| 出品公司: | Novagen |
|---|---|
| 载体名称: | pET-3c, pET3c |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7 |
| 载体大小: | 4638 bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物及序列: | T7 Terminal: GCTAGTTATTGCTCAGCGG |
| 载体标签: | N-T7 |
| 载体抗性: | 氨苄青霉素 |
| 备注: | -- |
| 产品目录号: | 69420-3 |
| 稳定性: | 瞬时表达 |
| 组成型： | 非组成型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-3c载体 pET3c质粒基本信息
pET-3c质粒图谱 pET3c载体图谱和pET-3c载体序列 pET3c质粒序列多克隆位点信息
pET-3c质粒 pET3c载体简介
pET-3c质粒序列 pET3c载体序列
LOCUS pET-3c 4638 bp ds-DNA circular SYN 23-OCT-2018
DEFINITION Bacterial expression vector with a BamHI cloning site. For
alternative reading frames, use pET-3a or pET-3b.
ACCESSION .
VERSION .
KEYWORDS pET-3c
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4638)
AUTHORS MilliporeSigma (Novagen)
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4638
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 404..451
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(517..549)
/codon_start=1
/product="epitope tag from a T7 major capsid protein"
/note="T7 tag"
/translation="MASMTGGQQMG"
RBS 558..563
/note="ribosome binding site"
promoter complement(611..629)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2190..2381
/codon_start=1
/gene="rop"
/product="Rop protein"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
rep_origin complement(2811..3399)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3570..4430)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4431..4535)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
4561 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
4621 ccctttcgtc ttcaagaa
//
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