psiCHECK-2-h1-cluster22

LOCUS      psiCHECK-2-h1-cluster22              6604 bp DNA     circular SYN 02-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2003
                     /label=h1-cluster22
     polyA_signal    2019..2067
                     /note="synthetic polyadenylation signal"
     promoter        2076..2827
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2857..2866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2863..4515
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3822..3830
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4559..4680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4813..4917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4918..5778
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5949..6537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 aaattgaatt ccaaatatac ttagagatga tcttgacttt ctctgtaact ctaccacaac
     1741 atgcaataca tttaaggcct acatgggaaa tacactaatt agttgcataa cctttatgca
     1801 gaaatgaatg agaacagaag atcaccatca aaggaataaa agtcaataac tccgaaaaag
     1861 aagtaatatg cagatgggac atcttcacta agaaagtatg agtttaatgt agatattgtg
     1921 ggaggtattc ccttgtcatc tttgaattgg ttctttttaa cctgctttgt gaaggaggta
     1981 actcattaag cacgtttctt tacgcggccg ctggccgcaa taaaatatct ttattttcat
     2041 tacatctgtg tgttggtttt ttgtgtgagg atctaaatga gtcttcggac ctcgcggggg
     2101 ccgcttaagc ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact
     2161 ctcattcgga ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg
     2221 cgatcctctt aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta
     2281 actggcgggc cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc
     2341 tcgcagaccc ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca
     2401 tggggaccgt atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg
     2461 agaccttctg cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa
     2521 acaccaggac ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga
     2581 gcttcaggga gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
     2641 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
     2701 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt
     2761 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
     2821 cgcttaaaag cttggcattc cggtactgtt ggtaaagcca ccatggccga tgctaagaac
     2881 attaagaagg gccctgctcc cttctaccct ctggaggatg gcaccgctgg cgagcagctg
     2941 cacaaggcca tgaagaggta tgccctggtg cctggcacca ttgccttcac cgatgcccac
     3001 attgaggtgg acatcaccta tgccgagtac ttcgagatgt ctgtgcgcct ggccgaggcc
     3061 atgaagaggt acggcctgaa caccaaccac cgcatcgtgg tgtgctctga gaactctctg
     3121 cagttcttca tgccagtgct gggcgccctg ttcatcggag tggccgtggc ccctgctaac
     3181 gacatttaca acgagcgcga gctgctgaac agcatgggca tttctcagcc taccgtggtg
     3241 ttcgtgtcta agaagggcct gcagaagatc ctgaacgtgc agaagaagct gcctatcatc
     3301 cagaagatca tcatcatgga ctctaagacc gactaccagg gcttccagag catgtacaca
     3361 ttcgtgacat ctcatctgcc tcctggcttc aacgagtacg acttcgtgcc agagtctttc
     3421 gacagggaca aaaccattgc cctgatcatg aacagctctg ggtctaccgg cctgcctaag
     3481 ggcgtggccc tgcctcatcg caccgcctgt gtgcgcttct ctcacgcccg cgaccctatt
     3541 ttcggcaacc agatcatccc cgacaccgct attctgagcg tggtgccatt ccaccacggc
     3601 ttcggcatgt tcaccaccct gggctacctg atttgcggct ttcgggtggt gctgatgtac
     3661 cgcttcgagg aggagctgtt cctgcgcagc ctgcaagact acaaaattca gtctgccctg
     3721 ctggtgccaa ccctgttcag cttcttcgct aagagcaccc tgatcgacaa gtacgacctg
     3781 tctaacctgc acgagattgc ctctggcggc gccccactgt ctaaggaggt gggcgaagcc
     3841 gtggccaagc gctttcatct gccaggcatc cgccagggct acggcctgac cgagacaacc
     3901 agcgccattc tgattacccc agagggcgac gacaagcctg gcgccgtggg caaggtggtg
     3961 ccattcttcg aggccaaggt ggtggacctg gacaccggca agaccctggg agtgaaccag
     4021 cgcggcgagc tgtgtgtgcg cggccctatg attatgtccg gctacgtgaa taaccctgag
     4081 gccacaaacg ccctgatcga caaggacggc tggctgcact ctggcgacat tgcctactgg
     4141 gacgaggacg agcacttctt catcgtggac cgcctgaagt ctctgatcaa gtacaagggc
     4201 taccaggtgg ccccagccga gctggagtct atcctgctgc agcaccctaa cattttcgac
     4261 gccggagtgg ccggcctgcc cgacgacgat gccggcgagc tgcctgccgc cgtcgtcgtg
     4321 ctggaacacg gcaagaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtg
     4381 acaaccgcca agaagctgcg cggcggagtg gtgttcgtgg acgaggtgcc caagggcctg
     4441 accggcaagc tggacgcccg caagatccgc gagatcctga tcaaggctaa gaaaggcggc
     4501 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     4561 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     4621 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4681 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4741 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccagg tggcactttt
     4801 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4861 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4921 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4981 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5041 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5101 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5161 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5221 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5281 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5341 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5401 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5461 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5521 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5581 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5641 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5701 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5761 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5821 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5881 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6061 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct
     6601 cgac
//