| 质粒名称: | pCRISPR-PE-bacteria (pSPIN, pSC101* backbone) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 172715 |
| 存储实验室: | Tilmann Weber |
|
载体骨架基本信息 |
|
| 载体名称: | pCas9-bacteria |
| 载体出品公司: | Clontech |
| 空载体大小: | 2601bp |
| 完整质粒大小: | 8838bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Chloramphenicol, 25 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9n-linker-M-MLV2 |
| 物种来源: | Synthetic |
| 插入大小: | 6237bp |
| 基因突变: | H840A of Cas9 |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: |
pCRISPR-PE-bacteria was a gift from Tilmann Weber (Addgene plasmid # 172715 ; http://n2t.net/addgene:172715 ; RRID:Addgene_172715)
|
| 参考文献部分: | A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing. Tong Y, Jorgensen TS, Whitford CM, Weber T, Lee SY. Nat Commun. 2021 Sep 1;12(1):5206. doi: 10.1038/s41467-021-25541-3. 10.1038/s41467-021-25541-3 PubMed 34471126 |
买家导航
pCRISPR-PE-bacteria载体质粒基本信息
pCRISPR-PE-bacteria质粒图谱载体图谱和pCRISPR-PE-bacteria载体序列质粒序列多克隆位点信息
pCRISPR-PE-bacteria质粒载体简介
pCRISPR-PE-bacteria质粒序列载体序列
LOCUS pCRISPR-PE-bacteria 8838 bp DNA circular SYN 25-AUG-2024
DEFINITION Expression of an E. coli codon optimized fusion protein of
Cas9n-linker-M-MLV2 for prime editing.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8838)
AUTHORS Tong Y, Jorgensen TS, Whitford CM, Weber T, Lee SY
TITLE A versatile genetic engineering toolkit for E. coli based on
CRISPR-prime editing.
JOURNAL Nat Commun. 2021 Sep 1;12(1):5206. doi: 10.1038/s41467-021-25541-3.
PUBMED 34471126
REFERENCE 2 (bases 1 to 8838)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2021-09-1"; volume: "12(1):5206. doi"; pages: "
10.1038/s41467-021-25541-3"
FEATURES Location/Qualifiers
source 1..8838
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 541..643
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 644..1303
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(710..729)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
terminator 1324..1418
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 1532..2077
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 1978..1997
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2194..2211
/label=L4440
/note="L4440 vector, forward primer"
terminator complement(2239..2266)
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator complement(2282..2353)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(4513..8616)
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(H840A)
/note="generates RNA-guided single strand nicks in DNA due
to the H840A mutation in the HNH catalytic domain"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
RBS 8623..8634
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
promoter 8652..8707
/gene="tetR"
/label=tetR/tetA promoters
/note="overlapping promoters for bacterial tetR and tetA"
protein_bind 8653..8671
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS join(8723..8838,1..511)
/codon_start=1
/gene="tetR"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(8799..8818)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
ORIGIN
1 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
61 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
121 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
181 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
