| 质粒名称: | pRS414-Syf1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 234114 |
| 存储实验室: | Aaron Hoskins |
|
载体骨架基本信息 |
|
| 载体名称: | pRS414 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 7878bp |
| 载体类型: | Yeast Expression |
| 筛选标记: | TRP1 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Syf1 |
| 物种来源: | S. cerevisiae (budding yeast) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Unknown (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pRS414-Syf1 was a gift from Aaron Hoskins (Addgene plasmid # 234114 ; http://n2t.net/addgene:234114 ; RRID:Addgene_234114) |
买家导航
pRS414-Syf1载体质粒基本信息
pRS414-Syf1质粒图谱载体图谱和pRS414-Syf1载体序列质粒序列多克隆位点信息
pRS414-Syf1质粒载体简介
pRS414-Syf1质粒序列载体序列
LOCUS pRS414-Syf1 7878 bp DNA circular SYN 15-DEC-2025
DEFINITION Syf1 region PCR, restriction digest and ligation into pRS414
backbone.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7878)
TITLE Hoskins lab plasmids 2025
REFERENCE 2 (bases 1 to 7878)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: pRS414-Syf1
FEATURES Location/Qualifiers
source 1..7878
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 13..34
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(274..948)
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
promoter complement(949..1229)
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
primer_bind complement(1250..1269)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1369..1391
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1429..1447)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
misc_feature 1488..1991
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
promoter 2028..2132
/gene="bla"
/label=AmpR promoter
CDS join(2133..2201,2202..2993)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2351..2370)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3164..3752
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3653..3672
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3906..3923
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 4040..4061
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(4076..4081,4082..4099,4100..4106)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4114..4130
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4119..4141
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4138..4154
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4138..4154
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 4173..4193
/label=T3
/note="T3 promoter, forward primer"
promoter 4175..4193
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7408..7427)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7409..7427)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7437..7454)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7437..7453)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7446..7468)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin join(7594..7878,1..171)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7681..7700)
/label=F1ori-R
/note="F1 origin, reverse primer"
ORIGIN
1 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
61 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
121 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
181 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatcggc aagtgcacaa
241 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
301 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
361 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
421 cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca
481 atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg
541 tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat
601 aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac
661 gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa
721 cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta caagacttga
781 aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc
841 cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct gcaagccgca
901 aactttcacc aatggaccag aactacctgt gaaattaata acagacatac tccaagctgc
961 ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc ctccctcttg
1021 gccctctcct tttctttttt cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc
1081 cggatcaaga ttgtacgtaa ggtgacaagc tatttttcaa taaagaatat cttccactac
1141 tgccatctgg cgtcataact gcaaagtaca catatattac gatgctgttc tattaaatgc
1201 ttcctatatt atatatatag taatgtcgtg atctatggtg cactctcagt acaatctgct
1261 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
1321 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
1381 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
1441 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacggat cgcttgcctg
1501 taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt
1561 tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt
1621 acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt
1681 acatatatat ttattagaca agaaaagcag attaaataga tatacattcg attaacgata
1741 agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca
1801 attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat
1861 ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt
1921 ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata attattttta
1981 tagcacgtga tgaaaaggac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
2041 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
2101 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
2161 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
2221 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
2281 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
2341 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
2401 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
2461 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
2521 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
2581 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
