| 质粒名称: | pGEX-6p-1-AT5G48650 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000924 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | AT5G48650 |
| 物种来源: | Arabidopsis thaliana |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-AT5G48650载体质粒基本信息
pGEX-6p-1-AT5G48650质粒图谱载体图谱和pGEX-6p-1-AT5G48650载体序列质粒序列多克隆位点信息
pGEX-6p-1-AT5G48650质粒载体简介
pGEX-6p-1-AT5G48650质粒序列载体序列
LOCUS pGEX-6p-1-AT5G48650 6370 bp DNA circular SYN 09-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6370)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6370
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 978..2351
/codon_start=1
/label=AT5G3BP7(48650)
/translation="MDSTAATKRVVDPLTVGSAFVNQYYYIFCNMPEHLPRFYQEISRV
GRVGQDGVMRDFSTFQGISEELKRLTYGDCNSAEITSYDTQESHNGGFLLFVTGYFTLN
ERSRRKFTQTFFLAPQEKGFFVLNDILRFVNDDAKDNVPETIDGEVVSGINSTTPTIIN
GMKGSEQAACVSVNPVCKEVSKPLDNENAKDNVLVPEIANEVARTEITCKEVADDSQKN
YDPDDGLADAPKKSYASVLKVTKDKFGVPAVSLPSPKKIPKDQEHQAPSDPSTGQILKD
QGQQASSDPSQVIESDTVSESVDASENGHNQEAVAEGTSIYVRHLPFNANIDMLEAEFK
QFGAITNGGIQVINQRGLGYPYGFVEFEEADAAHRAIEASPVKIGGLRAFVEEKLSTSR
GKRGNGNVGYGNRNVGVGMRGRGSYGYGYDYRRGGRGPGGGGRSFNRRGNEYVASINSY
"
promoter 2673..2777
/gene="bla"
/label=AmpR promoter
CDS 2778..3638
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3809..4397
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4641..4718
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4719..5801
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5814..5835
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5850..5880
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5888..5904
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5912..5928
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5924..6370
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5944..5960)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggtagtatg gatagtaccg cagcaaccaa acgcgttgtt gatccgctga
1021 ccgtgggtag cgcatttgtt aatcagtatt attatatctt ctgcaacatg ccggaacatc
1081 tgccgcgctt ttatcaggaa attagtcgtg tgggtcgtgt tggtcaggat ggtgttatgc
1141 gcgattttag cacctttcag ggcattagcg aagaactgaa acgtctgacc tatggtgact
1201 gtaatagtgc agaaattacc agctatgata cccaggaaag ccataatggc ggttttctgc
1261 tgtttgttac cggctatttt accctgaatg aacgtagtcg ccgcaaattc actcagacct
1321 ttttcctggc cccgcaggaa aaaggtttct ttgtgctgaa tgatattctg cgctttgtta
1381 atgatgatgc aaaagataat gtgccggaaa ccattgatgg tgaagttgtg agtggtatta
1441 atagcaccac cccgaccatt attaatggca tgaaaggcag tgaacaggca gcatgtgtga
1501 gcgtgaatcc ggtgtgtaaa gaagttagta aaccgctgga taatgaaaat gcaaaagata
1561 acgttctggt tccggaaatt gcaaatgaag tggcacgcac cgaaattacc tgtaaagaag
1621 tggcagatga tagtcagaaa aattatgatc cggatgatgg cctggccgat gcaccgaaaa
1681 aatcttatgc aagcgttctg aaagtgacca aagataaatt tggcgttccg gccgtgagtc
1741 tgccgagccc taaaaagatt ccgaaagatc aggaacatca ggcaccgagt gatccgagca
1801 ccggtcagat tctgaaagat cagggtcagc aggccagtag tgatccgagt caggttattg
1861 aaagcgatac cgttagcgaa agcgttgatg ccagtgaaaa tggtcataat caggaagcag
1921 tggcagaagg tacaagcatc tatgttcgcc atctgccgtt taatgccaat attgatatgc
1981 tggaagcaga gtttaaacag tttggcgcaa ttaccaatgg cggtattcag gttattaatc
2041 agcgcggcct gggttatccg tatggttttg ttgaatttga agaagccgat gcagcacatc
2101 gtgccattga agcaagtccg gtgaaaattg gtggtctgcg cgcatttgtt gaagaaaaac
2161 tgagtaccag ccgtggcaaa cgcggcaatg gcaatgttgg ctatggcaat cgcaatgtgg
2221 gcgtgggtat gcgtggtcgc ggcagctatg gttatggtta tgattatcgc cgtggtggcc
2281 gcggccctgg tggtggtggt agaagtttta atcgccgtgg taatgaatat gttgcaagta
2341 ttaatagcta ctaactcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
2401 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2461 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2521 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
2581 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
2641 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
2701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
2761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
2821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
2881 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
2941 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
3001 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
3061 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
3121 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
3181 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
3241 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
3301 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
3361 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
3421 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
3481 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
3541 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
3601 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
3661 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
3721 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
3781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
3841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
3901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
3961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
4021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
4081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
4141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
4561 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4621 tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat
4681 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
4741 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
4801 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
4861 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
4921 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
4981 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
5041 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
5101 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
5161 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
5221 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
5281 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
5341 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
5401 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
5461 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
5521 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgtcaacca
5581 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
5641 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
5701 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
5761 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
5821 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
5881 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga
5941 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
6001 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
6061 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
6121 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
6181 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta
6241 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
6301 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
6361 cgttggaatt
//
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