| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET21d, pet 21d |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5440bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7, C-His |
| 载体抗性: | Ampicillin |
| 备注: |
Same as pET24a, b, c, d(+) but ampR; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
|
| 产品目录号: | 69743-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-21d(+)载体 pET21d质粒基本信息
pET-21d(+)质粒图谱 pET21d载体图谱和pET-21d(+)载体序列 pET21d质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_leader | 237 | 205 | |
| T7_transl_en_RBS | 261 | 245 | |
| lacO | 306 | 279 | |
| T7_promoter | 324 | 306 | |
| tet (300 - 563) | 356 | 619 | |
| pBRrevBam_primer | 427 | 408 | |
| lacI | 702 | 1793 | |
| tet (576 - 851) | 1852 | 2127 | |
| ROP | 2602 | 2793 | |
| pGEX_3_primer | 2809 | 2787 | |
| pBR322_origin | 3827 | 3208 | |
| Ampicillin | 4842 | 3982 | |
| AmpR_promoter | 4912 | 4884 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 461 | 961 | |
| ORF frame 3 | 834 | 1793 | |
| ORF frame 2 | 4842 | 3982 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| NheI | 229 |
| NcoI | 234 |
| XbaI | 273 |
| BglII | 339 |
| BclI | 1075 |
| ApaI | 1272 |
| EcoRV | 1511 |
| HpaI | 1567 |
| PstI | 4300 |
pET-21d(+)质粒 pET21d载体简介
pET-21a-d载体具有N端T7标签和C端His标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-21d(+)质粒序列 pET21d载体序列
LOCUS pET-21d (+) 5440 bp DNA Circular SYN
DEFINITION pET-21 d (+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5440
/organism="pET-21 d (+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
misc_feature complement(205..237)
/label="T7_leader"
misc_feature complement(245..261)
/label="T7_transl_en_RBS"
misc_feature complement(279..306)
/label="lacO"
promoter complement(306..324)
/label="T7_promoter"
gene 356..619
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(408..427)
/label="pBRrevBam_primer"
CDS 461..961
/label="ORF frame 2"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
misc_feature 702..1793
/label="lacI"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
CDS 834..1793
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene 1852..2127
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature 2602..2793
/label="ROP"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
misc_feature complement(2787..2809)
/label="pGEX_3_primer"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
rep_origin complement(3208..3827)
/label="pBR322_origin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
gene complement(3982..4842)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(3982..4842)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4884..4912)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
241 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
301 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg
361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
601 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
661 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
721 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
781 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
841 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
901 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
961 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
1021 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
1081 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
1141 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
1201 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
1261 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
1321 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
1381 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
1441 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
1501 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
1561 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
1621 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
1681 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
1741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
1801 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
1861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
1921 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
1981 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
2041 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
2101 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg
2161 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
2221 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
2281 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
2341 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
2401 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
2461 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
2521 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
2581 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
2641 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
2701 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
2761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
2821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
2881 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat
3001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4261 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt
4981 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
5041 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
5101 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
5161 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
5221 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
5281 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
5341 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
5401 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
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