| 质粒名称: | pAAV-hSyn-RGEPO2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000766 |
| 产品用途: | Expressing the red potassium indicator in the cytoplasm of neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-Syn |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | RGEPO2 |
| 物种来源: |
bacterium
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.
Yang L., Pathiranage V., Zhou S., Sun X., Zhang H., Lai C., Gu C., Subach F.V., Drobizhev M., Walker A.R., Piatkevich K.D.
PLOS Biol. 23, e3002993 (2025)
|
pAAV-hSyn-RGEPO2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-hSyn-RGEPO2载体质粒基本信息
pAAV-hSyn-RGEPO2质粒图谱载体图谱和pAAV-hSyn-RGEPO2载体序列质粒序列多克隆位点信息
pAAV-hSyn-RGEPO2质粒载体简介
pAAV-hSyn-RGEPO2质粒序列载体序列
LOCUS pAAV-hSyn-RGEPO2 5602 bp DNA circular SYN 15-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5602)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5602
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 311..800
/label=Syn
promoter 349..796
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
primer_bind complement(548..557)
/label=PA-S9A-F1
primer_bind complement(700..710)
/label=0821_FRCaMPi_R
primer_bind complement(701..710)
/label=PD-R1
primer_bind 734..755
/label=Syn-F
primer_bind 787..830
/label=Intra-F1
primer_bind 787..821
/label=Asp-syn-F1
/note="C08"
primer_bind 787..820
/label=Lucy-syn-F1
primer_bind 787..820
/label=Azu-syn-F1
primer_bind 811..835
/label=N-mapple-F2
misc_feature 812..817
/label=Kozak sequence
CDS 818..1270
/codon_start=1
/label=N-mApple
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
primer_bind 1244..1291
/label=FR-1-3-F
primer_bind 1244..1265
/label=FR-Y-Ra-F
primer_bind complement(1257..1270)
/label=N-mapple-R
primer_bind complement(1259..1277)
/label=FR-1-3-R
primer_bind complement(1266..1284)
/label=0905-R1
primer_bind 1267..1306
/label=PA-S9A-F1
primer_bind 1268..1300
/label=PA-F6A-F1
primer_bind 1268..1291
/label=PA-K8A-F1
CDS 1271..1273
/codon_start=1
/label=LINKER1
/translation="L"
CDS 1274..1705
/codon_start=1
/label=YgaU_Hydrothermal
/translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
primer_bind 1307..1324
/label=0811-F2
primer_bind 1464..1496
/label=PA-T73A-F1
primer_bind 1468..1502
/label=PA-G75A-F1
primer_bind complement(1468..1485)
/label=0905-R2
primer_bind 1505..1523
/label=0811-F1
CDS 1706..1714
/codon_start=1
/label=LINKER
/translation="SNP"
CDS 1715..1972
/codon_start=1
/label=C-mApple
/translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
primer_bind complement(1953..1972)
/label=0821_FRCaMPi_R
primer_bind complement(1954..1972)
/label=PD-R1
primer_bind complement(1956..1998)
/label=Intra-R1
primer_bind complement(1956..1975)
/label=c-mapple-PCI-R
primer_bind 1958..1972
/label=0821_KA_FRCaMPi_F
primer_bind complement(1973..1998)
/label=Asp-syn-R1
/note="C08"
misc_feature 2000..2588
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2471..2482)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2606..2813
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 2865..3005
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3080..3535
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3817..3921
/gene="bla"
/label=AmpR promoter
CDS 3922..4782
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4953..5541
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg gtgagcaagg gcgaggagga
841 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
901 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg ccttccagac
961 cgctaggctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
1021 tcagttcatg tacggctcca aggcctacat taagcaccca gccgacatcc ccgactactt
1081 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgagcttcg aggacggcgg
1141 cattattcac gttaatcagg actcctccct gcaggacggc gtattcatct acaaggtgaa
1201 gctgctcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
1261 ggaggcttcc ctgggcatct gggacttcgt gaagagcgcc ggcaggaaga tcggcatcgg
1321 cagcgaggag gccgcccccg ccgccgacga cctgaagaag gagctggaca gctacaagct
1381 gggcaccgac gacgtgaagg tggaggtggt gggcgacaag gccgtgatca ccggcaacgt
1441 ggccgaccag agcatcctgg agaaggccat cgtggccgtg ggcaacaccc tgggcatcag
1501 caaggtggag agcaacgtgg aggtgcccga cgccaaggag cccgtgttcc acaccgtgaa
1561 gaagggcgac accctgtggg ccatcgccag caagcagtac ggcaacgtca gcaagtacaa
1621 cgccatcttc gaggccaaca agcccatgct gagccacccc gacaagatct accccggcca
1681 ggtgctgagg atccccgacc tgggctctaa cccggtttcc gagtggatgt accccgagga
1741 cggcgccctg aagagcgaga tcaggaaggg gctgaggctg aaggacggcg gccactacgc
1801 cgccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat
1861 cgtcgacatc gagttggaca tcttgtccca caacgaggac tacaccatcg tggaacagtg
1921 cgaacgcgcc gtgggccgcc accccaccgg tggcacggac gcgctgtaca agtaggaatt
1981 ccgtacaaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
2041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
2101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
2161 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
2221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
2281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
2341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
2401 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
2461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
2521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
2581 ctccccgcat cgataccggc tcgagctgtg ccttctagtt gccagccatc tgttgtttgc
2641 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
2701 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
2761 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtc
2821 tcgagctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
2881 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
2941 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
3001 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
3061 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
3121 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
3181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
3241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
3301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
3361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
3421 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
3481 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
3541 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
3601 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
3661 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
3721 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
3781 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
3841 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
3901 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
3961 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
4021 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
4081 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
4141 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
4201 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
4261 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
4321 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
4381 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
4441 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
4501 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
4561 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
4621 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
4681 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
4741 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
4801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
4861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
4921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
4981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
5041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
5101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
5161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
5221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
5281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
5341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
5401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
5461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
5521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
5581 tgctggcctt ttgctcacat gt
//
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