| 质粒名称: | pAAV-hSyn-Cepheid1s-WPRE |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000362 |
| 产品用途: | Expresses Cepheid1s in neuron |
| 存储实验室: | Peng Zou Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Cepheid1s |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets
Han Y., Yang J., Li Y., Chen Y., Ren H., Ding R., Qian W., Ren K., Xie B., Deng M., Xiao Y., Chu J., Zou P.
Sci. Adv. 9, (2023)
|
买家导航
pAAV-hSyn-Cepheid1s-WPRE载体质粒基本信息
pAAV-hSyn-Cepheid1s-WPRE质粒图谱载体图谱和pAAV-hSyn-Cepheid1s-WPRE载体序列质粒序列多克隆位点信息
pAAV-hSyn-Cepheid1s-WPRE质粒载体简介
pAAV-hSyn-Cepheid1s-WPRE质粒序列载体序列
LOCUS pAAV-hSyn-Cepheid1s-WPRE 7224 bp DNA circular SYN 09-DEC-2024
DEFINITION Expression of SomArchon under Syn promoter.
ACCESSION .
VERSION .
KEYWORDS pAAV-Syn-SomArchon
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7224)
TITLE Population imaging of neural activity in awake behaving mice in
multiple brain regions
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7224)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..7224
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1011..1700
/mol_type="genomic DNA"
/organism="unspecified"
source 1011..1700
/mol_type="other DNA"
/organism="pBudCE4.1"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 341..788
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
primer_bind 776..818
/label=hsyn-Ace-F
primer_bind complement(781..800)
/label=pAAV-hsyn-R
regulatory 801..806
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 807..3329
/codon_start=1
/label=Cepheid1s
/translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
IAICTIAATAYYAMAVNYQDLTMLIKEEMPMKVVMTGTVNGHYFKCTGEGEGRPYEGVQ
TMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYED
GGVVTVTQDTSLQDGVLIYNVKVRGENFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGY
TDIALKVDGGGHLHCSFVTEYKSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREV
AVAKYSNLGGGMDELYKNGERQVVYARYICWVLTTPLLLLDLIVMTKMGGVMISWVIGA
DIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEYHSSYVS
LLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAHETIFKT
GAADRPVVAVSKAAAASKSRITSEGEYIPLDQIDINVGGKSRITSEGEYIPLDQIDINV
GGKSRITSEGEYIPLDQIDINVGGNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPY
EGFQTAKLKVTKGGPLPFAWDILSPHFTFGSKAYVKHPADIPDYFKLSFPEGFKWERVM
NYEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA
LKGKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYE
RAEGRHSTGGMDELYKQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDM
RSMSSIDSFISCATDFPEATRFFCYENEV"
primer_bind 883..904
/label=Cepheid-SEQ-2
primer_bind 986..1037
/label=ECL1-mRuby4-F
primer_bind complement(1680..1725)
/label=mRuby3/4-ECL1-R
primer_bind 1785..1803
/label=Cepheid-SEQ-3
primer_bind complement(1961..1982)
/label=Primer 2
primer_bind complement(2197..2218)
/label=Primer 1
primer_bind complement(2403..2423)
/label=sequencing0105_HY
primer_bind 2405..2429
/label=pAAV-TS-F
primer_bind complement(2408..2429)
/label=TS-mO2-R
primer_bind 2681..2699
/label=Cepheid-SEQ-4
primer_bind complement(3093..3113)
/label=mRuby3/4-ECL1-R
misc_feature 3357..3945
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind 3589..3604
/label=Cepheid-SEQ-5
CDS complement(3828..3839)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3965..4441
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind 4471..4484
/label=Cepheid-SEQ-6
repeat_region 4487..4627
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 4487..4616
/label=AAV2 ITR
rep_origin 4702..5157
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 5377..5413
/label=Cepheid-SEQ-7
promoter 5439..5543
/gene="bla"
/label=AmpR promoter
CDS 5544..6404
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6294..6318
/label=Cepheid-SEQ-8
rep_origin 6575..7163
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7204..7224
/label=Cepheid-SEQ-1
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
781 gagcgcagtc gagaggatcc gccaccatgg ctgacgtgga aaccgagacc ggcatgattg
841 cacagtggat tgtctttgct attatggctg ctgctgctat tgcttttgga gtggctgtgc
901 actttcggcc ttcagagctg aagagcgcat actatatcaa cattgccatc tgcactatcg
961 ccgctaccgc ttactatgca atggccgtga actaccagga cctgacaatg ctgatcaagg
1021 aagagatgcc catgaaggtg gtcatgacag gtaccgtcaa cggccactac ttcaagtgca
1081 caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg
1141 agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg tatggcagcc
1201 gtacctttat caagtacccg gccgacatcc ctgatttctt taaacagtcc tttcctgagg
1261 gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc gtcacgcagg
1321 acaccagcct tcaggatggc gtgctcatct acaacgtcaa ggtcagaggg gagaactttc
1381 cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat acagagatga
1441 tgtatccagc agatggtggt ctgagaggat acactgacat cgcactgaaa gttgatggtg
1501 gtggccatct gcactgcagc ttcgtgacag aatacaagtc aaaaaagacc gtcgggaaca
1561 tcaagatgcc cggtgtccat gccgttgatc accgcctgga aaggatcgag gagagtgaca
1621 atgaaaccta cgtagtgcaa agagaagtgg cagttgccaa atacagcaac cttggtggtg
1681 gcatggacga gctgtacaag aatggtgaaa ggcaggtggt ctacgcaaga tatatttgtt
1741 gggtgctgac cacaccactg ctcctgctcg atctcatcgt catgaccaag atgggcggag
