| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET29c, pet 29c |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5372 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-S, N-Thrombin, C-His |
| 载体抗性: | Kanamycin |
| 备注: | N term thrombin cleavage site; a,b,c vary by MCS; |
| 产品目录号: | 69873-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)、BL21(DE3)pLysS |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-29c(+)载体 pET29c质粒基本信息
pET-29c(+)质粒图谱 pET29c载体图谱和pET-29c(+)载体序列 pET29c质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| lacI | 219 | 1858 | |
| T7_transl_en_RBS | 322 | 306 | |
| lacO | 367 | 340 | |
| T7_promoter | 385 | 367 | |
| tet (300 - 563) | 421 | 684 | |
| pBRrevBam_primer | 492 | 473 | |
| tet (576 - 851) | 1917 | 2192 | |
| ROP | 2667 | 2858 | |
| pGEX_3_primer | 2874 | 2852 | |
| pBR322_origin | 3892 | 3273 | |
| KanR2 | 3998 | 4813 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 298 | 1026 | |
| ORF frame 2 | 899 | 1858 | |
| ORF frame 2 | 3998 | 4813 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 209 |
| NcoI | 213 |
| KpnI | 239 |
| BglII | 242 |
| BstBI | 269 |
| NdeI | 296 |
| XbaI | 334 |
| BclI | 1140 |
| ApaI | 1337 |
| HpaI | 1632 |
| FspI | 2208 |
| NruI | 4086 |
| SmaI | 4303 |
| XmaI | 4301 |
pET-29c(+)质粒 pET29c载体简介
pET-29a-c载体带有N-S标签和C-His标签。本载体与pet25b载体相比，只有抗性标记不同。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-29c(+)质粒序列 pET29c载体序列
LOCUS pET-29c (+) 5372 bp DNA Circular SYN
DEFINITION pET-29c (+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5372
/organism="pET-29 c (+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
misc_feature 219..1858
/label="lacI"
CDS 298..1026
/label="ORF frame 1"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(306..322)
/label="T7_transl_en_RBS"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(340..367)
/label="lacO"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
promoter complement(367..385)
/label="T7_promoter"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
gene 421..684
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
misc_feature complement(473..492)
/label="pBRrevBam_primer"
/translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
TVVADWRCHLQSGPARAVANCRGD*"
CDS 899..1858
/label="ORF frame 2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 1917..2192
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature 2667..2858
/label="ROP"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature complement(2852..2874)
/label="pGEX_3_primer"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
rep_origin complement(3273..3892)
/label="pBR322_origin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 3998..4813
/label="KanR2"
/gene="KanR2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS 3998..4813
/label="ORF frame 2"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccacagata tcccatggaa ccgcgtggca ccagggtacc
241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatatg
301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac
421 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
481 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
541 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
601 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
661 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac
721 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
781 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
841 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
901 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
961 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
1021 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
1081 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
1141 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
1201 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
1261 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
1321 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
1381 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
1441 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
1501 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
1561 gcgcgttggt gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta
1621 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
1681 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
1741 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
1801 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
1861 gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct
1921 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
1981 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
2041 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
2101 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
2161 tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc
2221 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
2281 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
2341 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
2401 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
2461 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
2521 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
2581 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
2641 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
2701 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
2761 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
2821 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
2881 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
2941 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
3001 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt
3061 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
3121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
3181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
3241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
3421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
3481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
3541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
3601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
3661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
3721 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
3781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
3901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa
3961 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
4021 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
4081 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
4141 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
4201 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
4261 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca
4321 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
4381 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
4441 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
4501 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
4561 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
4621 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
4681 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
4741 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
4801 tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata
4861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta
4921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
4981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
5041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
5101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
5161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
5221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
5281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
5341 atgcgccgct acagggcgcg tcccattcgc ca
//
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pET-29c(+)质粒 pET29c载体应用举例
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