pCMV-Xist-PA

LOCUS       pCMV-Xist-PA               18958 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Xist-PA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18958)
  AUTHORS   Wutz A, Jaenisch R
  TITLE     A shift from reversible to irreversible X inactivation is triggered 
            during ES cell differentiation.
  JOURNAL   Mol Cell. 2000 Apr . 5(4):695-705.
  PUBMED    10882105
REFERENCE   2  (bases 1 to 18958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..18958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             15381..15614
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     intron          15654..16226
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(16358..16377)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    16423..16478
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(16477..16496)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(16876..16893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17047..17635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17127..17146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17806..18666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18429..18448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18667..18771)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     18839..18857
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggatcagtt aaaggcgtgc aacggcttgc tccagccatg tttgctcgtt
      721 tcccgtggat gtgcggttct tccgtggttt ctctccatct aaggagcttt gggggaacat
      781 ttttagttcc cctaccacca agccttatgg cttatttaag aaaacatatc aaaattccac
      841 gagatttttg acgttttgat atgttctggt aagatttttt ttttgacatg tcctccatac
      901 tttttgatat ttgtaatatt ttcagtcaat ttttcatttt taaggaatat ttctttgttg
      961 tgccttttgg ttgatacttg tgtgtgtatg gtggacttac ctttctttca ttgtttatat
     1021 attcttgccc atcggggcca cggatacctg tgtgtcctcc ccgccattcc atgcccaacg
     1081 gggttttgga tacttacctg ccttttcatt cttttttttt cttattattt ttttttctaa
     1141 acttgcccat ctgggctgtg gatacctgct tttattcttt ttttcttctc cttagcccna
     1201 tcggggccat ggatacctgc tttttgtaaa aaaaaaaaaa aaaaaaaaaa acctttctcg
     1261 gtccatcggg acctcggata cctgcgttta gtcttttttt cccatgccca acggggcctc
     1321 ggatacctgc tgttattatt tttttttctt tttcttttgc ccatcggggc tgtggatacc
     1381 tgctttaaat tttttttttc acggcccaac ggggcgcttg gtggatggaa atatggtttt
     1441 gtgagttatt gcactacctg gaatatctat gcctcttatt tgcgtgtact gttgctgctg
     1501 atcgtttggt gctgtgtgag tgaacctatg gcttagaaaa acgactttgc tcttaaactg
     1561 agtgggtgtt cagggcgtgg agagcccgcg tccgccatta tggcttctgc gtgatacggc
     1621 tattctcgag ccagttacgc caagaattag gacaccgagg agcacagcgg actggataaa
     1681 agcaaccaat tgcgctgcgc tagctaaagg ctttctttat atgtgcgggg ttgcgggatt
     1741 cgccttgatt tgtggtagca tttgcggggt tgtgctagcc ggaagtagaa agccaaggag
     1801 tgctcgtatt agtgtgcggt gttgcgcgga agccgcagag gactagggga tagggctcag
     1861 cgtgggtgtg gggattgggc agggtgtgtg tgcatatgga cccctggcgc ggtcccccgt
     1921 ggctttaagg gctgctcaga agtctataaa atggcggctc gggggctcca cccgaggctc
     1981 gacagcccaa tctttgttct ggtgtgtagc aatggattat aggacattta ggtcgtacag
     2041 gaaaagatgg cggctcaagt tcttggtgcg gtataacgca aagggctttg tgtgtcacat
     2101 gtcagcttca tgtctgagtt agcctggaga ggtggcacat gctcttgaat gtgtctaaga
     2161 tggcggaagt catgtgacct gccctctagt ggtttctttc agtgattttt tttttggcgg
     2221 gctttagcta cttggcgggc tttgcccgag ggtacacttg gtgcattatg gtagggtgtg