241 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
301 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
361 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
421 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
481 aaaacaactt aaatgtgaaa gtgggtctta agacgtcgat atctggcgaa aatgagacgt
541 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
601 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
661 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
721 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg catttttaaa
781 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
841 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
901 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
961 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
1021 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
1081 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
1141 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
1201 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
1261 tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata tcgagctcgc
1321 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
1381 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagt aacaacttat
1441 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga
1501 aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct
1561 cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta
1621 ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt
1681 cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg
1741 gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg
1801 gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
1861 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
1921 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg
1981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
2041 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc
2101 acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc
2161 atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
2221 tatatcccta ggtataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt
2281 agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
2341 ttcgttttat ttgatgcctg gagatcctta ctcgagttat tacgggctgc tattttcaat
2401 cagcagggtg ctggtatccg gggtttcggt aatcgccgct ttacgcgccg cttgatccgc
2461 catgcgatta ccacgcgcct ccgcgctgtg acctttctgg tgacccgggc agtgaatgat
2521 gctcagacgt ttcggcagga acagcgcttt cagcagcgcc agaatctcgt ccttattttt
2581 gatttctttg ccttcgctgg tcagccaacc acgacgacgg tagatttcac catggatgtg
2641 cgcggtcgca aacgcgtaac ggctatcggt ataaacattc agctttttgc cctccgccat
2701 tttcagcgct tgggtcagcg caatcagctc cgcacgctgc gcgctggtac ccgccggcag
2761 cgctttcgcc cagataactt cggtctcggt ggtaaccgcc gcacccgcct tacgctggcc
2821 ttcttgcagc aggctgctgc cgtcggtata ccaggtgtga tccgcgtccg gcagcggctg
2881 gtcggtcaga tccggacggg taccgtgcgc ctccgccaga atgtccaggc agttatgctg
2941 cagaccttcc tccggcagcg gcagcagggt cgccgggttc agcgcaacca ccggaccgaa
3001 ctgaacacgg tcggtatcca gcagcagcgc ttgatagtgg gtcatacgcg cgttgctcag
3061 ccaacggtcc ggcggctgct taaccagcgc ttcaaccgca tgcggcgcca gaatcaccag
3121 cggttgaccc atggtcagct tgcccgcgtc tttggtcaga accgcgatcg ccgcaaccat
3181 acgcaggcac ggcggccagc ccgccgcaac cggatccagt ttcttgctca ggtacgcaac
3241 cggacggcgc cacggaccca gtttctgggt cagaacgcct ttcgcataac cctgcttttc
