| 出品公司: | Takara |
|---|---|
| 载体名称: | pPTRI |
| 质粒类型: | 曲霉表达质粒，大肠杆菌-曲霉穿梭质粒，曲霉表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Lac |
| 载体大小: | 4782 bp |
| 5’测序引物: | M13-R: CAGGAAACAGCTATGAC |
| 3’测序引物: | M13-F: TGTAAAACGACGGCCAGT |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha |
| 宿主细胞（系）: | 曲霉菌 |
| 产品目录号: | 3621 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关载体: | pPTRII |
| 载体说明书: | pPTRI载体说明书 |
| 备注: |
本载体是曲霉表达载体，可以用于多种曲霉菌（Aspergillus species），例如A. oryzae, A . niger, A. terreus and A . fumigatus。pPTRI载体在曲霉菌中的筛选抗性是Pyrithiamine，中文名是抗硫胺素, 也叫吡啶硫铵。 本载体在构建质粒时，可以利用蓝白斑筛选进行阳性插入克隆验证。 |
买家导航
pPTRI载体 pPTR I质粒基本信息
pPTRI质粒图谱 pPTR I载体图谱和pPTRI载体序列 pPTR I质粒序列多克隆位点信息
pPTRI质粒 pPTR I载体简介
pPTR I是一种染色体整合的大肠杆菌曲霉菌穿梭载体。在曲霉菌细胞中，pPTR I不能自主复制，只有当整合到染色体上才能维持质粒稳定性。该载体具有氨苄西林抗性基因 Ampr作为大肠杆菌筛选抗性。
在曲霉中，筛选基因是抗硫胺素（Pyrithiamine） ，抗性基因为ptr A基因。本质粒具有多克隆位点，包含 Hind III，Pst I，Sma I和Kpn I。
在载体lac Z区域，任何DNA插入到这个区域 使用含有IPTG和X-Gal的平板可以很容易地进行蓝白斑筛选阳性克隆载体。
pPTRI质粒序列 pPTR I载体序列
LOCUS pPTR I Vector 4782 bp DNA circular SYN 23-JUL-2021
COMMENT Created by Takara Bio Inc. http://www.biofeng.com
COMMENT Cat. No. 3621
FEATURES Location/Qualifiers
gene complement(310..2317)
/label="ptrA"
/note="Pyrithiamine resistance gene useful as a dominant selectable marker in
Aspergillus"
CDS 2335..3195
/label="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 3366..3954
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4278..4308
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4316..4332
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4340..4356
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 4352..4678
/label="lacZ-alpha"
misc_feature 4369..4425
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(4426..4442)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
61 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
121 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
181 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
241 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
301 tttaacaaaa tggggtgacg atgagccgct cttgcatctt tgtttgtatt atactgtctt
361 tcttgttaca cataattatt ctagaatgcc ccaccgttac atacgggaca cagccattta
421 catatgcatg tggattacga gctaacgagt tcattcaaat ctcagaacta tcacataatc
481 atcattcccc tatcgtcaaa gaccgtaaga caaatccggt tcatgcactg aacccattcg
541 ggtagtgagt catttactca gcacactcgc gctgacgctc gtcgaacacc ttcaatgcct
601 cctcggcagc cttgacacca ctgagaacca tggcaccgaa ggtagggccc atgcggttaa
661 agccatcaat ttcagacagc tccataccgc cgattatcaa gcccttagta acctcgcggg
721 tgttcttgac gatggcatcc tcggccgagc tcatgtcgag accacgcatg ccacctagct
781 tgtcgacgct gcccatggac accaagcgct tcgcacagaa ggcgccgaat ggcccatcgt
841 gaccagtggt actgatgatg acaggagcgt tgatagtgtt ggggtccatg caggagtgat
901 catcgtggtg aagggtgacc agcgtccagt tgacgacaac accagcaatc tgggggttgc
961 cgttctcggt cggacgggtg atcaagtcct caacagcggt agcattgaag agcttgacat
1021 tggggaagga gagaaccttc gacatgagtg tcgaggtaaa cagggaggcg tgcttgacga
1081 caacgtagtt ggggtttgcg tcctcttcgt aaggaacacc cagctcgttc aggaagactt
1141 ccgcgggacg gcgcatgacc atagcagaaa agagttggcc acccaaccag gcaccgccac
1201 ctattgcacg ttagttccgg aaagctgagt gcaaggcaat ccatcatgga ctactgacca
1261 ggagagacgc tggcctcgac gatagcaatc ttcaggtccg gacgagcctt ggccaagacg
1321 tacgcagtgc tcagaccgca ggaaccagca ccaacaatga caacgtcact ttcagcgtac
1381 ttgtccaggt cctcaaagta acgtctggtc atggcacgag agacctggct ttcgcggata
1441 ggggcgaact tgaactcgtc ccacttgcca ccgaaatggt ccaacagctt ggtctgagaa
1501 gctccctcaa cggggacggt ctcagaaacc acgaccttac ccttgaggcc ggtagcggcc
1561 acagtgggtt cgtagatggc agctggagga gacatgtttc aagttgcaat gactatcatc
1621 tgttagccat tccatcaaca ggaagaacga gagaaggcat gacccttttc gctggtatta
1681 tccagatcaa gttttagccg tataatctca gaacgaaccc agtccatcga tgccatgtcc
1741 ttctagacta ggatcctaga gtctagggcc cagcttaggg agggcatgtg aatgcatcga
1801 tgactgggaa cgaacaccgg cccacgccaa agacgttacc taagatacct tgatcattgt
1861 gagagtccag ccaaaagtat tccatgactt ccatcgtatg ccctctagag ggctaatcga
1921 ggagtgtatt tacattgtcg gttggtttgg gaactataga agatggtcag ttattccaat
1981 caccaaaggt ttatcgaagg gaggaagact tgttcagttt cgtccgagga cttttggaat
2041 tcaaatctga gatagagaat tgtgtgggat gagaggaaaa ggaagaagat ggtggggttc
2101 agaaggaggg gttgagttaa atagcatggg ttgagtcaac gtgataaggg cactataccg
2161 tatagatcag cggcacccga ttctatccgt tccttttgct cctctttagc tttgaccggt
2221 gagccggaca agaaacaagt gaaatcatcc tgacatcggc ggacgatctc ctagctttta
2281 catttcgtta ccaatgggat cccgtaatca attgcccatt gaaaaaggaa gagtatgagt
2341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
2401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
2461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
2521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
2581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
2641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
2701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
2761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
2821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
2881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
2941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
3001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
3061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
3121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
3181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
3241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
3301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
3361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
3421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
3481 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
3541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
3601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
3661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
3721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
3781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
3841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
3901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
3961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
4021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
4081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
4141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
4201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
4261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
4321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
4381 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt
4441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
4501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
4561 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
4621 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
4681 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
4741 gctcctttcg ctttcttccc ttcctttctc gccacgttcg cc
//
