| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET32 Xa/LIC, pet 32 Xa/LIC |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5926 bp |
| 5' 测序引物: | T7或者Trx-F |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; Trx-F: 5' TTCCTCGACGCTAACCTG 3' |
| 载体标签: | thioredoxin (N端); His (中间和C端), N- Factor Xa |
| 载体抗性: | Ampicillin |
| 表达宿主菌: | Origami2(DE3) 、 Origami(DE3)、OrigamiB(DE3)、BL21-TrxB(DE3) |
| 备注: |
For directional cloning of PCR-amplified DNA; ligation independent cloning; Factor Xa cleavage site
|
| 产品目录号: | 69072-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌2: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-32 Xa/LIC载体 pET32 Xa/LIC质粒基本信息
pET-32 Xa/LIC质粒图谱 pET32 Xa/LIC载体图谱和pET-32 Xa/LIC载体序列 pET32 Xa/LIC质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| S15 | 319 | 275 | |
| lacI | 328 | 2279 | |
| 6xHIS | 370 | 353 | |
| T7_transl_en_RBS | 743 | 727 | |
| lacO | 788 | 761 | |
| T7_promoter | 806 | 788 | |
| tet (300 - 563) | 842 | 1105 | |
| pBRrevBam_primer | 913 | 894 | |
| tet (576 - 851) | 2338 | 2613 | |
| ROP | 3088 | 3279 | |
| pGEX_3_primer | 3295 | 3273 | |
| pBR322_origin | 4313 | 3694 | |
| Ampicillin | 5328 | 4468 | |
| AmpR_promoter | 5398 | 5370 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 718 | 137 | |
| ORF frame 2 | 353 | 1447 | |
| ORF frame 3 | 1320 | 2279 | |
| ORF frame 2 | 5328 | 4468 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 206 |
| NcoI | 211 |
| AgeI | 257 |
| KpnI | 264 |
| BglII | 267 |
| BstBI | 294 |
| XbaI | 755 |
| BclI | 1561 |
| ApaI | 1758 |
| HpaI | 2053 |
| PstI | 4786 |
pET-32 Xa/LIC质粒 pET32 Xa/LIC载体简介
pET-32 Xa/LIC载体是用来快速定向PCR高通量质粒构建的载体。本载体带有N-Trx标签和His标签，S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-32 Xa/LIC质粒序列 pET32 Xa/LIC载体序列
LOCUS pET-32 Xa/LIC 5926 bp DNA Circular SYN
DEFINITION pET-32 Xa/LIC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5926
/organism="pET-32 Xa/LIC"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(137..718)
/label="ORF frame 1"
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
misc_feature complement(275..319)
/label="S15"
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
misc_feature 328..2279
/label="lacI"
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
FLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTGGGSGIE
GRSNSPLAMAISDPNSSSVDKLAAALEHHHHHH*"
CDS 353..1447
/label="ORF frame 2"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(353..370)
/label="6xHIS"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(727..743)
/label="T7_transl_en_RBS"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(761..788)
/label="lacO"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
promoter complement(788..806)
/label="T7_promoter"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
gene 842..1105
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(894..913)
/label="pBRrevBam_primer"
/translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ
SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV
RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS
ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG
AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES
IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG
TTTGGQTVVADWRCHLQSGPARAVANCRGD*"
CDS 1320..2279
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 2338..2613
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature 3088..3279
/label="ROP"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature complement(3273..3295)
/label="pGEX_3_primer"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
rep_origin complement(3694..4313)
/label="pBR322_origin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene complement(4468..5328)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(4468..5328)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5370..5398)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct
241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt
301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat
361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca
421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca
481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta
841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa
1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc
2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg
2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg
3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg
3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca
//
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pET-32 Xa/LIC质粒 pET32 Xa/LIC载体应用举例
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