3301 atcaacaaac agctcgaacg gcttggtcag gtccggcaga cccagcgccg gcgcggtcag
3361 cagcgcctgt ttgatctctt ggtacgcttt ctgctggtcc ggaccccagt taaacagggt
3421 acccggtttg gtcagcgggt acagcggcgc cgccatttcc gcgaaacccg ggataaacag
3481 acggcaaaaa cccgctttac ccaggaactc acgcagttgg cgcggggtct tcggggtcgg
3541 ctgacccata acggtttcct tacgcgcctc ggtcagccag cgttgacctt ccttcagcag
3601 gtagcccaga tacttcactt gcttctggca aatttgcgcc tttttcgcgc tcgcacggta
3661 acccaggtta cccagggtct gcagcagcgc acgggtaccc tgttggcaat ccagttcgct
3721 ggtcgccgcc agcagcagat cgtccacgta ctgcagcagg atcagatccg ggtgttggat
3781 acggaagtcc gccagatcac ggtgcagcgc ctcgttgaac agggtcgggc tgtttttaaa
3841 gccctgcggc agacgggtcc aggtcagttg gccgctgata cccatttccg ggtcacgcca
3901 ttcaaacgcg aacagcggtt ggctggtcgg gtgcagacgc aggcagaaaa acgcgtcctt
3961 cagatccagc acggtatacc actggtggct cggcggcaga ccgctcagca ggttgtacgg
4021 gttcggaacg gtcggatgaa tatcttcaac gcgcttgtta acctcacgca ggtcttgcac
4081 cggacggtaa tcattggtac ccggtttctt caccggcagc agcggggtat tccacgggct
4141 ctggcacggc accagaatac cctggtccag cagacgttga atgtgcggct taatacccag
4201 acgcgcttcc tggctcatcg gatactgctt gatgctaacc ggggtgctgg tcgccttcag
4261 cggaatgatc agcggcgcct ggcgaaccgc cagacccatg ccgccggttt ccgcccacgc
4321 ctgcggaaag tcgctcagcc aggtgctacc caggctaaca tccggctcct tgctggtctc
4381 atgcagacga tactcgtcct cgatgttcag ggtgctgcta ccgccgctgc tgccgccgct
4441 gctttccggg gtcgcgcttt cgctggtgcc cggggtttcg ctgccgctgc tgccgccgct
4501 gctgccgccg ctgtcacctc ctagctgact caaatcaatg cgtgtttcat aaagaccagt
4561 gatggattga tggataagag tggcatctaa aacttctttt gtagacgtat atcgtttacg
4621 atcaattgtt gtatcaaaat atttaaaagc agcgggagct ccaagattcg tcaacgtaaa
4681 taaatgaata atattttctg cttgttcacg tattggtttg tctctatgtt tgttatatgc
4741 actaagaact ttatctaaat tggcatctgc taaaataaca cgcttagaaa attcactgat
4801 ttgctcaata atctcatcta aataatgctt atgctgctcc acaaacaatt gtttttgttc
4861 gttatcttct ggactaccct tcaacttttc ataatgacta gctaaatata aaaaattcac
4921 atatttgctt ggcagagcca gctcatttcc tttttgtaat tctccggcac tagccagcat
4981 ccgtttacga ccgttttcta actcaaaaag actatattta ggtagtttaa tgattaagtc
5041 ttttttaact tccttatatc ctttagcttc taaaaagtca atcggatttt tttcaaagga
5101 acttctttcc ataattgtga tccctagtaa ctctttaacg gattttaact tcttcgattt
5161 ccctttttcc accttagcaa ccactaggac tgaataagct accgttggac tatcaaaacc
5221 accatatttt tttggatccc agtctttttt acgagcaata agcttgtccg aatttctttt
5281 tggtaaaatt gactccttgg agaatccgcc tgtctgtact tctgttttct tgacaatatt
5341 gacttggggc atggacaata ctttgcgcac tgtggcaaaa tctcgccctt tatcccagac
5401 aatttctcca gtttccccat tagtttcgat tagagggcgt ttgcgaatct ctccatttgc
5461 aagtgtaatt tctgttttga agaagttcat gatattagag taaaagaaat attttgcggt
5521 tgctttgcct atttcttgct cagacttagc aatcatttta cgaacatcat aaactttata
5581 atcaccatag acaaactccg attcaagttt tggatatttc ttaatcaaag cagttccaac
5641 gacggcattt agatacgcat catgggcatg atggtaattg ttaatctcac gtactttata
5701 gaattggaaa tcttttcgga agtcagaaac taatttagat tttaaggtaa tcactttaac
5761 ctctcgaata agtttatcat tttcatcgta tttagtattc atgcgactat ccaaaatttg
5821 tgccacatgc ttagtgattt ggcgagtttc aaccaattgg cgtttgataa aaccagcttt
5881 atcaagttca ctcaaacctc cacgttcagc tttcgttaaa ttatcaaact tacgttgagt
5941 gattaacttg gcgtttagaa gttgtctcca atagtttttc atctttttga ctacttcttc
6001 acttggaacg ttatccgatt taccacgatt tttatcagaa cgcgttaaga ccttattgtc
