| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET41 EK/LIC, pET 41 EK/LIC |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5947 bp |
| 5' 测序引物: | T7或pGEX-5‘ |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3' |
| 载体标签: | N-GST, N-His, N-EK, C-His |
| 载体抗性: | Kanamycin |
| 备注: |
For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
|
| 产品目录号: | 71071-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-41 EK/LIC载体 pET41 EK/LIC质粒基本信息
pET-41 EK/LIC质粒图谱 pET41 EK/LIC载体图谱和pET-41 EK/LIC载体序列 pET41 EK/LIC质粒序列多克隆位点信息
pET-41 EK/LIC质粒 pET41 EK/LIC载体简介
pET-41 EK/LIC载体是用来快速定向PCR高通量质粒构建的载体。本载体带有N-GST标签和His标签，S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET-41a载体上面含有一个EK蛋白酶切位点，当将目的基因使用载体上面的PshAI限制性内切酶位点插入进去时，可以通过EK蛋白酶将载体上的融合的所有氨基酸序列包括GST标签序列，全部切除掉。
pET-41 EK/LIC质粒序列 pET41 EK/LIC载体序列
LOCUS pET-41 Ek/LIC 5947 bp DNA Circular SYN
DEFINITION pET-41 Ek/LIC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5947
/organism="pET-41 Ek/LIC"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(147..1109)
/label="ORF frame 3"
misc_feature complement(279..293)
/label="EK"
misc_feature complement(324..368)
/label="S15"
misc_feature complement(411..428)
/label="6xHIS"
gene complement(459..1109)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1110..1838
/label="ORF frame 3"
misc_feature complement(1118..1134)
/label="T7_transl_en_RBS"
misc_feature complement(1152..1179)
/label="lacO"
promoter complement(1179..1197)
/label="T7_promoter"
gene 1233..1496
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1285..1304)
/label="pBRrevBam_primer"
misc_feature 1579..2670
/label="lacI"
CDS 1711..2670
/label="ORF frame 1"
misc_feature 3242..3433
/label="ROP"
misc_feature complement(3427..3449)
/label="pGEX_3_primer"
rep_origin complement(3848..4467)
/label="pBR322_origin"
gene 4573..5388
/label="KanR2"
/gene="KanR2"
CDS 4573..5388
/label="ORF frame 1"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa
301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg
361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga
421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca
481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta
541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc
601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga
661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc
721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat
781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct
841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg
901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc
961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc
1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta
1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa
1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg
2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac
2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg
2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga
3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat
4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat
4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat
5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
5941 ttcgcca
//
pET-41 EK/LIC载体图谱 pET41 EK/LIC质粒图谱pdf版和pET-41 EK/LIC载体序列 pET41 EK/LIC质粒序列等相关资料下载
pET-41 EK/LIC质粒 pET41 EK/LIC载体应用举例
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