pAAVLINKv2-SCP1-Cre-UDeg3a-FLNC-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-FLNC-C-FLAG                6800 bp DNA     circular SYN 22-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3513
                     /codon_start=1
                     /label=FLNC
                     /translation="VMGAWG*VLKAQARWTSTVRTWRTGHAKSPTAPPSPAPTSSTSSL
                     LTSTCLEAPSL*R*PARAA*RRASPGGDRHLPSPPSAAPVTSTSRSQETGSRWCLPRSA
                     *HAPSHAAATPTPARSARRSARRGAGRQSARCGWRSPPRSAGTPSLLCLGTSWAGSAWD
                     PSAASPGSRRVRPALRT*LHR*PAHRARWKPQRSSRARTAPTACALCPRKWGPIRSLSS
                     TVASTCPAAPFSSLWGRWVKVVPTRCGPEAQGWSEVWPACQPSSASGPGRLALGACPLL
                     WRVLAKRRLHLRIAKMAPAASPMSSRNQVTMRSPSSSMMSTSQTAPLWCLWPPSRMTLA
                     VSLSPASRRRGSR*TSQRPLPCS*TVPGA*LMPGCTHPRGLWRSATSLSWTVTSTPSAS
                     SPTRMASTPSMSSSTVPTSLEVPSRSALGSRARLGTQAWCQPTVLGSREALPVCHQSSS
                     *TP*MPARGPCLSPLMAPPRCSWTVGSVLRAMWSLILPWPLATTSLPSSTVAPSTSWAA
                     PSRPRSLVRGCPEATAFTKHPRFWWRL*PSPPQAGAPATAPSPSSPQMPARW*LGALGC
                     PRPSWARRTPSPWTAAKQAPT**WWACTAPRPPVRRCT*STWGTGCTMSPTLSRRKGTT
                     SSLSSGVTKVSLEAPSKSRS"
     misc_feature    2060..2065
                     /label=SDS
     misc_feature    3188..3193
                     /label=SDS
     misc_feature    3377..3382
                     /label=SDS
     CDS             3520..3585
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3589..4020
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3733..3738
                     /label=SDS
     repeat_region   4063..4203
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4063..4192
                     /label=AAV2 ITR
     rep_origin      4278..4733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5015..5119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5120..5980
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6151..6739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtta tgggggcttg
     1561 gggctgagta ttgaaggccc aagcaaggtg gacatcaact gtgaggacat ggaggacggg
     1621 acatgcaaag tcacctactg ccccaccgag cccggcacct acatcatcaa catcaagttt
     1681 gctgacaagc acgtgcctgg aagccccttc actgtgaagg tgaccggcga gggccgcatg
     1741 aaggagagca tcacccggcg gagacaggca ccttccatcg ccaccatcgg cagcacctgt
     1801 gacctcaacc tcaagatccc aggaaactgg ttccagatgg tgtctgccca ggagcgcctg
     1861 acacgcacct tcacacgcag cagccacacc tacacccgca cggagcgcac ggagatcagc
     1921 aagacgcggg gcggggagac aaagcgcgag gtgcgggtgg aggagtccac ccaggtcggc
     1981 ggggacccct tccctgctgt gtttggggac ttcctgggcc gggagcgcct gggatccttc
     2041 ggcagcatca cccggcagca ggagggtgag gccagctctc aggacatgac tgcacaggtg
     2101 accagcccat cgggcaaggt ggaagccgca gagatcgtcg agggcgagga cagcgcctac
     2161 agcgtgcgct ttgtgcccca ggaaatgggg ccccatacgg tcgctgtcaa gtaccgtggc
     2221 cagcacgtgc ccggcagccc ctttcagttc actgtggggc cgctgggtga aggtggtgcc
     2281 cacaaggtgc gggccggagg cacagggctg gagcgaggtg tggccggcgt gccagccgag
     2341 ttcagcatct ggacccggga ggctggcgct gggggcctgt ccattgctgt ggagggtcct
     2401 agcaaagcgg agattgcatt tgaggatcgc aaagatggct cctgcggcgt ctcctatgtc
     2461 gtccaggaac caggtgacta tgaggtctcc atcaagttca atgatgagca catcccagac
     2521 agcccctttg tggtgcctgt ggcctccctc tcggatgacg ctcgccgtct cactgtcacc
     2581 agcctccagg agacggggct caaggtgaac cagccagcgt cctttgccgt gcagctgaac
     2641 ggtgcccggg gcgtgattga tgcccgggtg cacacaccct cgggggctgt ggaggagtgc
     2701 tacgtctctg agctggacag tgacaagcac accatccgct tcatccccca cgagaatggc
     2761 gtccactcca tcgatgtcaa gttcaacggt gcccacatcc ctggaagtcc cttcaagatc
     2821 cgcgttgggg agcagagcca ggctggggac ccaggcttgg tgtcagccta cggtcctggg
     2881 ctcgagggag gcactaccgg tgtgtcatca gagttcatcg tgaacaccct gaatgccggc
     2941 tcgggggcct tgtctgtcac cattgatggc ccctccaagg tgcagctgga ctgtcgggag
     3001 tgtcctgagg gccatgtggt cacttatact cccatggccc ctggcaacta cctcattgcc
     3061 atcaagtacg gtggccccca gcacatcgtg ggcagcccct tcaaggccaa ggtcactggt
     3121 ccgaggctgt ccggaggcca cagccttcac gaaacatcca cggttctggt ggagactgtg
     3181 accaagtcct cctcaagccg gggctccagc tacagctcca tccccaagtt ctcctcagat
     3241 gccagcaagg tggtgactcg gggccctggg ctgtcccagg ccttcgtggg ccagaagaac
     3301 tccttcaccg tggactgcag caaagcaggc accaacatga tgatggtggg cgtgcacggc
     3361 cccaagaccc cctgtgagga ggtgtacgtg aagcacatgg ggaaccgggt gtacaatgtc
     3421 acctacactg tcaaggagaa aggggactac atcctcattg tcaagtgggg tgacgaaagt
     3481 gtccctggaa gccccttcaa agtcaaggtc ccttgtacag actacaaaga ccatgacggt
     3541 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact cgagataatc
     3601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3721 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
     3781 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3841 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt
     3901 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     3961 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
     4021 agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     4081 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4141 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4201 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4261 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4321 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4381 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4441 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4501 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4561 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     4621 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     4681 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4741 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4801 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4861 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4921 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4981 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6541 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6781 ctggcctttt gctcacatgt
//