| 出品公司: | Novagen |
|---|---|
| 载体名称: | pET-38b(+), pET38b载体，pET38b质粒，pET-38b载体，pET-38b质粒 |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5821 bp |
| 5' 测序引物及序列: | T7 |
| 3' 测序引物及序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | His,C-CBD |
| 载体抗性: | 卡那霉素 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-38b(+)载体 pET38b质粒基本信息
pET-38b(+)质粒图谱 pET38b载体图谱和pET-38b(+)载体序列 pET38b质粒序列多克隆位点信息
载体图谱.jpg)
-1载体图谱.png)
pET-38b(+)质粒 pET38b载体简介
pET-38b（+）向量（Cat. No. 70137-3）用于表达C-终端CBDCEX 融合蛋白。插入N端信号序列和C端之间的目标序列CBDcex•Tag和His•Tag的阅读框（如下所示）中阅读，以产生所需的多肽 引信。有多种克隆地点和策略可供选择。独特的地点显示在圆圈上 地图。注意，序列由pBR322约定编号，因此T7表达式区域 在圆形地图上是相反的。T7转录的编码链的克隆/表达区 rna聚合酶如下所示。f1基因的起源是定向的，因此辅助噬菌体的感染将 产生含有与编码链相对应的单链DNA的病毒离子。因此， 应使用T7终止引物（Cat. No. 69337-3）。
pET-38b(+)质粒序列 pET38b载体序列
LOCUS pET-38b 5821 bp ds-DNA circular SYN 08-MAY-2022
DEFINITION synthetic circular DNA.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5821)
AUTHORS caoheibi
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5821)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sunday, May 8, 2022 from SnapGene Viewer 5.1.4.1 to Vector
NTI(R) format
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT VNTDATE|984960000|
COMMENT VNTDBDATE|984960000|
COMMENT VNTNAME|pET-38b---|
FEATURES Location/Qualifiers
source 1..5821
/organism="synthetic DNA construct"
/mol_type="other DNA"
/label=source
terminator 26..73
/label=terminator
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(150..173)
/codon_start=1
/product="8xHis affinity tag"
/label=8xHis affinity tag
/note="8xHis"
CDS complement(528..545)
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin recognition and cleavage site
/note="thrombin site"
RBS 754..776
/label=ribosome binding site
/note="efficient ribosome binding site from bacteriophageT7
gene 10 (Olins and Rangwala, 1989)"
protein_bind 791..815
/label=lac repressor encoded by lacI binding site
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator
toinhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(816..834)
/label=promoter
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 1147..1224
/gene="lacI"
/label=lacI promoter
/note="lacI promoter"
CDS 1225..2307
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="lacI"
/note="The lac repressor binds to the lac operator
toinhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
CDS 3116..3307
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low
copynumber"
/label=rop
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 3409..3551
/label=bom
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3737..4325)
/direction=LEFT
/label=ori
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 4447..5262
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=aph(3')-Ia
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
rep_origin complement(5355..5810)
/direction=LEFT
/label=f1 ori
/note="f1 ori"
/note="f1 bacteriophage origin of replication;
arrowindicates direction of (+) strand synthesis"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgacg
181 ccgaccgtgc agggcgtgcc gttgagcgag aacgccgtcg gcgcggcgtt ggtgcccgtg
241 tgcgagccgt tgaagccgaa ctgcgcggtg ccgcccgccg ggatcgagcc gttccacggg
301 gcgttgcgga ccgtcacggc cgagccggac tgcgtgaccg tcgagctcca cgcctgggtg
361 acctgctggc cggacgggaa gctgaacgtg agcgtccagc cgtctaccgg agcggaggac
421 gtgttcttca cggtgacgtt cgcggtgaag ccggtgttcc actggttgac gccccacagc
481 acctggcacc cggccggacc actagtcggg gtcggcgtcg gaggaccgga accgcgtggc
541 accagaccct cgagtgcggc cgcaagcttg acgtcctgca ggtgtacaga attcggatcc
601 gatatcacca tggtgctagc tgcctgagct ggcagaacaa cagttgcacc tacaaccagg
661 gtagcagcag cagcagccag agtagtacgc agagcagtac gagcaccacg agccggatga
721 cctggagctg gagtagtacg aggcatatgt atatctcctt cttaaagtta aacaaaatta
781 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
841 cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
901 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
961 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
1021 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
1081 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
1141 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
1201 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
1261 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
1321 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
1381 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
1441 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
1501 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
1561 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
1621 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
1681 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
1741 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
1801 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
1861 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
1921 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
1981 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc ggtagtggga
2041 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
2101 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
2161 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
2221 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
2281 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca
2341 ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg
2401 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
2461 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
2521 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
2581 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
2641 ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
2701 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
2761 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
2821 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
2881 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
2941 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
3001 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
3061 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
3121 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
3181 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
3241 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
3301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
3361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
3421 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
3481 gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
3541 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
3661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
3721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
3781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
3841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
3901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
4021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
4081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
4141 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
4201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4381 gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
4441 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac
4501 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
4561 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
4621 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
4681 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
4741 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
4801 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
4861 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
4921 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
4981 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
5041 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
5101 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
5161 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
5221 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg
5281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
5341 aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
5401 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
5461 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
5521 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
5581 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
5641 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
5701 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
5761 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
5821 a
//