     2281 gttggtccta ccttgtgcca ctcgaagctg aggcaaggct aagtggaagt gttggttgcc
     2341 acttgacgta actcgtcaga aatgggcaca agtgtgaaag tgttggtgtt tgcttgactt
     2401 ccagttagaa atgtgcatta ttgcttggtg gccaggatgg aattagactg tgatgagtca
     2461 ctgtcccata aggacgtgag tttcgcttgg tacttcacgt gtgtctttag tcatcatttt
     2521 ttcgaagtgc ctgcccaggt cgggagagcg catgcttgca attctaacac tgaagtgttg
     2581 gatgatgtcg gatccgattc gagagaccga ggctgcgggt tcttggtcga tgtaaatcat
     2641 tgaaacctca cctattaaaa gaaagaaaag tatctaaggc catttcaagg acatttgact
     2701 catccgcttg cgttcatagt ctcttacagt gctctatacg tggcggtgca aactaaaact
     2761 cagcccgttc cattcctttg tattgttcag tggctagtct acttacacct tggcctctga
     2821 tttagccagc actgatctca agcggttctc taagcctact gggtataagt ggtgactttg
     2881 gccagagtca tagtggatca caaatcactg gtgaagaggt agaatcctac cttcttccaa
     2941 aatctacccc atgactattg ctggggttgc attttgattt caatgaatat tttggatgcc
     3001 aacgacacgt ctgatagtgt gctttgctag tgtttgaatt taaaaccgaa gtgattgttt
     3061 tcaaaatgta tttacggatt tgcttacttg ttgaattcat tttaattacc tttagtgaat
     3121 tgttactttg gagtccttaa agttttcaat aatttttttg gcagatgata ctcaaattac
     3181 ttggcactta aatgtacttt ctttcaaact catccaccga gctactcttc aaatttttaa
     3241 gtcttataac acagatactg ttaatgtaaa gtgaacatta tgactggatg tcaggagtat
     3301 ttgaggttct ataccagttc aggctttgct tttgttgcta ttgttgatgc tatattgact
     3361 aatggtttta cttgtcagca agagccttga attgtaatgc tctgtgtcct ctatcagact
     3421 tactgttata atagtaatat taaggcctac atttcaactt tctgtgtgtt cttgccttta
     3481 tggcatctag attctcctca agactcagca aatagtgctg ctgctattgc tgccccagcc
     3541 ccaggcccag ccccagcccc tgccccagcc ccagccccag cccctgcccc agccccagcc
     3601 cctgcccctg ccccagcccc tgccccagcc ccagccccag cccctacccc tgcccctgcc
     3661 cctgccccac ccaaccaacc caatccagtc cagccctgcc ccagcccagt cctagcccca
     3721 ggcccagata ctttcagacc tatcccaagc ccacttctac ttagagaaat tcgaatcttc
     3781 attgattcag tgctaaaatg cagtgtccat cactcagcct ataagactga gacagcccat
     3841 ctataccccc tccatactga cttctagagt catggaattt cacttaatgc atagaatcgt
     3901 attgctaaaa tgcagtgccc atcactcagc ctataagact gagatagccc atctataccc
     3961 cctccatact gacttacaga gtcatggagt ttcacttaat gcatgcagtc ctattgctaa
     4021 aatgcagtgc ccataactca gcctataaga ctgagatagc ccatttatac cccatacccc
     4081 ctccatactg acttctaggg tcatggaatt tcacttaata catagaatcg tattgctaaa
     4141 atgcagtgtc catcactcag tctataagac tgagatatcc ctatgtatac cccatactcc
     4201 ctccatactg acttccagag tcatagaatt tcactttgca tacggtccta ttgctaaaat
     4261 gcagtgtcca tcactcagtc tataagactg agatatccct atgtataccc catactccct
     4321 ccatactgac ttccagagtc atagaatttc actttgcata cggtcctatt gctaaaatgc
     4381 agtgcccatc actcagccta taagactgag atagcccatc tataccccct ccatactgac
     4441 ttccagagtc atggaatttc acttaatgca tgcagtccta ttgctaaaat gcagtgccca
     4501 tcactcagcc tataagactg agatagccca tctatacccc ataccccctc catactgact
     4561 tccagagtca tggaatttca cttaatgcat gcagtcctat tgctaaaatg cagtgcccat
     4621 cactcagcct ataagactga gatagcccat ctatacccac tccatactga cttccagagt
     4681 catggaattt cacttaatgc atgcagtcct attgctaaaa tgcagtgccc atcactcagc
     4741 ctataagact gagatagccc atctataccc actccatact gacttccaga gtcatggagt
     4801 ttcacttaat gcatgcagtc ctattgctaa aatgcagtgc ccataactca gcctataaga
     4861 ctgagatagc ccatttatac cccatacccc ctccatactg acttctaggg tcatggaatt
     4921 tcacttaatg catagaatcg tattgctaaa atgcagtgtc cattactcag cctataagac
