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5966..5985)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6645..7505)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7268..7287
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7506..7610)
/gene="bla"
/label=AmpR promoter
enhancer 7641..8020
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8022..8298
/label=chicken beta-actin promoter
intron 8299..9315
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 9239..9261
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 9323..9342
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 10355..10420
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
1 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
61 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
121 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
181 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
241 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
301 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
361 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
421 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
481 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
541 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
721 tcgtgccagc ggatctcaat tctcatgttt gacagcttat catcgataag ctgatcctca
781 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa
841 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag
901 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt
961 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca
1021 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg
1081 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc
1141 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg
1201 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg
1261 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg
1321 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa
1381 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc
1441 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
1501 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg
1561 ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg
1621 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc
1681 aggagcagga ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagg
1741 ggcaggagca ggaggagggg caggagcagg aggggcagga ggggcaggag gggcaggagc
1801 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggggc
1861 aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag gggcaggagc
1921 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggaggggc
1981 aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggagggg caggaggggc
2041 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
2101 aggggcagga gcaggagggg caggagcagg aggtggaggc cggggtcgag gaggcagtgg
2161 aggccggggt cgaggaggta gtggaggccg gggtcgagga ggtagtggag gccgccgggg
2221 tagaggacgt gaaagagcca gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg
2281 acgtggagaa aagaggccca ggagtcccag tagtcagtca tcatcatccg ggtctccacc
2341 gcgcaggccc cctccaggta gaaggccatt tttccaccct gtaggggaag ccgattattt
2401 tgaataccac caagaaggtg gcccagatgg tgagcctgac gtgcccccgg gagcgataga
2461 gcagggcccc gcagatgacc caggagaagg cccaagcact ggaccccggg gtcagggtga
2521 tggaggcagg cgcaaaaaag gagggtggtt tggaaagcat cgtggtcaag gaggttccaa
2581 cccgaaattt gagaacattg cagaaggttt aagagctctc ctggctagga gtcacgtaga
2641 aaggactacc gacgaaggaa cttgggtcgc cggtgtgttc gtatatggag gtagtaagac
2701 ctccctttac aacctaaggc gaggaactgc ccttgctatt ccacaatgtc gtcttacacc
2761 attgagtcgt ctcccctttg gaatggcccc tggacccggc ccacaacctg gcccgctaag
2821 ggagtccatt gtctgttatt tcatggtctt tttacaaact catatatttg ctgaggtttt
2881 gaaggatgcg attaaggacc ttgttatgac aaagcccgct cctacctgca atatcagggt
2941 gactgtgtgc agctttgacg atggagtaga tttgcctccc tggtttccac ctatggtgga
3001 aggggctgcc gcggagggtg atgacggaga tgacggagat gaaggaggtg atggagatga
3061 gggtgaggaa gggcaggagt gatgtaactt gttaggagac gccctcaatc gtattaaaag
3121 ccgtgtattc ccccgcacta aagaataaat ccccagtaga catcatgcgt gctgttggtg
3181 tatttctggc catctgtctt gtcaccattt tcgtcctccc aacatggggc aattgggcat
3241 acccatgttg tcacgtcact cagctccgcg ctcaacacct tctcgcgttg gaaaacatta
3301 gcgacattta cctggtgagc aatcagacat gcgacggctt tagcctggcc tccttaaatt
3361 cacctaagaa tgggagcaac cagcaggaaa aggacaagca gcgaaaattc acgccccctt
3421 gggaggtggc ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct
3481 gactgtatat gcatgaggat agcatatgct acccggatac agattaggat agcatatact
3541 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
3601 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
3661 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
3721 acccagatat agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc
3781 agatataaat taggatagca tatactaccc taatctctat taggatagca tatgctaccc
3841 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
3901 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
3961 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
4021 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg
4081 caacattagc ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc
4141 ttggcgctca ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta
4201 tcttggcccg cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg
4261 gcaagtggtt tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta
4321 ttttacagtc caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa
4381 tttcaaaaaa aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg
4441 tttaattttc gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct
4501 ctttcccctt gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg
4561 ggacacatct taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca
4621 taaattcgtg tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta
4681 ttgttaaaga tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg
4741 tgagggttat attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat
4801 tctgggggcg tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca
4861 actcagcagt tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg
4921 agagttcact gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact
4981 accctcgtgg aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat
5041 atcgctgttc cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg
5101 gtaacatatg ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca
5161 tatgctaccc gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt
5221 gagggtccgc ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg
5281 tagtcttcct gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc
5341 aagagttaca cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag
5401 gatgaaagcc actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag
5461 tcagagaacc cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg
5521 gcaagttgta ccaaccaact gaagggatta catgcactgc cccgcgaaga aggggcagag
