pPB-AMBRA1-3xFLAG

LOCUS       pPB-AMBRA1-3xFLAG               10964 bp ds-DNA     circular SYN 10-SEP-2021
DEFINITION  PiggyBac vector to stably express 3xFLAG-tagged AMBRA1.
ACCESSION   .
VERSION     .
KEYWORDS    pPB-AMBRA1-3xFLAG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R,
            Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, 
            Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, 
            McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, 
            Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
  TITLE     The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
  JOURNAL   Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7.
            Epub 2021 Apr 14.
  PUBMED    33854239
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        337..1515
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          568..1506
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1463..1483
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1515..1539
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5188..5253
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5257..5281)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5359..5378)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5424..5479
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5478..5497)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        5834..6213
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6214..6417
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6368..6388
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6450..7244
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6504..6523)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7114..7133
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7282..7299)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7288..7512
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7862..7924
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7990..8009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7991..8009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8016..8033)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8016..8032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8025..8047)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8174..8629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8261..8280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8471..8492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8655..8759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8760..9620
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8978..8997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9791..10379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10280..10299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10533..10550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10667..10688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10703..10733
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10741..10757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10746..10768
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10765..10781
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10765..10781
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10802..10820
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgggct ccggtgcccg tcagtgggca
      361 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      421 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      481 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      541 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      601 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      661 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
      721 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
      781 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      841 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
      901 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
      961 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1021 gggtagtctc aagctggccg gcctgctctg gtgcctggtc tcgcgccgcc gtgtatcgcc
     1081 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1141 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1201 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1261 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1321 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1381 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1441 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1501 tttcaggtgt cgtgacaagt ttgtacaaaa aagcaggctg ccaccatgaa ggttgtccca
     1561 gaaaagaatg ctgtccggat actctggggg cgagaacggg gtgctcgggc catgggagct
     1621 cagcggcttc tgcaggagct ggtagaggat aaaacccggt ggatgaaatg ggagggcaag
     1681 agagtagaac tgccggattc accacgctct accttcttat tggccttcag cccagacagg
     1741 actctcttag ccagcaccca tgtgaaccat aatatctata ttacggaggt gaagactggc
     1801 aagtgtgttc attccctgat tggacaccgc cgtactccat ggtgtgtcac ttttcatccc
     1861 accatctcag gccttattgc ttctggctgc ctagatgggg aggttaggat ttgggattta
     1921 cacggtggca gtgaaagctg gttcacagat agcaacaatg ccattgcctc cctggctttc
     1981 caccctacgg ctcagctcct gctgattgcc actgccaatg agatccactt ctgggactgg
     2041 agtcgacggg aaccctttgc tgtggtgaag acagctagtg agatggaacg ggtccgtctg
     2101 gtgagatttg atccacttgg acactactta ctcacagcaa ttgttaaccc ctctaatcaa
     2161 cagggtgatg acgaaccaga gatccccata gatggaacag aattatccca ctaccgtcag
     2221 cgtgccctcc tgcaatcaca gccagttcgc cggacgcctc tcctccacaa tttcctgcac
     2281 atgctgtcct cccgctcttc tggcatccag accgagccct tccatccccc ggagcaggcc
     2341 tcgtcaacgc agcaggacca gggcctcctg aaccggccgt ctgccttcag tacagtccag
     2401 agcagcactg ccggcaacac gctccgcaac ctcagtctgg gtcctacccg ccgctctttg
     2461 ggagggcctc tgtctagcca cccttctagg tatcaccgag aaatagctcc tgggttgaca
     2521 ggatctgagt ggacccggac agtactcagt ctgaactccc gctctgaggc ggaatccatg
     2581 cccccgccca gaaccagtgc ctcttcggtg agtttgctgt ctgtgctgag acagcaggaa
     2641 ggtggctctc aggcatctgt gtacacttca gccacagaag ggaggggttt tccggcatca
     2701 gggttggcaa ctgagtcaga tggagggaat ggctccagcc aaaacaactc gggcagcatt
     2761 cgccatgagc ttcagtgtga cctgagacgc ttctttctgg agtatgaccg gcttcaggag
     2821 ctggatcaga gcctgagtgg ggaagctccc cagacccaac aggcccagga aatgctcaac
     2881 aataacattg aatctgagag gccaggccct tcccaccagc ccaccccaca cagcagtgag
     2941 aacaactcca acctgtcccg tggccacctg aatcgctgtc gtgcttgcca caatctcctg
     3001 accttcaaca acgataccct gcgctgggaa agaaccacac ctaactactc ctctggcgag
