| 质粒名称: | pGL3-BAG3-Nicking/PegRNA-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002174 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting BAG3 gene NickingRNA and pegRNA, harboring selection markers of puroR. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BAG3-Nicking-PegRNA |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-BAG3-Nicking/PegRNA-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-BAG3-Nicking/PegRNA-PGK-puroR载体质粒基本信息
pGL3-BAG3-Nicking/PegRNA-PGK-puroR质粒图谱载体图谱和pGL3-BAG3-Nicking/PegRNA-PGK-puroR载体序列质粒序列多克隆位点信息
pGL3-BAG3-Nicking/PegRNA-PGK-puroR质粒载体简介
pGL3-BAG3-Nicking/PegRNA-PGK-puroR质粒序列载体序列
LOCUS pGL3-BAG3-Nicking/PegRNA-PGK-puroR 4944 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4944)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4944
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(31..54)
/label=OL459
primer_bind 48..125
/label=OL409
misc_feature 51..125
/label=hCtRNA
primer_bind complement(51..125)
/label=OL410
primer_bind 66..89
/label=OL464
primer_bind complement(108..126)
/label=OL465
misc_feature 126..145
/label=BAG3-nicking
primer_bind 126..141
/label=OL301
primer_bind 145..186
/label=OL462
misc_RNA 146..221
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(204..240)
/label=OL463
misc_feature 222..296
/label=hCtRNA
primer_bind 222..296
/label=OL409
primer_bind complement(222..296)
/label=OL410
primer_bind 237..260
/label=OL464
primer_bind complement(279..296)
/label=OL465
primer_bind 294..312
/label=OL209
misc_feature 297..316
/label=BAG3 spacer
misc_RNA 317..392
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 317..357
/label=OL462
primer_bind complement(375..393)
/label=OL463
misc_feature 393..404
/label=RTT
misc_feature 405..419
/label=PBS
primer_bind complement(406..419)
/label=OL209
primer_bind 425..444
/label=OL458
misc_feature 463..580
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 629..1139
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 1161..1760
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 1907..2028
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2447..3035)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3206..4066)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4067..4171)
/gene="bla"
/label=AmpR promoter
rep_origin 4198..4653
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4784..4832
/note="synthetic polyadenylation signal"
misc_feature 4846..4937
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta
61 tagctcagtg gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc
121 ccccttctcc ttacctcagg tccgtgtttt agagctagaa atagcaagtt aaaataaggc
181 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt
241 ggtagagcat ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctactc
301 agccagataa acagtggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
361 atcaacttga aaaagtggca ccgagtcggt gcacctgtcc acgctgttta tctggctgat
421 ttttttctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
481 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
541 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
601 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
661 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
721 accctgggac tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
781 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
841 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
901 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
961 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
1021 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
1081 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
1141 gggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
1201 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
1261 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
1321 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
1381 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
1441 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
1501 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
1561 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
1621 ccggggtgcc cgccttcctg gaaacctccg cgccccgcaa cctccccttc tacgagcggc
1681 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
1741 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
1801 cacgacccca tgcatcggta cctttaagac caatgactta caaggcagct gtagatctta
1861 gccactttct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
1921 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
1981 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
2041 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
2101 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
2161 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
2221 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
2281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
2341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
2401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
2521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
2581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
2641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
2701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
2761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
2821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
2881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
2941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
3121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
3181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
3361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
3541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
3601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
3661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
3721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
3781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
3841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
3901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
3961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
4021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
4081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
4141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4321 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4381 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
4441 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4501 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
4561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4621 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact
4681 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat
4741 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt
4801 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca
4861 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg
4921 tgccagaaca tttctctatc gata
//
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