     4981 tgagatatcc ctatgtatac cccatacccc ctccatactg acttccagag acatagaatt
     5041 tcactttgca tacggtccta ttgctaaaat gcagtgccca tcactcagcc tataagactg
     5101 agatatccct atctataccc tctaccccct ccatactgac ttccagagtc atggaatttc
     5161 acataatgta tagatttcta ttgctaaaat gcagtgccca taactcagcc tataagactg
     5221 agatagccca tctatacccc ctccatactg agttccagag tcatggaatt tcacttaatg
     5281 catagaatcg tattgctaaa atgcagtgcc catcactcag cctataagac tgagcccatc
     5341 tataccccat accccctcca tactgacttc cagagtcatg gaatttcact ttgcatacag
     5401 tcctacttta cttgtccatg gacaagtaaa caaagaactc ttgtccttca tgttaatcaa
     5461 gatacaccaa tcaaacaaga gttttatatc agagacttgc catggaggta tcatctctca
     5521 agtctccttt cctttaagga aagaaaacca ttctgtcatt gctgtagtag tcacagtccc
     5581 aagtttctaa gcagtgttca gtcgtctttt ctcatgtatt accttgagta ctgaataatt
     5641 ctgtcagaaa tattttgtcc attggattag actttagcta gtccagccct gtgtgcattt
     5701 agcaaagggg caaacacagg tctgttatca gacagttaaa gtgctcagtc ccaattttca
     5761 aggcattggc cattaaaggg ggtagaatac tatatactgt tggcatgctg tcatgggtgc
     5821 tatcgcccca ggtcacatct ttctaactga tggagataca tttatttgct catgatattg
     5881 tatactagtc tcacatgctt tcttatttca gccaaaaacc tctgcactgg aacattttat
     5941 gtggataatc ctgactagga attgagtctt ttctcaaggt cctaatacta cccttgcttt
     6001 atgtaaagag ggtgctgatt acttaatgcc tcttacacaa ttgtgcaaaa ttgcagttgt
     6061 tcaagtcccc ttctgttagt aaccaagatc ccataccctc ataccctaat gggtgacaat
     6121 caagggtgcc aaccaatgag accacttctc tgttctggtc tttctgctgt gctggggaat
     6181 caaaccttga gtcttgtgta cgctagtaaa gcactgtcat agagctacag ccccaccgtg
     6241 tggtggtttg agagaacagc ctcttatgta gcctgggctg ggcgggactt acaggcattg
     6301 ccacctgtaa tgtaaacata tttgtgcctg ttgtgtgcac agctgcattt gtccctcttc
     6361 ctaagcattg gataaagaaa ccaaactaag tcaagtcatt ttgttggtaa tcaagaagac
     6421 ctttgatctg tcctgttttt aacttccagg ctggcctgga acttagcata taacccaggc
     6481 tagccttgag ctcaggatct agcctgcgtt taacaagtgt tggcatatct ggttcctacc
     6541 actatgccct gcatgcagtc tttcatattg tgaatgtgca tatgtcattt cactgtagta
     6601 atctgcatct ggtgaagact tatttgtatt gcagcagtat ttaagatcct taacatagta
     6661 aatgtgcaca gtgttaactc tattgtacat attctcatgt ccacagttgt gccttttaga
     6721 tcaggactcc tgtacttagc aaagcaaaga ggctcactaa tataaagctt ctttcatgag
     6781 actatagatt gaaacgattc caatacggtc aatggtcctt caaggtaaga cttctgtctc
     6841 tgatcattca tatcctcttt gctttatgga attatgtatg tgctgtgcac ttgaaacccc
     6901 ttcctcaaac tatttatgta catactggca attttagtag gatcaatttt actcttaact
     6961 ttgaagtaca gaagtggtgt tgacctataa ggtcccattt tgtggcttgc taataataat
     7021 gactgattgt agtaggcctt ttctgttcac tacagaagga aacctgaaca gcgtaaaact
     7081 gtaatggcca taaacatgta ccttgcatat tagtatgcat ttactgcaca catctcattc
     7141 catttggata cgatcctact ctcaaaccct tttgcagtac agcaagggtc actaatcttt
     7201 tggcttcttc atcttcctgg acactggata aggctgtccc ctcctttcca ctctttaatt
     7261 tccaggacta ttactttaaa gacttaatat ttgcataaag gatggggttt ttaattgata
     7321 acatgtccct tgaacattaa tgtatataac agggacatga tccattcatt ttaataaaaa
     7381 tacttggcca gttaatgtgt aaaattacac ttatccacaa ccttattact tttcggacca
     7441 ttgtatctct tgcactcctg caagggatac cgtttatctc ccaaggtccc tgctagtgga
     7501 ccattaatat acagtgaatc ttcctttgtc tttgccagta aacaaaggcc atactccttc
     7561 gcctttcatt tgcactatat caggatatgc tgatcaacaa ggccgcattc ttttggactg
     7621 ttatcatata ttaaatgtat gcgtatgcac tgccacctgc tctgtgcact tgaaaggatc
