| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-MINK1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001886 |
| 产品用途: | 5' AAVLINK plasmid for MINK1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MINK1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-MINK1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-MINK1载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-MINK1质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-MINK1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-MINK1质粒载体简介
pAAVLINK-EF1alpha-HA-5'-MINK1质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-MINK1 5766 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5766)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5766
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2906
/codon_start=1
/gene="MINK1"
/gene_synonym="B55; MAP4K6; MEKKK 6; MINK; YSK2; ZC3"
/product="misshapen-like kinase 1 isoform 3"
/EC_number="2.7.11.1"
/label=MINK1
/note="isoform 3 is encoded by transcript variant 3;
misshapen/NIK-related kinase; GCK family kinase MINK; MEK
kinase kinase 6; MAPK/ERK kinase kinase kinase 6;
mitogen-activated protein kinase kinase kinase kinase 6"
/db_xref="CCDS:CCDS45588.1"
/db_xref="GeneID:50488"
/db_xref="HGNC:HGNC:17565"
/db_xref="MIM:609426"
/protein_id="NP_722549.2"
/translation="MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHV
KTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLV
MEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTE
NAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGIT
AIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTE
QLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPS
SIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIE
EQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEE
QRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKP
AWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQE
GPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGA
VIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVR
ARPRSNSAWQIYLQRRAERGTPKPP"
misc_feature 933..938
/label=SDS
misc_feature 1125..1130
/label=SDS
misc_feature 1728..1733
/label=SDS
misc_feature 1953..1958
/label=SDS
misc_feature 2064..2069
/label=SDS
misc_feature 2444..2449
/label=SDS
misc_feature 2472..2477
/label=SDS
intron 2907..2947
/label=Splicing Donor
misc_feature 2951..2984
/label=lox2272-JT1571
repeat_region 3029..3169
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3029..3158
/label=AAV2 ITR
repeat_region 3030..3170
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3244..3699
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3981..4085
/gene="bla"
/label=AmpR promoter
CDS 4086..4946
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5117..5705
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gggcgaccca gcccccgccc gcagcctgga
781 cgacatcgac ctgtccgccc tgcgggaccc tgctgggatc tttgagcttg tggaggtggt
841 cggcaatgga acctacggac aggtgtacaa gggtcggcat gtcaagacgg ggcagctggc
901 tgccatcaag gtcatggatg tcacggagga cgaggaggaa gagatcaaac aggagatcaa
961 catgctgaaa aagtactctc accaccgcaa catcgccacc tactacggag ccttcatcaa
1021 gaagagcccc ccgggaaacg atgaccagct ctggctggtg atggagttct gtggtgctgg
1081 ttcagtgact gacctggtaa agaacacaaa aggcaacgcc ctgaaggagg actgtatcgc
1141 ctatatctgc agggagatcc tcaggggtct ggcccatctc catgcccaca aggtgatcca
1201 tcgagacatc aaggggcaga atgtgctgct gacagagaat gctgaggtca agctagtgga
1261 ttttggggtg agtgctcagc tggaccgcac cgtgggcaga cggaacactt tcattgggac
1321 tccctactgg atggctccag aggtcatcgc ctgtgatgag aaccctgatg ccacctatga
1381 ttacaggagt gatatttggt ctctaggaat cacagccatc gagatggcag agggagcccc
1441 ccctctgtgt gacatgcacc ccatgcgagc cctcttcctc attcctcgga accctccgcc
1501 caggctcaag tccaagaagt ggtctaagaa gttcattgac ttcattgaca catgtctcat
1561 caagacttac ctgagccgcc cacccacgga gcagctactg aagtttccct tcatccggga
1621 ccagcccacg gagcggcagg tccgcatcca gcttaaggac cacattgacc gatcccggaa
1681 gaagcggggt gagaaagagg agacagaata tgagtacagc ggcagcgagg aggaagatga
1741 cagccatgga gaggaaggag agccaagctc catcatgaac gtgcctggag agtcgactct
1801 acgccgggag tttctccggc tccagcagga aaataagagc aactcagagg ctttaaaaca
1861 gcagcagcag ctgcagcagc agcagcagcg agaccccgag gcacacatca aacacctgct
1921 gcaccagcgg cagcggcgca tagaggagca gaaggaggag cggcgccgcg tggaggagca
1981 acagcggcgg gagcgggagc agcggaagct gcaggagaag gagcagcagc ggcggctgga
2041 ggacatgcag gctctgcggc gggaggagga gcggcggcag gcggagcgtg agcaggaata
2101 caagcggaag cagctggagg agcagcggca gtcagaacgt ctccagaggc agctgcagca
2161 ggagcatgcc tacctcaagt ccctgcagca gcagcaacag cagcagcagc ttcagaaaca
2221 gcagcagcag cagctcctgc ctggggacag gaagcccctg taccattatg gtcggggcat
2281 gaatcccgct gacaaaccag cctgggcccg agaggtagaa gagagaacaa ggatgaacaa
2341 gcagcagaac tctcccttgg ccaagagcaa gccaggcagc acggggcctg agccccccat
2401 cccccaggcc tccccagggc ccccaggacc cctttcccag actcctccta tgcagaggcc
2461 ggtggagccc caggagggac cgcacaagag cctggtggca caccgggtcc cactgaagcc
2521 atatgcagca cctgtacccc gatcccagtc cctgcaggac cagcccaccc gaaacctggc
2581 tgccttccca gcctcccatg accccgaccc tgccatcccc gcacccactg ccacgcccag
2641 tgcccgagga gctgtcatcc gccagaattc agaccccacc tctgaaggac ctggccccag
2701 cccgaatccc ccagcctggg tccgcccaga taacgaggcc ccacccaagg tgcctcagag
2761 gacctcatct atcgccactg cccttaacac cagtggggcc ggagggtccc ggccagccca
2821 ggcagtccgt gccagacctc gcagcaactc cgcctggcaa atctatctgc aaaggcgggc
2881 agagcggggc accccaaagc ctccaggtaa gtatcaaggt tacaagacag gtttaaggag
2941 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
3001 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
3061 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
3121 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
3181 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
3241 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3301 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
3361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3421 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
3481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3541 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
3601 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
3661 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
3721 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
3781 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
3841 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
3901 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
3961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
4141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
4201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
4261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
4381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
4441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
4501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
4561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
4981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
5041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
5101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
5161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
5221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
5281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
5581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5761 acatgt
//
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