1801 tgatgatttc ttgggtcatc ggcgcagaca ttttcatgat cgtgtttggt attctgggcg
1861 ccttcgagga tgaacacaag ttcaaatggg tgtactttat cgctggatgt gtgatgcagg
1921 cagtcctgac atacgggatg tataacgcca cttggaaaga cgatctgaag aaaagccccg
1981 agtaccatag ctcctatgtc agtctgctcg tcttcctgtc aatcctctgg gtgttttatc
2041 ctgtcgtgtg ggctttcggg tctggtagtg gcgtgctgtc cgtcgacaat gaggccattc
2101 tcatgggaat cctggatgtg ctcgctaagc cactgtttgg aatggggtgc ctcattgccc
2161 atgagactat cttcaagacc ggtgccgccg accgcccggt agtagcagtg agcaaggcgg
2221 ccgccgctag caaatcaagg atcacctctg agggcgagta tatccctctg gatcagatcg
2281 acattaacgt cggtggaaaa tcccgcataa cctctgaggg cgagtacatc cctctggatc
2341 aaatcgatat aaacgtgggg ggaaaaagtc gaatcacttc tgagggcgag tatatacccc
2401 tcgatcagat tgacatcaat gtggggggca acatggccat catcaaggag ttcatgcgct
2461 tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
2521 agggccgccc ctacgagggc tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc
2581 tgcccttcgc ctgggacatc ctgtcccctc atttcacctt tggctccaag gcctacgtga
2641 agcaccccgc cgacatcccc gactacttca agctgtcctt ccccgagggc ttcaagtggg
2701 agcgcgtgat gaactacgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
2761 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
2821 ccgtgatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
2881 acggtgccct gaagggcaag atcaagatga ggctgaagct gaaggacggc ggccactaca
2941 cctccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
3001 tcgtcgacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
3061 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagcagagcc
3121 agcctatcct gaacaccaag gagatggccc ctcagagcaa gcctcctgag gagctggaga
3181 tgagcagcat gcctagccct gtggcccctc tgcctgccag gaccgagggc gtgatcgaca
3241 tgaggagcat gagcagcatc gacagcttca tcagctgcgc caccgacttc cctgaggcca
3301 ccaggttctt ctgctatgaa aatgaagtgt aagaattcga tatcaagctt atcgataatc
3361 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
3421 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
3481 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
3541 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
3601 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
3661 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
3721 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
3781 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
3841 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
3901 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccggctcg
3961 agacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
4021 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
4081 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
4141 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
4201 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
4261 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
4321 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
4381 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
4441 tctcgagctg attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg
4501 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
4561 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
4621 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
4681 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
4741 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
4801 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
4861 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
4921 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4981 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
5041 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
5101 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
5161 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
5221 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
5281 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
5341 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
5401 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
5521 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
5581 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
5641 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
5701 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
5761 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
5821 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
5881 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
5941 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
6001 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
6061 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
6121 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
6181 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
6241 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
6301 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
6361 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
6421 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
6481 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
6721 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
7201 tttgctggcc ttttgctcac atgt
//
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