5581 atgtcgtagt caggtttagt tcgtccgggg cggggcatcg atcctctaga gtcgacgcta
5641 gcggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
5701 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
5761 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
5821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
5881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
5941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
6001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
6061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
6121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
6181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
6241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
6301 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
6361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
6421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
6481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
6541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
6601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
6661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
6721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
6781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
6841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
6901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
6961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
7021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
7081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
7141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
7201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
7261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
7321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
7381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
7441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
7501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
7561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
7621 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
7681 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
7741 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
7801 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
7861 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
7921 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
7981 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
8041 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
8101 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
8161 ggggcggggc gaggggcggg gcgggcgagg cggagaggtg cggcggcagc caatcagagc
8221 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
8281 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
8341 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
8401 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
8461 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
8521 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
8581 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
8641 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
8701 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
8761 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
8821 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
8881 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
8941 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
9001 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
9061 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
9121 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
9181 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
9241 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
9301 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
9361 aaagaattcg cccttcacca tgtacaacat gatggagacg gagctgaagc cgccgggccc
9421 gcagcaaact tcggggggcg gcggcggcaa ctccaccgcg gcggcggccg gcggcaacca
9481 gaaaaacagc ccggaccgcg tcaagcggcc catgaatgcc ttcatggtgt ggtcccgcgg
9541 gcagcggcgc aagatggccc aggagaaccc caagatgcac aactcggaga tcagcaagcg
9601 cctgggcgcc gagtggaaac ttttgtcgga gacggagaag cggccgttca tcgacgaggc
9661 taagcggctg cgagcgctgc acatgaagga gcacccggat tataaatacc ggccccggcg
9721 gaaaaccaag acgctcatga agaaggataa gtacacgctg cccggcgggc tgctggcccc
9781 cggcggcaat agcatggcga gcggggtcgg ggtgggcgcc ggcctgggcg cgggcgtgaa
9841 ccagcgcatg gacagttacg cgcacatgaa cggctggagc aacggcagct acagcatgat
9901 gcaggaccag ctgggctacc cgcagcaccc gggcctcaat gcgcacggcg cagcgcagat
9961 gcagcccatg caccgctacg acgtgagcgc cctgcagtac aactccatga ccagctcgca
10021 gacctacatg aacggctcgc ccacctacag catgtcctac tcgcagcagg gcacccctgg
10081 catggctctt ggctccatgg gttcggtggt caagtccgag gccagctcca gcccccctgt
10141 ggttacctct tcctcccact ccagggcgcc ctgccaggcc ggggacctcc gggacatgat
10201 cagcatgtat ctccccggcg ccgaggtgcc ggaacccgcc gcccccagca gacttcacat
10261 gtcccagcac taccagagcg gcccggtgcc cggcacggcc attaacggca cactgcccct
10321 ctcacacatg ggggatctca aaattgtcgc tcctgtcaaa caaactctta actttgattt
10381 actcaaactg gctggggatg tagaaagcaa tccaggtcca ctcatgaaca attcgccctt
10441 caccatggct gtcagtgacg cgctgctccc atctttctcc acgttcgcgt ctggcccggc
10501 gggaagggag aagacactgc gtcaagcagg tgccccgaat aaccgctggc gggaggagct
10561 ctcccacatg aagcgacttc ccccagtgct tcccggccgc ccctatgacc tggcggcggc
10621 gaccgtggcc acagacctgg agagcggcgg agccggtgcg gcttgcggcg gtagcaacct
10681 ggcgccccta cctcggagag agaccgagga gttcaacgat ctcctggacc tggactttat
10741 tctctccaat tcgctgaccc atcctccgga gtcagtggcc gccaccgtgt cctcgtcagc
10801 gtcagcctcc tcttcgtcgt cgccgtcgag cagcggccct gccagcgcgc cctccacctg
10861 cagcttcacc tatccgatcc gggccgggaa cgacccgggc gtggcgccgg gcggcacggg
10921 cggaggcctc ctctatggca gggagtccgc tccccctccg acggctccct tcaacctggc
10981 ggacatcaac gacgtgagcc cctcgggcgg cttcgtggcc gagctcctgc ggccagaatt
11041 ggacccggtg tacattccgc cgcagcagcc gcagccgcca ggtggcgggc tgatgggcaa
11101 gttcgtgctg aaggcgtcgc tgagcgcccc tggcagcgag tacggcagcc cgtcggtcat
11161 cagcgtcagc aaaggcagcc ctgacggcag ccacccggtg gtggtggcgc cctacaacgg
11221 cgggccgccg cgcacgtgcc ccaagatcaa gcaggaggcg gtctcttcgt gcacccactt
11281 gggcgctgga ccccctctca gcaatggcca ccggccggct gcacacgact tccccctggg
11341 gcggcagctc cccagcagga ctaccccgac cctgggtctt gaggaagtgc tgagcagcag
11401 ggactgtcac cctgccctgc cgcttcctcc cggcttccat ccccacccgg ggcccaatta
11461 cccatccttc ctgcccgatc agatgcagcc gcaagtcccg ccgctccatt accaagagct
11521 catgccaccc ggttcctgca tgccagagga gcccaagcca aagaggggaa gacgatcgtg
11581 gccccggaaa aggaccgcca cccacacttg tgattacgcg ggctgcggca aaacctacac
11641 aaagagttcc catctcaagg cacacctgcg aacccacaca ggtgagaaac cttaccactg
11701 tgactgggac ggctgtggat ggaaattcgc ccgctcagat gaactgacca ggcactaccg
11761 taaacacacg gggcaccgcc cgttccagtg ccaaaaatgc gaccgagcat tttccaggtc
11821 ggaccacctc gccttacaca tgaagagaca tttttaaaag ggcgaattgc ctgcagg
//