     7681 ccactcactt ccttagcacc ttcagcagga agtgataata agctcaagac tttcatttgg
     7741 aaagttcaca tgtctaagca cttctctaag aactactgta ccctcttctc cgctttaaag
     7801 cagaaagagg gttgtacgaa gtgctcttca tttggactta agtgcattaa tgcagttagt
     7861 tgtccatcat tacctttgga gttggatttt acatccttgt actcttttga caccagaggc
     7921 atattaatta tttctgagca cttctcttgt caatattaat ctgtaccctt acacatatga
     7981 cctgtgcggc agcaaaggtt ctgaaatgcc taccttttga ctggggctgc tgagtggtag
     8041 taactattag taacctcagc atttggatga ttactatgca aaaatgtcaa ggacctgtgt
     8101 gctctctttg cataccatca aggctactga gtcccagaat taattgctaa gttatgcgta
     8161 tttataacta tgaatgtctg gaatattttg tcccctttac attattgcag aggttgctga
     8221 gcccccgaaa ctacccggta ctgtcaatga gcacaggggc tctgacgaat gacctgctct
     8281 cttccttaaa ctgattttgg gactcttaat aggcacaatg gcagttctgg atggtttatt
     8341 ttctactcca acttgagcaa atcccctgct agtttcccaa tgatataata aagtacagca
     8401 gtatgtacac ccaacaatga cccggatttc gacccttttt gcattgcttt aatatataca
     8461 atcctaaata gtcacaatct cacactttat agtgttcctt ttgcccggcc tctagtttgt
     8521 ccattgacca cttttctgaa tcactaattc tcacaaaccc atcattaagg aagagtttgt
     8581 gccctttctc aattccatca tgccatccct tttgcctctt tgtttgaaca gtattgactg
     8641 ggcaaagccc ttctcttgac ttaaagtcaa caacaccagt ttactcactt catatggcta
     8701 cagtgtctca gttgccttct ccttgctccc actgaacaga gacacctcga attcttacat
     8761 tattctgggt aatgttaatt accccaaaca ccctatgtgt cattaataaa ttttggtgta
     8821 tttatacact gaatagcaaa agcaggccaa aactaggtgg atgagccttc aatctttaac
     8881 ttgcacttct aaattattcc aattccaact gctggcacat tctagggcca ggaaccattc
     8941 ttgcctacct ttattaatgc tttattgtgc aaaatattgc aggcaagtag ctcagggagt
     9001 tggattgcca ccttttactt ggggctttcc tttacagtat gaactgaaaa ttgtcttcct
     9061 gagaaggaag cttagcactt ttctttccat tcttcctcca ggaaggagcc aactgtctgc
     9121 ttaagaaact ttaagcccga ttttgtatat tgctactgta caggaccaac tgccagaaaa
     9181 gttattgata attttattcc ttaagaaagg catttggatt gcaaggtgga ttgactgtga
     9241 gatcattagc ttttgtgaag taaaaatagc catttgtgtc atgtttctga agactaagca
     9301 gtgtctcagt gtactgaggg tgatgagtct gtggaaagat cagtgcaact attgcagaat
     9361 gtttaagaca agtatctttg cttggtcttt actacaagtt taacaaaacg aaaaagtcaa
     9421 tctttgtgtg gcctttagta tgattaactt tttggaagat gacctaagcc ttctaatcat
     9481 tatattttgt ctgacattgg tcaccagtcc ttgcttattt ttaaaaggtg actggatgga
     9541 ttaaatttga gaacatgtca agtcgccttt gaaaattata taggccatca catttaatta
     9601 attcattcta tccaccatta aactctggca ataatttgaa gtagcttgaa aattcctaaa
     9661 gtgggaattt attttagaga tgatagaacc tgtttcccca ctttacattt taaaatatgt
     9721 ctgccaggat ctaatcattc ctttaaacgt acacttcaaa gagagatttt cctagtaaga
     9781 aaagagcttt ctctagtgtg aagggtgctt tgtagccgcc gagtacttag gtcttttttg
     9841 ggagctattg tgtatgagtg tatgtatgtg tgtgtgtaca tgcatgttgc tgcgcgcagt
     9901 cattcattca catggtgctc agacaacaat gggagctggt tcgtctatct tgtgggtcct
     9961 ggagatcaaa gtgagatcat caggcttggc agcaagtgcc ttcaccctcc gcgtgccatc
    10021 ttgccatccc gctgctgagt gtttgatatg acattgctga tgaaaataat catcacaaca
    10081 gcagttctcc cggcattact gagaaatgat actatttttc tgaggaggat gttcaagtaa
    10141 ctcatccagt gcaggatcct gcttgaacta ctgctcctcc gttacatcag actctggctg
    10201 tttagactac aggatgaatt tggagtctgt tttgtgctcc tgcctcaaga agaaggattg
    10261 cctggattta gaggagtgaa gagtgctgga gagagcccaa agggacaaac aatccctatg