2641 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
2701 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
2761 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
2821 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
2881 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
2941 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
3001 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
3061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
3121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
3181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
3241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
3301 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
3361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
3421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
3481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
3541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
3601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
3661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
3721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
3781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
3841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
3901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
3961 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
4021 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
4081 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
4141 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
4201 agctggagct ccaccgcggt ggcggccgca gtcacgcaaa cggatctctg tttacattag
4261 cattgctatc taagaggata atctgggtta tgctttatgg tctatgaagt acctgtttta
4321 tgatattgtt tatttcactt ccttaaacaa aatatctgta tgttatgaaa gcaaaataca
4381 caacccaaac atccacagtg cacggatgtt tgaaggaaaa ttgctactta atcagtaatt
4441 gcagaaggcc gcaccaaaaa agaacttatg gtttcgaaaa tgatgcatga ttttacatag
4501 cttatatcaa atatctagtt ctatttcatc tggattaatc gaatatgaag aggttgattg
4561 ggtaagagta tgcgagttgg gagcatgcga ggttgctgcc gccacgaagt taatgttacc
4621 ttcctcatgg ctaacgctag ctgaatcaat gagcatgttg gattccaaca ccttcttcat
4681 cttcaacatg tctttgtaag tttccttatc cccatgtttt aactcaaaaa tttcaaagct
4741 atcccataat tcagtatttc tggatggagg aagaagcttt gcaccatagg caaggatttc
4801 acgtgctcga atagtttctc ctatagagct ctcgaaatct gaaaatttaa tcacaaactc
4861 aacagcttta gagtttggta aaatttgaat gcattcttgg tacaactctc tggttactga
4921 tggtcccaaa gtagactcgg ccttggaaat acacattctc cacagttgca gtctactttc
4981 gaggtgcgtg ctcacactaa cagtacctat tacagcacct ctacgtaata tctcaatact
5041 cttgctaatc agaccactta tcctttcttc aaatacgcta taagcgataa aaattgtttt
5101 acagtctata ccatttgaac atagattctt aagtgctttc tcaaacaaaa atctaatatg
5161 ttcaggactc agtgatgaca attgatgtga tgtcgctact tctagatact caatccataa
5221 ttcgtattgg atttcggggg gaaacagagg gattgttttc tcatacacct gaaaactttc
5281 catgacttcg taatgatttt gtaagaacaa tgcaaaattt tctgccattg ctggagtgat
5341 caatctcaaa tcaataacag tattgtatgc catttttgtc ttattgaata ttttatcgga
5401 ggaatttgca tcctttgggc agtaagcctc tagataatca atgtatttgg accaaatacg
5461 taatgagttg aatagaacag tttgagcagg tatttttctg tgtccattct tatatttttc
5521 gaggagaatt tctggatttg ttggcacatg taatgcatct tcaaggatag aaaaagctct
5581 ttccacccct tctttatcta attctctatc agcccagttt aggtaaattt cttccagatc
5641 ttctatataa ggatacggta ccttcagaga ttgtgtccac aattctctag ctgtactaat
5701 tgcgtttgat ctccaataca aatccccata cgaacaccaa agcctaccga aagagccagg
5761 cgtaccaact tttcggggat caattttcag tatagcttct gaatacacat tacacttttc
5821 cgctgctgac ttctgcaagg acactctttt catccacgtt tccaccaagt tggaatcttg
5881 ccttagtgcg acatcgttca aatagatatc atataagttg attaaagatt caaatgtcgc
5941 catatgagct tgtaactttt cagtccaatc tttttgatta aagaatttgc tgtcattttc
6001 ctttagcttt cccagaataa attgggagca ctcttgctca aatagcaaat agaaattata
6061 gatgcgatca aaatcactgt acctaagtgt ttgctgtaaa cttttcttca ataaatcacc
6121 acaactgtca agcctcccgc gagagatata atatttggca agtgatagga tcaaaaaaag
6181 ccatttgtcg ggatatatgc cattcatttg cctcatgaac tcctcatatt gattatctaa
6241 acctagtttt tctaggctgg ccaaataatt gaaatttaat tcaaaaggca gttctgaact
6301 aggtagatac ttcccactat tttcatcttg tggcaaatac ttttcatata ctgatttgat
6361 ggtaatattg tctctagtta atgcaagagt tgccagcgat tcatttcgtt tttgctgagg
6421 agccactttt aagtatcttt ctaaaatatg tgacgaccaa atatcacctc tacttccgtt
6481 ttcactaatt tcctcgctaa taaaacctcc cttcgtgaaa cccttcacta aaagtacatt
6541 tatcaattca gcttcatcag tactttcttc ttcatcttct tgagttgaat ccagttgcgt
6601 taatggtaaa actttttctt ccacaaattt tatgacagga tcccaaactt tccgatgcat
6661 ttctctctcc attttcatta aagatgaagc taaggcatgc cttatcattg ccaagtcata
6721 ttgttcaatt gccaattcta aataagacaa acatatcctg tcacaatctc gtacacagct
6781 tttcaggcac ctttgaaaaa gccagaaaat acgtgaagtt tcgactactt cctttgttga
6841 ttcccagcgt atataatctt cccatatact tgtatctgtt acaaattgtg aacaaaatct
6901 ttcatatagc cacctaatct gtttgtcagt tcttccctcc tctttccaat actcgatata
6961 tcttttccat gtaagtatgt tctgcggtgt cttttgtatt tcgtactcaa aagccacatc
7021 ttcatcattt cttatgttct catcgacgtt tgttattact cctttcattg cgatgtatgc
7081 tgacatagga tatatggaca caataagttt tgcggtactg aaggatagtt gagtccaata
7141 tggaggcctg attatttcct ttttttctat ctatttcctt ttgtattatt ttgactgaaa
7201 agcttgccag cgtctttact aaaatctttc atatgttcgg accgctatag taaactgaaa
7261 cttgatattc aataattatc aatcttcgaa gctaaagaaa tacaaaatct ttattcgaaa
7321 aagtaagtaa cataatcaac cgctgattag cacaatatga ggatacagtc gctgtcgacc
7381 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
7441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
7501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
7561 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
7621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
7681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
7741 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
7801 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
7861 ctttgacgtt ggagtcca
//