     3061 gctagttcct cttggcaggt ccccagctcc tttgagagtg tgccatcaag tggcagccag
     3121 ttgccacctc tcgagcggac tgagggccaa acgcccagct ccagcaggct ggagttgagc
     3181 agctctgcta gtccgcagga ggagaggact gtgggggtgg cctttaacca ggagacaggc
     3241 cactgggaaa gaatttacac ccagtccagc agatctggaa ctgtgtcaca ggaggcctta
     3301 catcaggata tgcctgagga gagctctgag gaggattcac tcaggaggag gctgctggaa
     3361 tcttccctca tttcattatc ccgttatgat ggagcaggat ccagagagca cccaatttac
     3421 ccagacccag cgagattatc tcctgctgca tactacgccc agaggatgat ccagtatctc
     3481 tcacggagag acagtattcg ccagcgctcc atgcgctacc aacagaaccg tctccgttct
     3541 tccacctcct cctcttcctc agacaaccag ggtccatcag tagagggaac cgacttggaa
     3601 tttgaggact ttgaggacaa tggtgacaga tccaggcacc gagctccacg caatgcccgg
     3661 atgtctgcac cttcgcttgg acgctttgtc ccaaggcgtt tcttgctgcc tgagtacttg
     3721 ccttatgctg ggatttttca tgaacgtgga cagcctggct tggctactca ctcttctgtt
     3781 aacagggtcc tggcaggggc agtgatcggt gatggacagt ctgctgtggc cagtaacatt
     3841 gccaatacta cctaccggct ccagtggtgg gacttcacta agtttgacct ccctgaaatc
     3901 agtaatgctt ccgtgaatgt gctggtgcag aactgcaaga tctacaatga tgccagctgt
     3961 gacatttctg cagatggcca gctcctggca gctttcatcc ccagcagcca gaggggcttt
     4021 cctgatgaag gcatcctggc agtgtactcc ctggcccccc ataacctggg cgaaatgctc
     4081 tacaccaagc gatttggtcc caatgccatt tcggtgagcc tgtccccaat gggcagatat
     4141 gtaatggtgg gcttggcctc acgaaggatc ctgctgcacc cctccacaga gcacatggtg
     4201 gcccaggtct tcaggctgca acaggcccat ggtggagaga cctccatgag gagagttttc
     4261 aacgtccttt atcccatgcc tgccgaccag cggagacatg tcagtatcaa ctctgcccgt
     4321 tggctgcctg agccagggct tggcttggcc tatggtacta acaaaggaga cctggtgatc
     4381 tgccgaccag aggccttaaa ctctggtgtt gagtactact gggaccagct gaacgagacg
     4441 gtcttcactg tccattccaa cagcaggagc agcgagcggc ctggaaccag cagagccaca
     4501 tggaggacag acagagacat ggggctgatg aatgccattg ggcttcagcc ccggaaccct
     4561 gccacctcag tgacatctca gggcacccag actctggccc ttcagctgca gaatgccgaa
     4621 acacagactg agagggaggt gccggagcca gggacagccg cctcaggtcc tggtgaaggt
     4681 gagggttcag agtatggtgc cagtggagaa gatgcgctca gcaggatcca gaggctgatg
     4741 gcggagggcg gcatgacagc cgtggtgcag cgggagcaga gcaccaccat ggcctccatg
     4801 ggcggcttcg gcaacaacat catcgtcagc caccgcattc accgcagctc tcagacgggc
     4861 actgaacctg gtgccgccca cacctcctca ccccagccct ccacctctcg gggactgctc
     4921 ccagaggccg ggcaactggc agagcgaggc ctaagccccc ggacagcttc ctgggaccag
     4981 cctggtaccc ctgggcggga gccaacccag ccaaccctgc cctcttcctc ccctgtcccc
     5041 attcctgttt cccttcccag cgctgaggga ccaaccctcc actgcgagtt gaccaataac
     5101 aaccaccttc tggatggtgg cagcagcagg ggggacgctg caggccctag gggagaacca
     5161 cggaacaggg gcagcggcag cggcagcgac tacaaagacc atgacggtga ttataaagat
     5221 catgatatcg attacaagga tgacgatgac aagtagaccc agctttcttg tacaaagtgg
     5281 tgatcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     5341 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     5401 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     5461 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     5521 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     5581 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     5641 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     5701 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     5761 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     5821 caagcttcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     5881 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     5941 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6001 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6061 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6121 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6181 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     6241 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     6301 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     6361 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     6421 tggctaacta gagaacccac tgcgccacca tgattgaaca agatggattg cacgcaggtt
     6481 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6541 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6601 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     6661 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6721 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6781 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6841 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6901 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6961 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7021 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7081 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7141 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7201 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgactcgag tctagagggc
     7261 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7321 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7381 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7441 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7501 tgggctctat ggctcgagtt aattaacgag agcataatat tgatatgtgc caaagttgtt
     7561 tctgactgac taataagtat aatttgtttc tattatgtat aggttaagct aattacttat
     7621 tttataatac aacatgactg tttttaaagt acaaaataag tttatttttg taaaagagag
     7681 aatgtttaaa agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata
     7741 aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat
     7801 aataaaactt aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac
     7861 acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct
     7921 agggttaaat aatagtttct aattttttta ttattcagcc tgctgtcgtg aataccgagc
     7981 tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     8041 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8101 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8161 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8581 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     8641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10621 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10681 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10741 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg
    10801 aaattaaccc tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc
    10861 tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc
    10921 tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc tttt
//