| 质粒名称: | pAAVLINK-EF1alpha-5'-NRXN3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001823 |
| 产品用途: | 5' AAVLINK plasmid for NRXN3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-5'-NRXN3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-5'-NRXN3载体质粒基本信息
pAAVLINK-EF1alpha-5'-NRXN3质粒图谱载体图谱和pAAVLINK-EF1alpha-5'-NRXN3载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-5'-NRXN3质粒载体简介
pAAVLINK-EF1alpha-5'-NRXN3质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-5'-NRXN3 5874 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5874)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 9, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5874
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3014
/codon_start=1
/gene="NRXN3"
/gene_synonym="C14orf60"
/product="neurexin 3 isoform 5"
/label=NRXN3
/note="isoform 5 is encoded by transcript variant
7;neurexin III; neurexin-3-alpha"
/db_xref="GI:1056849789"
/db_xref="CCDS:CCDS81831.1"
/db_xref="GeneID:9369"
/db_xref="HGNC:HGNC:8010"
/db_xref="MIM:600567"
/protein_id="NP_001317124.1"
/translation="MSSTLHSVFFTLKVSILLGSLLGLCLGLEFMGLPNQWARYLRWDA
STRSDLSFQFKTNVSTGLLLYLDDGGVCDFLCLSLVDGRVQLRFSMDCAETAVLSNKQV
NDSSWHFLMVSRDRLRTVLMLDGEGQSGELQPQRPYMDVVSDLFLGGVPTDIRPSALTL
DGVQAMPGFKGLILDLKYGNSEPRLLGSRGVQMDAEGPCDERPCENGGICFLLDGHPTC
DCSTTGYGGKLCSEDVSQDPGLSHLMMSEQAREENVATFRGSEYLCYDLSQNPIQSSSD
EITLSFKTWQRNGLILHTGKSADYVNLALKDGAVSLVINLGSGAFEAIVEPVNGKFNDN
AWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSN
NFMGCLKEVVYKNNDIRLELSRLARIADTKMKIYGEVVFKCENVATLDPINFETPEAYI
SLPKWNTKRMGSISFDFRTTEPNGLILFTHGKPQERKDARSQKNTKVDFFAVELLDGNL
YLLLDMGSGTIKVKATQKKANDGEWYHVDIQRDGRSGTISVNSRRTPFTASGESEILDL
EGDMYLGGLPENRAGLILPTELWTAMLNYGYVGCIRDLFIDGRSKNIRQLAEMQNAAGV
KSSCSRMSAKQCDSYPCKNNAVCKDGWNRFICDCTGTGYWGRTCEREASILSYDGSMYM
KIIMPMVMHTEAEDVSFRFMSQRAYGLLVATTSRDSADTLRLELDGGRVKLMVNLDCIR
INCNSSKGPETLYA"
misc_feature 1861..1866
/label=SDS
misc_feature 2622..2627
/label=SDS
intron 3015..3055
/label=Splicing Donor
misc_feature 3059..3092
/label=lox2272-JT1571
repeat_region 3137..3277
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3137..3266
/label=AAV2 ITR
repeat_region 3138..3278
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3352..3807
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4089..4193
/gene="bla"
/label=AmpR promoter
CDS 4194..5054
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5225..5813
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgagctc
721 cacactccac tcggttttct tcaccctgaa ggtcagcatc ctgctggggt ccctgctggg
781 gctctgcctg ggccttgagt tcatgggcct ccccaaccag tgggcccgct acctccgctg
841 ggatgccagc acacgcagtg acctgagttt ccagttcaag accaacgtct ctacggggct
901 gctcctctac ctggatgatg gcggcgtctg cgacttccta tgcctctccc tggtggatgg
961 ccgcgttcag ctccgcttca gcatggactg tgccgagact gccgtgctgt ccaacaagca
1021 ggtgaatgac agcagctggc acttcctcat ggtgagccgt gaccgcctgc gcacggtgct
1081 gatgcttgat ggcgagggcc agtctgggga gctgcagccc cagcggccct acatggatgt
1141 ggtcagtgac ttgttccttg gtggagtccc tactgacata cgaccttctg ccctgaccct
1201 tgatggagtt caggccatgc ccggcttcaa ggggttaatt ctggatctca agtatggaaa
1261 ctcggagcct cggcttctgg ggagccgggg tgtccagatg gatgccgagg gaccctgtga
1321 tgagcgtccc tgtgaaaatg gtgggatctg ctttctcctg gacggccacc ccacctgtga
