| 出品公司: | Novagen |
|---|---|
| 载体名称: | pIEX-3, pIEX3 |
| 载体又名: |
pIEX-3载体，pIEX-3质粒，pIEX-3载体质粒 pIEX3载体，pIEX3质粒，pIEX3载体质粒 |
| 英文名称: |
pIEX-3, pIEX-3 Vector, pIEX-3 Plasmid pIEX3, pIEX3 Vector, pIEX3 Plasmid |
| 质粒类型: | 昆虫细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | IE1 |
| 载体大小: | 4.6 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | N-GST, N-His, C-HSV |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 昆虫细胞 |
| 备注: | |
| 产品目录号: | 217568 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pIEX-3载体 pIEX3质粒基本信息
pIEX-3质粒图谱 pIEX3载体图谱和pIEX-3载体序列 pIEX3质粒序列多克隆位点信息
pIEX-3质粒 pIEX3载体简介
pIEX-3质粒序列 pIEX3载体序列
pIEX-3 snapgene格式序列 pIEX-3 .gb格式序列
LOCUS pIEx-3 4629 bp DNA circular SYN 30-JUL-2025
DEFINITION Vector for high-level expression in insect cells of secreted
proteins with a cleavable N-terminal GST-6xHis-S-Tag cassette plus a
C-terminal HSV tag.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4629)
AUTHORS MilliporeSigma (Novagen)
TITLE Direct Submission
JOURNAL Exported Jul 30, 2025 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT Sequence Label: pIEx-3
COMMENT VNTDATE|696124800|
COMMENT VNTDBDATE|1100217600|
COMMENT VNTNAME|pIEx(TM)-3|
COMMENT VNTAUTHORNAME|MilliporeSigma (Novagen)|
FEATURES Location/Qualifiers
enhancer 1..483
/gene="Autographa californica hr5"
/label=hr5 enhancer
/note="baculovirus early transcription enhancer"
promoter 487..1078
/label=IE1 promoter
/note="promoter of the ie1 gene from the baculovirus
Autographa californica"
CDS 1084..1143
/codon_start=1
/product="signal sequence from tobacco hornworm
adipokinetic hormone"
/label=AKH signal sequence
CDS 1150..1806
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
CDS 1834..1851
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
CDS 1861..1905
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
CDS 1921..1938
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
CDS 1957..1971
/codon_start=1
/product="enterokinase recognition and cleavage site"
/label=enterokinase site
misc_feature 1966..2080
/label=MCS
CDS 2086..2118
/codon_start=1
/product="HSV (herpes simplex virus) epitope tag"
/label=HSV tag
terminator 2146..2453
/label=IE1 terminator
/note="terminator of the ie1 gene from the baculovirus
Autographa californica"
promoter 2483..2587
/gene="bla"
/label=AmpR promoter
CDS join(2588..2656,2657..3448)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 3619..4207
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 actatgtaca agctcacagt cttcctgatg ttcatcgctt tcgtcatcat cgctgaggcc
1141 cagctgacca tggcatcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc
1201 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat
1261 gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct
1321 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct
1381 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa
1441 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa
1501 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt
1561 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat
1621 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta
1681 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc
1741 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct
1801 ccaaaatcgg atggttcaac tagtggttct ggtcatcacc atcaccatca ctcttctggt
1861 aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg actcgccacc gccttctggt
1921 ctggtccccc ggggcagcgc aggttctggt acgattgatg acgacgacaa gagtccgggc
1981 ttctcctcaa cggatcccaa ttgggagctc gtgtacacgg cgcgcctgca ggtcgacggt
2041 accggttcga agcttgcggc cgcacagctg tatacacgtg caagccagcc agaactcgcc
2101 ccggaagacc ccgaggatct cgagcactaa gtgattaacc tcaggttata catatatttt
2161 gaatttaatt aattatacat atattttata ttatttttgt cttttattat cgaggggccg
2221 ttgttggtgt ggggttttgc atagaaataa caatgggagt tggcgacgtt gctgcgccaa
2281 caccacctcc cttccctcct ttcatcatgt atctgtagat aaaataaaat attaaaccta
2341 aaaacaagac cgcgcctatc aacaaaatga taggcattaa cttgccgctg acgctgtcac
2401 taacgttgga cgatttgccg actaaacctt catcgcccag taaccaatct agacgtcagg
2461 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
2521 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
2581 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
2641 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
2701 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
2761 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
2821 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
2881 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
2941 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
3001 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
3061 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
3121 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
3181 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
3241 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
3301 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
3361 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
3421 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
3481 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
3541 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
3601 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
3661 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
3721 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
3781 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3841 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
3901 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
3961 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
4021 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4081 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4141 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4201 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4261 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
4321 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
4381 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
4441 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
4501 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
4561 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga
4621 attcccggg
//
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pIEX-3质粒 pIEX3载体应用举例
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