| 质粒名称: | pAAV-CAG-tdTomato-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000717 |
| 产品用途: | AAV, experess tdTomato and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | tdTomato, EGFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-tdTomato-P2A-EGFP载体质粒基本信息
pAAV-CAG-tdTomato-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-tdTomato-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-tdTomato-P2A-EGFP质粒载体简介
pAAV-CAG-tdTomato-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-tdTomato-P2A-EGFP 6961 bp DNA circular SYN 28-DEC-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6961)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6961
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 919..941
/label=Seq-F
primer_bind complement(934..982)
/label=Vectore-R
primer_bind 959..1004
/label=tdTomato-F
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 983..2410
/label=tdTomato
primer_bind complement(2388..2432)
/label=tdTomato-R
primer_bind 2411..2458
/label=Vector-F
CDS 2435..2491
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2504..3220
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 3245..3833
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3716..3727)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3876..3997
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4525..5113)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5284..6144)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6145..6249)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6275..6730)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgag taagggcgag gaagtgatca aagagttcat
1021 gcggtttaag gtgagaatgg aaggaagcat gaacggccac gagttcgaaa ttgagggaga
1081 aggagaggga cggccctacg agggcaccca gacagccaag ctgaaagtga caaagggcgg
1141 gcctctgcca ttcgcttggg acatcctgag cccacagttt atgtacggct ccaaggccta
1201 tgtgaaacat ccagctgaca ttcccgatta taagaaactg agcttccccg aggggtttaa
1261 gtgggaaaga gtgatgaact tcgaggacgg aggcctggtg actgtgaccc aggacagctc
1321 cctgcaggat gggaccctga tctacaaggt gaaaatgaga gggacaaatt ttccccctga
1381 tggacctgtg atgcagaaga aaactatggg atgggaggcc tccaccgaaa ggctgtatcc
1441 acgcgacggg gtgctgaaag gagaaatcca ccaggctctg aagctgaaag atgggggaca
1501 ttacctggtg gagttcaaga caatctacat ggccaagaaa cctgtgcagc tgccaggcta
1561 ctattacgtg gacacaaaac tggatatcac ttcacacaac gaggactaca ctattgtgga
1621 gcagtatgaa cggagcgagg ggagacacca tctgttcctg ggccatggga ctggaagtac
1681 cggctcaggg tctagtggaa ccgcctcaag cgaggataac aatatggctg tgatcaaaga
1741 gttcatgagg tttaaggtgc gcatggaggg cagcatgaat gggcacgaat ttgagattga
1801 aggagagggc gaagggaggc cttacgaggg cacacagact gccaagctga aagtgaccaa
1861 gggaggacca ctgcctttcg cttgggatat cctgtctcct cagtttatgt acggaagtaa
1921 ggcctatgtc aagcatcccg ctgacattcc tgattacaag aaactgtctt tcccagaggg
1981 ctttaagtgg gagagagtga tgaattttga agatggaggc ctggtgaccg tgacacagga
2041 ctcctctctg caggatggca ctctgatcta caaagtcaaa atgcgcggca ccaattttcc
2101 acccgatggg cccgtgatgc agaagaaaac aatggggtgg gaggccagca ctgaacggct
2161 gtatcctaga gacggagtgc tgaagggcga aatccaccag gccctgaagc tgaaagacgg
2221 cggccactac ctggtggagt tcaaaaccat ctacatggcc aagaaaccag tgcagctgcc
2281 cggctattac tatgtggaca ccaagctgga tatcacatcc cacaatgaag actacaccat
2341 tgtggaacag tatgagaggt ctgaaggacg ccaccatctg tttctgtacg gcatggatga
2401 gctgtataag cgaccggttc acgtgggaag tggggctaca aacttctcac tgctgaaaca
2461 ggccggggat gtggaggaaa acccaggacc acacgtgact agtgtgagca agggcgagga
2521 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
2581 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
2641 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
2701 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
2761 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
2821 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
2881 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
2941 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
3001 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
3061 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
3121 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
3181 cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcgata tcaagcttat
3241 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
3301 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
3361 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
3421 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
3481 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
3541 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
3601 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
3661 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
3721 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
3781 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
3841 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
3901 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
3961 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
4021 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
4081 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
4141 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
4201 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
4261 cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag
4321 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4381 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4441 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4501 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4561 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
4621 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
4681 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
4741 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
4801 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
4861 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
4921 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
4981 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5041 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5101 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5161 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5221 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5281 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5341 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5401 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5461 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5521 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
5581 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
5641 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
5701 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
5761 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
5821 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
5881 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
5941 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
6001 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
6061 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
6121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
6181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
6241 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
6301 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
6361 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
6421 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
6481 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
6541 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
6601 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
6661 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
6721 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
6781 gcctcttcgc tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
6841 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
6901 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
6961 t
//
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