    10321 tgagattcaa ggactgccag cagcctatac agctacatta catctcagca gaacttctct
    10381 tcaagtcctc gctactctga acaaaaagct tacaggccac atggagaaaa aaagatctcc
    10441 ccccagaatt gtgggcttgt tgctttgcag tgctggcgac ctattccctt tgacgatccc
    10501 taggtggaga tggggcatga ggatcctcca ggggaatagc tcaccaccac tgggcaacag
    10561 gcctagccca gatttcagtg agacgctttc ctgaacccag caaggaagac aaaggctcaa
    10621 agaatgccac cctacatcaa agtaggagaa aagctgctgc aatagtggca ctgaccttcc
    10681 gaggaagcca ttctgctcta tttggttctc tctccagaag ttaggagaac tttgccagct
    10741 gtttacatac ttcaagatgc actgctaccc tactcatgcc atataataca caatgccatc
    10801 taccaaatat tacccttccc caaagcagca cagaaaactg ggtcttcagc atgatcaagc
    10861 aatgtgaaca cacaaaagga aggcagcttt ataaatgacc cgaggatcaa catgcctgac
    10921 tgcagcatct taaaagcaat agaatgagtg tgtattgtgt tgtgtgtcta tttcttgttt
    10981 tatgtatcta ttttttcctt ggtctgtgtg tctaattctt tgttacacct atttcttcct
    11041 tgctttgtgt gtctatttct tccttgcatt gtgtctaatt ctttgttata tctatttctt
    11101 ccttgcttcg tgtgtctgtc ttccttgctt tgtgtctatt tcttccttgc agttgtgtct
    11161 aattctttgt tacatctatt tcttccttgc ttttgtgtgt ctttctttct tgcttttgtg
    11221 tgtctatttc ttccttgcag ttgtgtctaa ttctttgtta catctatttc ttccttgctt
    11281 ttgtgtctat ttcttccttg cattgtgtct aattctttgg catatatatt tcttcattgc
    11341 tttgtgtgtc tatgtctcct tgtgttgtct aattcgttgt tgcatctatt tcttccttgc
    11401 tttgtgtcta tgtcttcctt gttttgtgta tctacttctt ccttgtgtgt ctaattcttt
    11461 gttacatcta tttcttcctt cctttgcatg tctccttctt tcctttgtgt gtcttttctg
    11521 tctgcagtgt gccttaccta ttcccatgtt tctcctgcat gttctttctt gcagagcttt
    11581 gagctttgtt tcactttctc tggtgcctgt gtggtctgct ttgtcttcac tagctatggc
    11641 tctctgtttt atctatctga ttgctatttc tcttagcttt tcttttactc ctgcctttcg
    11701 tgactcccct ttgggtcaca tgttgcatgc atccctctct ttttcttgtg ctcaccccac
    11761 ttgttctttg ttcaagttct ctttgtcagt ccatttcagt tttctttctg ctgcttctat
    11821 ccttagtgaa ttcttgttta catttcttcc ctgcctttct tgggccactt tctctgtttt
    11881 cttttgtatt tgtgtctctt tgctattggt ggatttctta tctcagcatc attctgttgc
    11941 tttgtgtttg cttgtgtttc tatcttctac tttcctcctt tctgttcact ttgagcattt
    12001 catctcttta caagtctgtg tctctcttgt attctaaagt aatcctttct tggatgtttc
    12061 tttgtatgta catgtgcgtg tgtgcatgtg tgttatgtgt gtcatgtgtg agaggagctt
    12121 catagcccct tcccaatagg tccagaatgt cacccgtgga gccgttcctc acaccagact
    12181 gccctgagaa ataatctaag acaaaataca tcattccgtc cggtcaggat tcaagtggct
    12241 ctgaagtgaa cgcccaagta gaagacagaa gttttgcgac ttgagattta aaaggaccaa
    12301 aatacacaga tggcccgtct tgagctggct ggacagaatg ctgacaaccc aaagaagagg
    12361 aactgtttct acaggacacc tgtgacttcc aagagcgggg aactacgtat gtcataagac
    12421 acaaaacctg agctaagtcc aagcataaga cctaaggacc caatcctata tggacagaat
    12481 atttaagaga taaaggccta tggcccagaa ctctggaagg atatttctat ccttctatcc
    12541 ccaagaccaa gaagggaaat tcgaagatga gacctgcccc ccaaccccag catccctttc
    12601 catttcttat atttctattt aagctgtctt cacttgagat gtaatttttc attgttgcca
    12661 ttgcccataa aggaatacgt ttttagctgg atagtattgt gcaagggtct gttttaaact
    12721 gggtcttagc catttgttaa attgttgatg ttttacaact tccatttctc ttcacatctg
    12781 ctccacttga gacggaacta aatccagcca gtgtatatag cctgactatt gaaacttccc
    12841 taggaataag catgcataca gatatgcata ctgccatcct ccctacctca gaagccctag
    12901 gctgacaaga aaaggaaagc atcaggttgt tagggggaaa acaatgtcag gctatctaga
    12961 gaaaatataa agagttgttc cagaccaatg agaagaatta gacaagcaat atgcagatgt
