| 质粒名称: | pHCMV_DUGV_GP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 207268 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pHCMV |
| 载体出品公司: | Clontech |
| 空载体大小: | 4764bp |
| 完整质粒大小: | 9417bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | DUGV_GP |
| 物种来源: | Dugbe virus |
| 插入大小: | 4653bp |
| 基因突变: | mammalian codon optimization |
| GenBank编号 | JN247409 |
| 启动子: | CMV promoter |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (not destroyed) |
| 3’克隆位点: | BglII (not destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pHCMV_DUGV_GP was a gift from Feng Zhang (Addgene plasmid # 207268 ; http://n2t.net/addgene:207268 ; RRID:Addgene_207268) |
| 参考文献部分: | Cell type-specific delivery by modular envelope design. Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F. Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. 10.1038/s41467-023-40788-8 PubMed 37612276 |
买家导航
pHCMV_DUGV_GP载体质粒基本信息
pHCMV_DUGV_GP质粒图谱载体图谱和pHCMV_DUGV_GP载体序列质粒序列多克隆位点信息
pHCMV_DUGV_GP质粒载体简介
pHCMV_DUGV_GP质粒序列载体序列
LOCUS pHCMV_DUGV_GP 9417 bp DNA circular SYN 14-APR-2025
DEFINITION Mammalian expression of Dugbe virus GP protein.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9417)
AUTHORS Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
TITLE Cell type-specific delivery by modular envelope design.
JOURNAL Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
PUBMED 37612276
REFERENCE 2 (bases 1 to 9417)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: "
10.1038/s41467-023-40788-8"
COMMENT Sequence Label: pHCMV_DUGV_GP
FEATURES Location/Qualifiers
source 1..9417
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 97..476
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 477..680
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 631..651
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 677..701
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 792..1364
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(6122..6141)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 6187..6242
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(6241..6260)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(6600..6616)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6600..6616)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6613..6635)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6624..6640
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(6648..6654,6655..6672,6673..6678))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6693..6714
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6831..6848)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7002..7590)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7082..7101)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(7761..8552,8553..8621))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8384..8403
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8622..8726)
/gene="bla"
/label=AmpR promoter
rep_origin complement(8752..9207)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8889..8910)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9101..9120
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9334..9356
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9348..9365
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9349..9365
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9375..9394
/label=T7
/note="T7 promoter, forward primer"
promoter 9375..9393
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgagc aaaagggtgc
1441 tcattattgc cgtcgtcgtg tatctggtgt tcacaaccca gaaccagatc accggcaatc
1501 acacaacaat taattctagc agccccagca caacagaagc cagctccacc cctacagtgt
1561 ctagaacccc ccagaccacc accacatcca cagccgtgtc cacaacaatt accgctacaa
1621 ccacccctac cgctagctgg accacacaga gccaatactt caacaaaaca acccagcacc
1681 attggagaga agagaccatg atctctagaa atcccaccgt gctggacaga cagagcagag
1741 ctagcagcgt gagagaactg ctgaacacca aattcctcat gctcctcggc tttatcccca
1801 aaggcgaggt caaccacctc gaaaacgctt gcaatagaga gggaaaaaac tgtacagaac
1861 tgattctgaa agaaaggatc gctagattct tcagcgaaac cgaaaaagaa agctgctaca
1921 acacatatct ggaaaagcat ctgaggagcg tgagccccga agtgagcctc acaccttata
