| 质粒名称: | pCytERM-(n1)StayGold(E138D)(c4)-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000290 |
| 产品用途: | Expresses CytERM-(n1)StayGold(E138D)(c4) in mammalian cells culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | CytERM-(n1)StayGold(E138D)(c4) |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
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pCytERM-(n1)StayGold(E138D)(c4)-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCytERM-(n1)StayGold(E138D)(c4)-N1载体质粒基本信息
pCytERM-(n1)StayGold(E138D)(c4)-N1质粒图谱载体图谱和pCytERM-(n1)StayGold(E138D)(c4)-N1载体序列质粒序列多克隆位点信息
StayGold(E138D)(c4)-N1载体图谱.png)
pCytERM-(n1)StayGold(E138D)(c4)-N1质粒载体简介
pCytERM-(n1)StayGold(E138D)(c4)-N1质粒序列载体序列
LOCUS pCytERM-(n1)StayGold(E138D)(c4)-N1 4797 bp DNA circular SYN 01-NOV-2023
DEFINITION Vector for fusing EGFP to the C-terminus of a partner protein. For
other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION U55762
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4797)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Jul 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4797
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 615..701
/codon_start=1
/product="cytochrome P-450 IIC1, partial [Oryctolagus
cuniculus]
Sequence ID: AAA31436.1Length: 56Number of Matches: 1"
/label=cytochrome P-450
/translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
primer_bind 720..774
/label=0830-CYT-N1C4-STAY-F
CDS 753..1460
/codon_start=1
/label=(n1)mStayGold(E138D)(c4）
/translation="MASTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNDVQHIPRDDGVECPVTL
LYPLLSDKSKCVEAHQNTICKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHICQSETL
EAHLPWHEPSASAV"
misc_feature 765..791
/label=n1
primer_bind 1172..1227
/label=0707-2-E138D-F
misc_feature 1191..1193
/label=E138D
misc_feature 1431..1460
/label=c4
primer_bind complement(1440..1491)
/label=0830-CYT-N1C4-STAY-R
polyA_signal 1585..1706
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1713..2168)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2195..2299
/gene="bla"
/label=AmpR promoter
promoter 2301..2658
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2509..2644
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2693..3487
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3719..3766
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4095..4683
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctatggac cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc
661 tcctttcact ctggaaacag agctatgggg gagggaagct tcgaattctg cagtcgacgg
721 taccgcgggc ccgggatcca ccggtcgcca ccatggctag tacaggggag gagctgttca
781 ccggggtggt gccatttaaa tttcaactta aaggaaccat caatggcaaa tcgtttaccg
841 ttgaaggcga aggtgaagga aattcacatg aaggttctca taaaggaaaa tatgtttgta
901 caagtggaaa actaccgatg tcatgggcag cacttggaac atcctttggt tatggaatga
961 aatattatac caaatatcct agtggactga agaactggtt tcatgaagta atgcctgaag
1021 gctttaccta cgatcgtcat attcaatata aaggcgatgg gagtatccat gcaaaacacc
1081 aacactttat gaaaaatggg acttatcaca acattgttga atttactggt caggatttta
1141 aagaaaatag tccagtctta actggagata tgaatgtctc attaccgaat gacgttcaac
1201 atatacccag agatgatgga gtagaatgcc cagtgacctt gctttatcct ttattatcgg
1261 ataaatcaaa atgcgttgag gctcaccaaa atacaatctg caagcctctt cataatcaac
1321 cagcacctga tgtcccatat cactggattc gtaaacaata cacacaaagc aaagatgata
1381 ccgaggaacg tgatcatatt tgtcaatcag agactctcga agcacactta ccatggcacg
1441 agccttctgc ttctgccgtg taagcggccg cgactctaga tcataatcag ccataccaca
1501 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
1561 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
1621 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
1681 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
1741 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
1801 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
1861 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
1921 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
1981 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
2041 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
2101 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
2161 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
2221 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
2281 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
2341 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2401 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2461 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2521 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2581 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2641 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
2701 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
2761 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
2821 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
2881 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
2941 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
3001 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
3061 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
3121 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
3181 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
3241 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
3301 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
3361 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
3421 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
3481 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
3541 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
3601 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
3661 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
3721 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
3781 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
3841 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
3901 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
3961 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
4021 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4081 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4141 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4201 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
4261 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
4321 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
4381 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
4441 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
4501 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
4561 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
4621 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
4681 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
4741 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
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