| 载体名称: | pFR-luc,pFRLuc |
|---|---|
| 质粒类型: | 哺乳动物双杂交系统 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5.8 kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
买家导航
pFR-Luc载体 pFRLuc质粒基本信息
pFR-Luc质粒图谱 pFRLuc载体图谱和pFR-Luc载体序列 pFRLuc质粒序列多克隆位点信息
pFR-Luc质粒 pFRLuc载体简介
pFR-Luc质粒序列 pFRLuc载体序列
LOCUS pFR-Luc 5748 bp DNA linear SYN 26-JUL-2016
DEFINITION Cloning vector pFR-Luc, complete sequence.
ACCESSION AF058756
VERSION AF058756.1
KEYWORDS .
SOURCE Cloning vector pFR-Luc
ORGANISM Cloning vector pFR-Luc
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5748)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (09-APR-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 2 (bases 1 to 5748)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (19-OCT-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REMARK Sequence update by submitter
REFERENCE 3 (bases 1 to 5748)
AUTHORS Zheng,C.-F.
TITLE Direct Submission
JOURNAL Submitted (22-JAN-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REMARK Sequence update by submitter
COMMENT On Jan 24, 1999 this sequence version replaced gi:3769433.
FEATURES Location/Qualifiers
source 1..5748
/organism="Cloning vector pFR-Luc"
/mol_type="genomic DNA"
/db_xref="taxon:75696"
/lab_host="Escherichia coli K12"
/plasmid="pFR-Luc"
CDS 137..997
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAD08914.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 998..1930
/note="Col EI origin"
repeat_region 2692..2785
/note="five 17nt GAL4 binding elements"
/rpt_type=tandem
regulatory 2804..2809
/regulatory_class="TATA_box"
CDS 2868..4520
/note="from Photinus pyralis"
/codon_start=1
/transl_table=11
/product="luciferase"
/protein_id="AAD08913.1"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
ORIGIN
1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
2641 actcatcaat gtatcttatc atgtctggat ccaagcttgc atgcctgcag gtcggagtac
2701 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
2761 cctccgagcg gagtactgtc ctccgagcgg agactctaga gggtatataa tggatccccg
2821 ggtaccgagc tcgaattcca gcttggcatt ccggtactgt tggtaaaatg gaagacgcca
2881 aaaacataaa gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc
2941 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
3001 cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag
3061 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
3121 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
3181 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg
3241 tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa
3301 taatccagaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
3361 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt
3421 cctttgatcg tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac
3481 ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc
3541 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
3601 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
3661 tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg
3721 cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg
3781 atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg
3841 aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga
3901 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
3961 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
4021 atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc
4081 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
4141 actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca
4201 aaggatatca ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct
4261 tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
4321 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
4381 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
4441 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
4501 gcggaaagtc caaattgtaa aatgtaactg tattcagcga tgacgaaatt cttagctatt
4561 gtaatactct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
4621 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
4681 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aacttatgaa
4741 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
4801 tctagtgatg atgaggctac tgctgactct caacattcta ctctcaaaag aagagaaagg
4861 tagagaccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
4921 aatagaactc ttgcttgctt tgctatttac aaccacaaag gaaaaagctg cactgctata
4981 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
5041 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
5101 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
5161 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
5221 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
5281 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
5341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
5401 tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
5461 tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
5521 attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
5581 ggtaatttta aaatatctgg gaagtccctt ccactgctgt gttccagaag tgttggtaaa
5641 cagcccacaa atgtcaacag cagaaacata caagctgtca ctttgcacaa agggcctcgt
5701 gatacgccta tttttatagg ttaatgtcat gataataatg gtttctta
//
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