| 质粒名称: | pDriver |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165149 |
| 存储实验室: | Paul Khavari |
|
载体骨架基本信息 |
|
| 载体名称: | pUC-like |
| 载体出品公司: | Clontech |
| 空载体大小: | 5302bp |
| 完整质粒大小: | 5302 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SV40 Large T-antigen、SVLT |
|
文献引用方法 |
|
| 材料和方法部分: | pDriver was a gift from Paul Khavari (Addgene plasmid # 165149 ; http://n2t.net/addgene:165149 ; RRID:Addgene_165149) |
| 参考文献部分: | PROBER identifies proteins associated with programmable sequence-specific DNA in living cells. Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V, Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y, Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA. Nat Methods. 2022 Aug;19(8):959-968. doi: 10.1038/s41592-022-01552-w. Epub 2022 Aug 4. 10.1038/s41592-022-01552-w PubMed 35927480 |
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pDriver载体质粒基本信息
pDriver质粒图谱载体图谱和pDriver载体序列质粒序列多克隆位点信息
pDriver质粒载体简介
pDriver质粒序列载体序列
如LOCUS pDriver 5302 bp DNA circular SYN 14-APR-2025
DEFINITION Driver plasmid for PROBER encoding SV40 Large T-antigen.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5302)
AUTHORS Mondal S, Ramanathan M, Miao W, Meyers RM, Rao D, Lopez-Pajares V,
Siprashvili Z, Reynolds DL, Porter DF, Ferguson I, Neela P, Zhao Y,
Meservey LM, Guo M, Yang YY, Li L, Wang Y, Khavari PA
TITLE PROBER identifies proteins associated with programmable
sequence-specific DNA in living cells.
JOURNAL Nat Methods. 2022 Aug;19(8):959-968. doi:
10.1038/s41592-022-01552-w. Epub 2022 Aug 4.
PUBMED 35927480
REFERENCE 2 (bases 1 to 5302)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41592-022-01552-w"; journalName: "Nat Methods"; date:
"2022-08"; volume: "19"; issue: "8"; pages: "959-968"
COMMENT Sequence Label: pDriver
FEATURES Location/Qualifiers
source 1..5302
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 505..529
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 594..2729
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKFSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGVGVLDWLRNSDDD
DDDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQPSQSSQSVHDHNQPYHICR
GFTCFKKPSPPPPEPET"
primer_bind complement(2761..2778)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2767..2878
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3037..3172
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3099..3118
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3230..3247)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3401..3989)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3481..3500)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4160..4951,4952..5020))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4783..4802
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5021..5125)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tccatggata
601 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
661 cctgggggaa tattcctctg atgagaaagg catatttaaa aaaatgcaag gagtttcatc
721 ctgataaagg aggagatgaa gaaaaaatga agaaaatgaa tactctgtac aagaaaatgg
781 aagatggagt aaaatatgct catcaacctg actttggagg cttctgggat gcaactgaga
841 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
901 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
961 ctactcctcc aaaaaagaag agaaaggtag aagatcccaa ggactttcct tcagaattgc
1021 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
1081 ccacaaagga aaaagctgca ctgctataca aaaaaattat ggaaaaattt tctgtaacct
1141 ttataagtag gcataacagt tacaatcata acatactgtt ttttcttact ccacacaggc
1201 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
1261 gtaaaggggt taataaggaa tatttgatgt atagtgcctt aactagagat ccattttctg
1321 ttattgagga aagtttgcct ggtgggttaa aggagcatga ttttaatcca gaagaagcag
1381 aggaaactaa acaagtgtcc tggaagcttg taacagagta tgcaatggaa acaaaatgtg
1441 atgatgtgtt gttattgctt gggatgtact tggaatttca gtacagtttt gaaatgtgtt
1501 taaaatgtat taaaaaagaa cagcccagcc actataagta ccatgaaaag cattatgcaa
1561 atgctgctat atttgctgac agcaaaaacc aaaaaaccat atgccaacag gctgttgata
1621 ctgttttagc taaaaagcgg gttgatagcc tacaactaac tagagaacaa atgttaacaa
1681 acagatttaa tgatcttttg gataggatgg atataatgtt tggttctaca ggctctgctg
1741 acatagaaga atggatggct ggagttgctt ggctacactg tttgttgccc aaaatggatt
1801 cagtggtgta tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa agatactggc
1861 tgtttaaagg accaattgat agtggtaaaa ctacattagc agctgctttg cttgaattat
1921 gtggggggaa agctttaaat gttaatttgc ccttggacag gctgaacttt gagctaggag
1981 tagctattga ccagttttta gtagtttttg aggatgtaaa gggcactgga ggggagtcca
2041 gagatttgcc ttcaggtcag ggaattaata acctggacaa tttaagggat tatttggatg
2101 gcagtgttaa ggtaaactta gaaaagaaac acctaaataa aagaactcaa atatttcccc
2161 ctggaatagt caccatgaat gagtacagtg tgcctaaaac actgcaggcc agatttgtaa
2221 aacaaataga ttttaggccc aaagattatt taaagcattg cctggaacgc agtgagtttt
2281 tgttagaaaa gaggataatt caaagtggca ttgctttgct tcttatgtta atttggtaca
2341 gacctgtggc tgagtttgct caaagtattc agagcagaat tgtggagtgg aaagagagat
2401 tggacaaaga gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg gctatgggag
2461 ttggagtttt agattggcta agaaacagtg atgatgatga tgatgacagc caggaaaatg
2521 ctgataaaaa tgaagatggt ggggagaaga acatggagga ctcagggcat gaaacaggca
2581 ttgattcaca gtctcaaggc tcatttcagg cccctcagcc ctcacagtcc tcacagtctg
2641 ttcatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctt
2701 ccccacctcc ccctgaacct gaaacataag aattccctgc tctcaaaact gtcttctcag
2761 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
2821 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcga
2881 agacagtcag ccttgtggaa tgtgtgtcag ttagagtagt tcatgtcatc ttattattca
2941 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgtttaaa
3001 ccgtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
3061 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
3121 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
3181 tgcaaaaagc tcggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
3241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
3301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
3361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
3421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
3481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
3541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
3601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
3661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
3721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
3781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
3841 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
3901 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
3961 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
4021 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
4081 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
4141 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
4201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
4261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
4321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
4381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
4441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
4501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
4561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
4621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
4681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
4741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
4801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
4861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
4921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
4981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
5041 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
5101 aaaataaaca aataggggtt ccgcgcacat ttcccctaaa agtgccacct gacgtcgacg
5161 gatcgggaga tctctagaga tccgactcga gtactaggat ccattaggcg gccgcgtgga
5221 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
5281 atagcccata tatggagttc cg
//
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