    13021 gccaaccctc tgagaagcac cagccagtgt caccttcttt ctttgggctt aggtgagcag
    13081 ggtatggttt tctaataatg gtttggggac aaaatgaggt ctgaactccc tgctcatagt
    13141 agtggccgag taatttggtg catttcacca aaggaactcc tgggtctaat acctaccttt
    13201 aaaattaatg atgagagact ctaaggacta cttaacgggc ttaatctttt tcgtgccttc
    13261 ctcttcctct gtaagaggga agttaaatga cacaggatga aaaagtaaca tgctcatagc
    13321 acattggcaa ttatacatgg ttattatctg aaagtgtaga gcttttccta taaggcatca
    13381 gactaagtac ctgaagcttt gtgggttcat ggtcttagtt gcatattcct tagttgcaaa
    13441 tccttttcaa aaggtaagaa aaaggcacac tggtctattg cctgtacttg atcaagccct
    13501 gatatgaatg ccagggaatg tctgagtaac attaatttcc ttccctgcat attttttgtg
    13561 ctgaatacta aggctgtgat gcttcactgt ggtcaccccc aggtaacaag atattaccag
    13621 gtaaccagga aacgtatgaa tacgtaaacc atgaagccta ctgtaacttc caagtcagtg
    13681 ctgagtatgt attacatagt agctgaagtc tacgcctctg tgtgctatag gcacaaagat
    13741 tgctctagga ataacatgct ttgtaaaaac aaatatatga acataacggg gcttgaatga
    13801 ataacagtcc atatacttaa ggccagtgtg tttcttctgc tttggtgagg ctcagtaagt
    13861 tatattatac caggtagcag aagagaaaac acatggaaac tgattttaaa ctacaaacta
    13921 ggtcactaat gcaggtgatt gattacccta ttctgatcac cttctaattt ctgaataccc
    13981 atgttcagca ctgggaataa caaaggggga cattaccaca gaactagaat ttacaaaaga
    14041 atgcattaaa taaagcatta tacagctatc aattgttcca tgtgtgcaaa tgaatgacta
    14101 ctaactacct ctgatgtatc cgatattgtt ttgggtacat gaaatattca tgagtaactg
    14161 ccatgaaata agaatgtttg cattccatac tattcataag gaatgagcca atgcttaatt
    14221 taatcagtca aaacttgagt gataagggca tgttaataca agaacatttg cccaggtcac
    14281 attatggttg tgggtacttt cttaactata aagcagttca gtagtataag acaagacaaa
    14341 ttttctatag aaataaagct gcctataaaa taggcatagt ctctacaaaa ttttcattgt
    14401 actttttagc ccataatggg aagagtacag ttaacaagct gggtgtggta gcatgtgctc
    14461 tgagctgaag caacaggacc acttgagccc agaaattgga ggctagcctg ggaagaccat
    14521 aaggtcaatc tcaaacctgg aggctaaata ttgtctccca tgtgtatatt ctctttcatg
    14581 ggtactggag agatacacag acgtacattt cagtgtgtcc acacttgaga ataatatgta
    14641 cgttggcatt ttatgaactc ggaggtacca tataaatgta acaattcatt ttcttacttg
    14701 gtatcaattt ccaggctttt aaaattctgc cacatttatt atactgtgaa aataaagtaa
    14761 ataagtaact gtgaaccact gaatatatga agcattcaat acttgatgag tacatactga
    14821 atggcagtca tttattacaa aacagtgccc ttgctaggca ctgggatgca aagagcattc
    14881 tcattgtcct gtgtatctaa agaaattatg catgagatta atttataatt tgtaaactgc
    14941 catatatatg tgtatatatg caatatttgc ctggtgtgca atgactttgc ttttatccca
    15001 ggcatgcaca acagatctgt gtggagcttt gtgaagtcta cagttctata aagccgggac
    15061 ctaactgttg gctttatcag tgaacagtga ttactttcta agtttcataa tggctgaaac
    15121 ttaatcataa tgcttatcac ctaacaccac ctaataataa ttttaccatg ctatgtgttg
    15181 agcgaacaca tagattgctt tctagcatta tgtagcactt ataggagtga aatctagacc
    15241 aaaacttcaa ttcacttcaa tgaggaaatg aaaacagaaa aaaaaaatgg atttgtgcaa
    15301 ggcagtgtgc taaatgttac actgagtgga ctatgctgtc taggatactt cccagatcaa
    15361 gcttatcgat accgtcgacg gcccccccga ccgatgtcag cctgggggac gagctccact
    15421 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
    15481 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
    15541 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
    15601 acgagtacgg tgggtagggg gcgcgaggat cctctagagc tgagaacttc agggtgagtt
    15661 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg
    15721 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct
    15781 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt
    15841 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta
    15901 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag
    15961 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta
    16021 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg
    16081 gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata
    16141 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg
    16201 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc
    16261 atcattttgg caaagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
    16321 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
    16381 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
    16441 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
    16501 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
    16561 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
    16621 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
    16681 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
    16741 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
    16801 gatccctcga ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    16861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    16921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    16981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    17041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    17101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    17161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    17221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    17281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    17341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    17401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    17461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    17521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    17581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    17641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    17701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    17761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    17821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    17881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    17941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    18001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    18061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    18121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    18181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    18241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    18301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    18361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    18421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    18481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    18541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    18601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    18661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    18721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    18781 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    18841 taggcgtatc acgaggccct ttcgtcttca ctcgaggagc ttggcccatt gcatacgttg
    18901 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgtt
//