6061 tattgaatcg tctttaagga aactttgtgg aacaatggca tcgacatcat aatcacttaa
6121 acgattaata tctaattctt ggtccacata catgtctctt ccattttgga gataatagag
6181 atagagcttt tcattttgca attgagtatt ttcaacagga tgctctttaa gaatctgact
6241 tcctaattct ttgatacctt cttcgattcg tttcatacgc tctcgcgaat ttttctggcc
6301 cttttgagtt gtctgatttt cacgtgccat ttcaataacg atattttctg gcttatgccg
6361 ccccattact ttgaccaatt catcaacaac ttttacagtc tgtaaaatac cttttttaat
6421 agcagggcta ccagctaaat ttgcaatatg ttcatgtaaa ctatcgcctt gtccagacac
6481 ttgtgctttt tgaatgtctt ctttaaatgt caaactatca tcatggatca gctgcataaa
6541 attgcgattg gcaaaaccat ctgatttcaa aaaatctaat attgttttgc cagattgctt
6601 atccctaata ccattaatca attttcgaga caaacgtccc caaccagtat aacggcgacg
6661 tttaagctgt ttcatcacct tatcatcaaa gaggtgagca tatgttttaa gtctttcctc
6721 aatcatctcc ctatcttcaa ataaggtcaa tgttaaaaca atatcctcta agatatcttc
6781 attttcttca ttatccaaaa aatctttatc tttaataatt tttagcaaat catggtaggt
6841 acctaatgaa gcattaaatc tatcttcaac tcctgaaatt tcaacactat caaaacattc
6901 tatttttttg aaataatctt cttttaattg cttaacggtt acttttcgat ttgttttgaa
6961 gagtaaatca acaatggctt tcttctgttc acctgaaaga aatgctggtt ttcgcattcc
7021 ttcagtaaca tatttgacct ttgtcaattc gttataaacc gtaaaatact cataaagcaa
7081 actatgtttt ggtagtactt tttcatttgg aagattttta tcaaagtttg tcatgcgttc
7141 aataaatgat tgagctgaag cacctttatc gacaacttct tcaaaattcc atggggtaat
7201 tgtttcttca gacttccgag tcatccatgc aaaacgacta ttgccacgcg ccaatggacc
7261 aacataataa ggaattcgaa aagtcaagat tttttcaatc ttctcacgat tgtcttttaa
7321 aaatggataa aagtcttctt gtcttctcaa aatagcatgc agctcaccca agtgaatttg
7381 atggggaata gagccgttgt caaaggtccg ttgcttgcgc agcaaatctt cacgatttag
7441 tttcaccaat aattcctcag taccatccat tttttctaaa attggtttga taaatttata
7501 aaattcttct tggctagctc ccccatcaat ataacctgca tatccgtttt ttgattgatc
7561 aaaaaagatt tctttatact tttctggaag ttgttgtcga actaaagctt ttaaaagagt
7621 caagtcttga tgatgttcat cgtagcgttt aatcattgaa gctgataggg gagccttagt
7681 tatttcagta tttactctta ggatatctga aagtaaaata gcatctgata aattcttagc
7741 tgccaaaaac aaatcagcat attgatctcc aatttgcgcc aataaattat ctaaatcatc
7801 atcgtaagta tcttttgaaa gctgtaattt agcatcttct gccaaatcaa aatttgattt
7861 aaaattaggg gtcaaaccca atgacaaagc aatgagattc ccaaataagc catttttctt
7921 ctcaccgggg agctgagcaa tgagattttc taatcgtctt gatttactca atcgtgcaga
7981 aagaatcgct ttagcatcta ctccacttgc gttaataggg ttttcttcaa ataattgatt
8041 gtaggtttgt accaactgga taaatagttt gtccacatca ctattatcag gatttaaatc
8101 tccctcaatc aaaaaatgac cacgaaactt aatcatatgc gctaaggcca aatagattaa
8161 gcgcaaatcc gctttatcag tagaatctac caattttttt cgcagatgat agatagttgg
8221 atatttctca tgataagcaa cttcatctac tatatttcca aaaataggat gacgttcatg
8281 cttcttgtct tcttccacca aaaaagactc ttcaagtcga tgaaagaaac tatcatctac
8341 tttcgccatc tcatttgaaa aaatctcctg tagataacaa atacgattct tccgacgtgt
8401 ataccttcta cgagctgtcc gtttgagacg agtcgcttcc gctgtctctc cactgtcaaa
8461 taaaagagcc cctataagat tttttttgat actgtggcgg tctgtatttc ccagaacctt
8521 gaacttttta gacggaacct tatattcatc agtgatcacc gcccatccga cgctatttgt
8581 gccgatatct aagcctattg agtatttctt atccatagat cctttctcct ctttagatct
8641 tttgaattct tttctctatc actgataggg agtggtaaaa taactctatc aacgatagag
8701 tgtcaacaaa aattaggaat taatgatgtc tagattagat aaaagtaaag tgattaacag
8761 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
8821 gaagctaggt gtagagca
//
