pCDH-DCX-TurboID-V5-puro

LOCUS      pCDH-DCX-TurboID-V5-puro       9587 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on 
            microtubules (DCX as the microtubule-targeting tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Cao X, Shami Shah A, Sanford EJ, Smolka MB, Baskin JM
  TITLE     Proximity Labeling Reveals Spatial Regulation of the 
            Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor.
  JOURNAL   ACS Chem Biol. 2022 Aug 11. doi: 10.1021/acschembio.2c00527.
  PUBMED    35952650
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2022 Aug 11. doi: 10.1021/acschembio.2c00527."
COMMENT     Sequence Label: pCDH-DCX-TurboID-V5-puro
FEATURES             Location/Qualifiers
     source          1..9587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2323..2332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3493..4449
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4456..4497
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4541..4752
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4765..5033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5060..5659
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5060..5079)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5556..5576
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5666..6254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5719..5739)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6137..6148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6328..6561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6633..6754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6670..6689)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6724..6743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6794..6929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6962..6978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6962..6978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6975..6997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6986..7002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7010..7016,7017..7034,7035..7040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7055..7076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7193..7210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7364..7952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8123..8914,8915..8983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8984..9088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9156..9174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9212..9234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9334..9353
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9547..9569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9561..9578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9562..9578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgaattcga atttaaatgg atccgcggcc gcgccaccat ggaacttgat
     2341 tttggacact ttgacgaaag agataagaca tccaggaaca tgcgaggctc ccggatgaat
     2401 gggttgccta gccccactca cagcgcccac tgtagcttct accgaaccag aaccttgcag
     2461 gcactgagta atgagaagaa agccaagaag gtacgtttct accgcaatgg ggaccgctac
     2521 ttcaagggga ttgtgtacgc tgtgtcctct gaccgttttc gcagctttga cgccttgctg
     2581 gctgacctga cgcgatctct gtccgacaac atcaacctgc ctcagggagt gcgttacatt
     2641 tacaccattg atggatccag gaagatcgga agcatggatg aactggagga aggggaaagc
     2701 tatgtctgtt cctcagacaa cttctttaaa aaggtggagt acaccaagaa tgtcaatccc
     2761 aactggtctg tcaacgtaaa aacatctgcc aatatgaaag ccccccagtc tttggctagc
     2821 agcaacagtg cacaggccag ggagaacaag gactttgtgc gccccaagct ggttaccatc
     2881 atccgcagtg gggtgaagcc tcggaaggct gtgcgtgtgc ttctgaacaa gaagacagcc
     2941 cactcttttg agcaagtcct cactgatatc acagaagcca tcaaactgga gaccggggtt
     3001 gtcaaaaaac tctacactct ggatggaaaa caggtaactt gtctccatga tttctttggt
     3061 gatgatgatg tgtttattgc ctgtggtcct gaaaaatttc gctatgctca ggatgatttt
     3121 tctctggatg aaaatgaatg ccgagtcatg aagggaaacc catcagccac agctggccca
     3181 aaggcatccc caacacctca gaagacttca gccaagagcc ctggtcctat gcgccgaagc
     3241 aagtctccag ctgactcagg taacgaccaa gacgcaaacg gaacctccag cagccagctc
     3301 tctaccccca agtctaagca gtctcccatc tctacgccca ccagtcctgg cagcctccgg
     3361 aagcacaagg acctgtacct gcctctgtcc ttggatgact cggactcgct tggtgattcc
     3421 atgagcggcg gccgcggatc aggaagcgga tcaggaagcg gatcaggaag cggatcagga
     3481 agcggatcag gaaaagacaa tactgtgcct ctgaagctga tcgctctcct ggctaatggc
     3541 gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc cgctatcaac
     3601 aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc
     3661 tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg acagctggac
     3721 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     3781 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     3841 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     3901 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     3961 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     4021 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct
     4081 gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg
     4141 gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg
     4201 gccgctacgc tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg
     4261 gctccatatc tgccacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg
     4321 atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg
     4381 ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct
     4441 gccgaaaagg ctagcggcaa gcccatcccc aaccccctgc tgggcctgga cagcacctaa
     4501 gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     4561 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     4621 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4681 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     4741 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     4801 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     4861 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     4921 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     4981 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5041 tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5101 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5161 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5221 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5281 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5341 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5401 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5461 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5521 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5581 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5641 ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     5701 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5761 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5821 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5881 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5941 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6001 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6061 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6121 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6181 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6241 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     6301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag
     6361 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6541 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6601 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6661 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6721 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     6781 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6841 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6901 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca
     6961 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9361 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9421 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9481 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9541 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//