1981 gagtgctggg actgagagag gacattctcc tcaaggagat tgacagaaga atcattagat
2041 tcgaaaccga ctcccagaga gtcacatgcc tctccgcttc tctgctgaag cccgacgtgt
2101 ttattagaga gcagagaatc gacgctaaac cctccaacgg acccaaaatc gtccccgtgg
2161 actccgtggc ttgcatgaat ctcgaggcta acgtggacgt gaggtccaac aaactggtca
2221 tccagagcct catgaccacc gtgaaaatta gcctcaagaa ctgcaaggtg gtggtgaata
2281 gcagacagtg catccatcag caaaccggat ccggcgtgat taaggtgcct aaattcgaaa
2341 agcagcaagg cggaacatgg tcctcctaca ttgctggcgt gtacacagcc accatcgatc
2401 tgctggacga gaacaaccag aactgcaagc tgttcaccga gtgcatcgtg aagggcagag
2461 aactggtgaa gggccagtcc gaactcaaga gcttcaacat tgaagtgctc ctccctaggg
2521 tgatgaaaac cagaaggaag ctcctcgctg tcaccgacgg aagcacagag tgtaacagcg
2581 gcacccaact catcgaagga aagtccatcg aagtgcataa gcaagacatc ggcggacccg
2641 gcaagaaact gaccatctgc aacggcacct ccgtcctcga cgtgcctctc gatgaaggcc
2701 acggatgtta caccatcaac gtgatcacat ccaagagagc ttgcagacct aagaactcca
2761 agctccaatg ctccatcgac aaggagctca aaccttgcga tagcggcaaa tgcctctcca
2821 tctcccaaaa gggagctggc cacatcaagg tgagcagagg caagaccatt ctgattacag
2881 aatgtaaaga gcactgccaa atccccgtgc ccaccggaaa aggagatatt atggtggatt
2941 gttccggcgg cagacagcac tacctcgagg tcaatatcgt ggacatccat tgccccaaca
3001 ccaagtttct gggaggcatc atgctgtact tttgtaggat gagcagcaga cctaccgtcg
3061 ctctgctgct gggcatttgg attggatgcg gctacatcct cacatgcatc ttcagctttc
3121 tgctgtatca cctcattctg tttttcgcta actgtattaa gcaatgtaga aagaagggcg
3181 agaggctggg cgaaatctgt gtcaagtgcg agcaacaaac cgtgaacctc atggatcaag
3241 agctgcacga tctgaattgt aatttcaatc tgtgtcccta ctgttgcaat aggatgagcg
3301 acgaaggaat gtctagacac gtgggcaagt gccccaaaag actcgaaaga ctgaacgaga
3361 ttgaactcta tctgaccacc tccgagtgtc tctgcctcag cgtctgttac cagctgctga
3421 ttagcgtcgg catttttctg aagagaacaa catggctcgt cgtgctcctc gtgctgctgg
3481 gcctcgccat cagccccgtg caaggcgctc ctacagaggt cagcaatgtc aagcaagacg
3541 gcgactacag catttgctac tttatcttcg gatgtctcgt gaccgctgct ctgctgctga
3601 aggtcaagag gaccaacagc aatggaattg tggtggtcgt ggacagcttc ggcagatgcc
3661 cttattgcaa cgagttcacc gactctctgt tcgaggaggt cctccacgac acactctgtt
3721 ccctctgcgt gtgccccttt tgcgagaaac aagccctcga tctggtcaca ctggaagagc
3781 acgtcaagga gtgctacaaa gtcgccacaa ggaaggacat ttttaagatt ctgggaagaa
3841 agttcaccaa tgctctcgtg aggagggaaa aactgttcac caccggactg cagctcttta
3901 tcaataaaac aaacgtggtc gtgttcgctc tgatcatgtg ctttctgctg ctgctgaccg
3961 gccacaacgc tagcgccttt gattccggcg atctgcccga cggcgtgtgg gaagagtcct
4021 cccagctggt caagagctgc acccagttct gctacatcga ggaggacgtc tgttattgcc
4081 ccgccgagga cggcgtggga agaaagctgc tctttttcaa cggactgcaa aacagcgtca
4141 agaggctcag cgattcccac aaactgctca caagcgtcag catcgacgcc ccttggggca
4201 gaattaatgt ggagagcaca tggaagccca cactggccgc ttccaacatc gctatgtctt
4261 ggagcagcac cgatattaaa ggagagaagg tgattctgtc cggaagatcc accagcatca
4321 tcaaactcaa agaaaagacc ggcgtgatgt ggaaactcgt cggcagcggc ctcgcttccg
4381 agaagaagaa acccttcaga ttccctatca tggacttcgc ccaagtgtac aacagcgtgt
4441 tccaatacat caccggcgat agactgctca gcgagtggcc caaagccgtg tgcaccggcg
4501 actgtcctca cagatgcggc tgtcagacaa gcacatgcat ggccaaagaa tgccacaccc
4561 aagagtgcgt gagcacccat atggtgctgg gcatcggcac cggctgcaca tgctgtggaa
4621 tggatgtgga aaggcccttc aacaagtatc tgggcgtcaa gtggtccaca gagtatctga
4681 gaaccgaagt gctcgtgtgt gtggaagtga ccgaggagga gaggcactgt gagatcgtcg
4741 aggctggaac aagatttaac atcggcccta tcaccatcac catcagcgac ccccagaata
4801 ttggctccaa gctccccgaa agcctcatga cagtccaaga gatcgacgac tccaacttcg
4861 tcgacatcat gcacgtggga aacgtgatct ccgccgacaa cagctgtaga ctgcagagct
4921 gcacccatgg atccgctgtg acaaccagat ttacagctct gaccgctctg atcaaagacg
4981 accactcctc cggactgaac ctcgccgtgc tggatcccaa agtcaatagc agctggctca
5041 gctgggaggg ctgcgacatg gactactatt gtaatgtggg agactggccc acatgcacat
5101 ataccggcgt cgtcacccaa aagctgagag agttcctcaa gctcgaccag catagaaaga
5161 ggctgcacac caccctcagc ttttctctga agaagaatct gtccaaaagg tcccacacat
5221 ccgtgagact cgagggcaaa acagtgacaa gaatggaggt gaaggtcaca gctctgattg
5281 aagtggacgg catggagctc catagcaaga ccattagact cagcggaatc agactgaccg
5341 gcctcaagtg cagcggctgt tttagctgca caagcggcat tagctgttcc gtgaacgcca
5401 agctgacaag ccccgacgag tttacactgc acctcagatc cacatcccct aatgtggtcg
