| 质粒名称: | pHisB-Sumo-NeMeHg |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000571 |
| 产品用途: | Expresses NeMeHg in bacterial cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpa, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NeMeHg |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
NeMeHg, genetically encoded indicator for mercury ions based on mNeonGreen green fluorescent protein and merP protein from Shigella flexneri
Subach O.M., Piatkevich K.D., Subach F.V.
Front. Bioeng. Biotechnol. 12, 1407874 (2024)
|
买家导航
pHisB-Sumo-NeMeHg载体质粒基本信息
pHisB-Sumo-NeMeHg质粒图谱载体图谱和pHisB-Sumo-NeMeHg载体序列质粒序列多克隆位点信息
pHisB-Sumo-NeMeHg质粒载体简介
pHisB-Sumo-NeMeHg质粒序列载体序列
LOCUS pHisB-Sumo-NeMeHg 5340 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5340)
AUTHORS 1
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..5340
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 305..310
/label=SDS
misc_feature 319..351
/label=His-tag
/note="/vntifkey=21"
CDS 331..348
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 352..384
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 388..411
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
misc_feature 424..717
/label=Sumo(with deleted BglII site)
/label=Sumo(with\deleted\BglII\site)
/note="/vntifkey=21"
CDS 424..717
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/label=SUMO
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
misc_feature 724..1695
/label=NeMeHg
/note="/vntifkey=21"
terminator 1911..1997
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2089..2116
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 2135..2226
/gene="bla"
/label=AmpR promoter
CDS 2227..3087
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 3088..4028
/label=ColE1
/note="ColE1"
rep_origin 3258..3846
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4032..4172
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4436..5314)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc
361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc
421 tcgatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
481 cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatttt cttcaagatc
541 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
601 atggactcct taagattctt gtacgacggt attagaatcc aagctgatca gacccctgaa
661 gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat tggtggaaga
721 tctatggcta cccagaccgt cacactagcg gttcccggca tgacttgcgc cgcctgcccg
781 atcacagtca agaaagcgct ctccaaggtc gaaggcgtga gcaaggtcga tgtgggcttc
841 gagaatcctg ctgactggcg tatatccaag aaggcttgcc ccaatggtaa gactctcatt
901 tccacactta agtggtctta taccaccggc aacggcaaac gctaccggtc tactgctcgc
961 accacataca cattcgccaa accaatggcc gcaaattacc tcaaaaatca gccgatgtat
1021 gtctttcgga aaacagagct gaaacattct aagaccgaaa tggattttaa agagtggcaa
1081 aaggccttta cggacgtgat gggcatggac gagctgtata aggtggatgg cggcagcggt
1141 ggcaccggca tggtctcaga gggagagggg gacaacatgg ctagtctgcc tgctacccac
1201 gaactgcata tttttggctc cataaatggc gtcgatttcg acatggtagg ccaaggctcc
1261 ggtaatccaa acgtcggtta tgaggagctg aacctgaagt ccaccaaggg cgatctgcag
1321 ttcagtccct ggatcctggt gccgcacatt gggtacgggt ttcatcagta tctcccctac
1381 ccggacggga tgtcaccatt tcaggctgca atggtcgacg ggtccggata tcaggtgcac
1441 cgcactgtgc agtttgagga cggtgcttca ctgaccgtga attacagata cacctacgaa
1501 ggatcacata tcaaagggga agctcaggtg aagggcactg ggttcccggc agacggccct
1561 gtgatgacaa attccctgac agcatttgtt tgtaagcgcg aggtcatcgt cacttttgac
1621 gacaccaagg ccagcgtaca gaagctgacc agggccaccg cagacgccgg ctatccgtcc
1681 agcgtcaagc agtgagaatt cgaagcttgg ctgttttggc ggatgagaga agattttcag
1741 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
1801 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
1861 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
1921 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
1981 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
2041 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
2101 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca
2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
2221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
2281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
2341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
2401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
2461 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
2521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
2581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
2641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
2701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
2761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
2821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
2881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
2941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
3001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
3061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
3121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
3961 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
4021 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
4081 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
4141 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
4201 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4261 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca
4321 attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga
4381 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
4441 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
4501 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
4561 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
4621 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
4681 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
4741 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
4801 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
4861 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
4921 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg
4981 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
5041 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
5101 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
5161 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
5221 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
5281 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
//
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