| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET23c, pet 23c |
| 质粒类型: | 大肠杆菌表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3664bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7, C-His |
| 载体抗性: | Ampicillin |
| 克隆菌株: | DH5a |
| 表达菌株: | BL21系列 |
| 备注: |
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
|
| 产品目录号: | 69747-3 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-23c(+)载体 pET23c质粒基本信息
pET-23c(+)质粒图谱 pET23c载体图谱和pET-23c(+)载体序列 pET23c质粒序列多克隆位点信息
The maps for pET-23b(+), pET-23c(+) and pET-23d(+) are the same as pET-23a(+) (shown) with the following exceptions: pET-23b(+) is a 3665bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-23c(+) is a 3664bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-23d(+) is a 3663bp plasmid; the BamH I site is in the same reading frame as in pET-23c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-23c(+). As a result, Nco I cuts pET-23d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-23a(+). Nde I does not cut pET-23d(+). Note also that Sty I is not unique in pET-23d(+).
pET-23c(+)质粒 pET23c载体简介
pET-23a-d系列载体带有N-T7标签和C-His标签。pET-23a-d系列载体与pET-21a-d系列载体相比，启动子由pet21a系列载体的T7lac启动子，更改为pET-23a-d载体的T7启动子。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-23c(+)质粒序列 pET23c载体序列
LOCUS pET-23c(+) 3664 bp DNA circular 11-SEP-2008
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(301..317)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
rep_origin complement(1448..1448)
/vntifkey="33"
/label=ColE1\origin
CDS complement(2209..3066)
/vntifkey="4"
/label=bla(amp)\sequence
rep_origin complement(3198..3653)
/vntifkey="33"
/label=f1\origin
BASE COUNT 919 a 935 c 925 g 885 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
3661 gcca
//
pET-23c(+)载体图谱 pET23c质粒图谱pdf版和pET-23c(+)载体序列 pET23c质粒序列等相关资料下载
pET-23c(+)质粒 pET23c载体应用举例
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