5461 tggccgagac cagcattatc gccagaaaag gcccctccgc caccacaagc agattcaagg
5521 tgttcagcgt gagggatacc aagaaaatct gcttcgaagt ggtggagagg gagtactgca
5581 aagactgcac acccgacgaa ctgacaacat gcaccggcgt cgagctcgaa cctaccaagg
5641 atattctgct ggaacatagg ggcaccatcg tccagcacca gaacgataca tgcaaatcca
5701 aaatcgattg ttggagcaat tccatttcca gctttgcctc cggcatcggc gactttttta
5761 agcattacat cggctccatc gctgtgggag tgctgggaac cgtgctcccc tttgccctcc
5821 tcattctctt tttcatctat ggcgacaaga tgctctggcc cttcaaagtg ttctgtaggc
5881 cttgtagaag atgctgtagg aagaacgaag gctacaacaa gctcgctgag gaggaggagc
5941 tgagagatat cattaggaag tttagcaagt ccggagaact gattaacaag gacgccaagg
6001 acaaaaggac actggctaga ctcttcatgt ccgataaccc taagctgaag aaggagaaaa
6061 agctcagcga gatcgcctga tgtcttcctg gagctccagt cattgactat aacgtctcag
6121 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
6181 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
6241 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
6301 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
6361 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
6421 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
6481 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
6541 tagctgtccc tcttctctta tggagatccc tcgacggatc ggccgcaatt cgtaatcatg
6601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
6661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
6721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
6781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
6841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
6901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
6961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
7021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
7081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
7141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
7201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
7261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
7321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
7381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
7441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
7501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
7561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
7621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
7681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
7741 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
7801 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
7861 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggca
7921 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
7981 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
8041 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
8101 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
8161 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
8221 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
8281 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
8341 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
8401 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
8461 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
8521 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
8581 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
8641 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
8701 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
8761 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
8821 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
8881 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
8941 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
9001 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
9061 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
9121 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
9181 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
9241 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
9301 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
9361 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtg
//
pHCMV_DUGV_GP载体图谱质粒图谱pdf版和pHCMV_DUGV_GP载体序列质粒序列等相关资料下载
pHCMV_DUGV_GP质粒载体应用举例
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