1381 ctgttctacc actggctatg gtggcaagct ctgctcagaa gatgtcagtc aagatccagg
1441 cctctcccac ctcatgatga gtgaacaagc tcgagaggag aatgtggcca ctttccgagg
1501 ctcagagtat ctgtgctacg acctgtctca gaacccgatc cagagcagca gtgatgaaat
1561 caccctctcc tttaagacct ggcagcgtaa cggcctcatc ctgcacacgg gcaagtcggc
1621 tgactatgtc aacctggctc tgaaggatgg tgcggtctcc ttggtcatta acctggggtc
1681 cggggccttt gaggccattg tggagccagt gaatggaaaa ttcaacgaca acgcctggca
1741 tgatgtcaaa gtgacacgca acctccggca ggtgacaatc tctgtggatg gcattcttac
1801 cacgacgggc tacactcaag aggactatac catgctgggc tcggacgact tcttctatgt
1861 aggaggaagc ccaagtaccg ctgacttgcc tggctcccct gtcagcaaca acttcatggg
1921 ctgccttaaa gaggttgttt ataagaataa tgacatccgt ctggagctgt ctcgcctggc
1981 ccggattgcg gacaccaaga tgaaaatcta tggcgaagtt gtgtttaagt gtgagaatgt
2041 ggccacactg gaccccatca actttgagac cccagaggct tacatcagct tgcccaagtg
2101 gaacactaaa cgtatgggct ccatctcctt tgacttccgc accacagagc ccaatggcct
2161 gatcctcttc actcatggaa agccccaaga gaggaaggat gctcggagcc agaagaatac
2221 aaaagtagac ttctttgccg tggaactcct cgatggcaac ctgtacttgc tgcttgacat
2281 gggctctggc accatcaaag tgaaagccac tcagaagaaa gccaatgatg gggaatggta
2341 ccatgtggac attcagcgag atggcagatc aggtactata tcagtgaaca gcaggcgcac
2401 gccattcacc gccagtgggg agagcgagat cctggacctg gaaggagaca tgtacctggg
2461 agggctgccg gagaaccgtg ctggccttat tctccccacc gagctgtgga ctgccatgct
2521 caactatggc tacgtgggct gcatccgcga cctattcatt gatgggcgca gcaagaacat
2581 tcgacagctg gcagagatgc agaatgctgc gggtgtcaag tcctcctgtt cacggatgag
2641 tgccaagcag tgtgacagct acccctgcaa gaataatgct gtgtgcaagg acggctggaa
2701 ccgcttcatc tgcgactgca ccggcaccgg atactgggga agaacctgcg aaagggaggc
2761 atccatcctg agctatgatg gtagcatgta catgaagatc atcatgccca tggtcatgca
2821 tactgaggca gaggatgtgt ccttccgctt catgtcccag cgagcttatg ggctgctggt
2881 ggctacgacc tccagggact ctgccgacac cctgcgtctg gagctggatg gggggcgtgt
2941 caagctcatg gttaacttag actgtatcag gataaactgt aactccagca aaggaccaga
3001 gaccttgtat gcaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
3061 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
3121 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
3181 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
3241 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
3301 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3361 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3421 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3481 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
3541 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
3601 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
3661 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
3721 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
3781 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
3841 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3961 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4021 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
4081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4381 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
5761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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