| 质粒名称: | pAAVLINK-EF1a-HA-5'-CREBBP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001721 |
| 产品用途: | 5' AAVLINK plasmid for CREBBP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CREBBP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CREBBP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CREBBP载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CREBBP质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CREBBP载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CREBBP质粒载体简介
pAAVLINK-EF1a-HA-5'-CREBBP质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CREBBP 7343 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7343)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7343
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4483
/codon_start=1
/label=CREBBP
/translation="MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELI
PNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQ
GQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLAT
SSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGA
GMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFS
QAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAI
ATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQ
AGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIG
SVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQ
MRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLST
IPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKK
VEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPV
IPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQM
NSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQ
PPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQH
TTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQP
QTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPG
PDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEV
DEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPE
SLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWL
YNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDA
AYYSYQNRYHFCEKCFTEIQ"
misc_feature 973..978
/label=SDS
misc_feature 1309..1314
/label=SDS
misc_feature 1531..1536
/label=SDS
misc_feature 2347..2352
/label=SDS
misc_feature 2492..2497
/label=SDS
misc_feature 3320..3325
/label=SDS
misc_feature 3614..3619
/label=SDS
misc_feature 3672..3677
/label=SDS
misc_feature 3816..3821
/label=SDS
intron 4484..4524
/label=Splicing Donor
misc_feature 4528..4561
/label=lox2272-JT1571
repeat_region 4606..4746
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4606..4735
/label=AAV2 ITR
rep_origin 4821..5276
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5558..5662
/gene="bla"
/label=AmpR promoter
CDS 5663..6523
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6694..7282
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggctgagaac ttgctggacg gaccgcccaa
781 ccccaaaaga gccaaactca gctcgcccgg tttctcggcg aatgacagca cagattttgg
841 atcattgttt gacttggaaa atgatcttcc tgatgagctg atacccaatg gaggagaatt
901 aggcctttta aacagtggga accttgttcc agatgctgct tccaaacata aacaactgtc
961 ggagcttcta cgaggaggca gcggctctag tatcaaccca ggaataggaa atgtgagcgc
1021 cagcagcccc gtgcagcagg gcctgggtgg ccaggctcaa gggcagccga acagtgctaa
1081 catggccagc ctcagtgcca tgggcaagag ccctctgagc cagggagatt cttcagcccc
1141 cagcctgcct aaacaggcag ccagcacctc tgggcccacc cccgctgcct cccaagcact
1201 gaatccgcaa gcacaaaagc aagtggggct ggcgactagc agccctgcca cgtcacagac
1261 tggacctggt atctgcatga atgctaactt taaccagacc cacccaggcc tcctcaatag
1321 taactctggc catagcttaa ttaatcaggc ttcacaaggg caggcgcaag tcatgaatgg
1381 atctcttggg gctgctggca gaggaagggg agctggaatg ccgtacccta ctccagccat
1441 gcagggcgcc tcgagcagcg tgctggctga gaccctaacg caggtttccc cgcaaatgac
1501 tggtcacgcg ggactgaaca ccgcacaggc aggaggcatg gccaagatgg gaataactgg
1561 gaacacaagt ccatttggac agccctttag tcaagctgga gggcagccaa tgggagccac
1621 tggagtgaac ccccagttag ccagcaaaca gagcatggtc aacagtttgc ccaccttccc
1681 tacagatatc aagaatactt cagtcaccaa cgtgccaaat atgtctcaga tgcaaacatc
1741 agtgggaatt gtacccacac aagcaattgc aacaggcccc actgcagatc ctgaaaaacg
1801 caaactgata cagcagcagc tggttctact gcttcatgct cataagtgtc agagacgaga
1861 gcaagcaaac ggagaggttc gggcctgctc gctcccgcat tgtcgaacca tgaaaaacgt
1921 tttgaatcac atgacgcatt gtcaggctgg gaaagcctgc caagttgccc attgtgcatc
1981 ttcacgacaa atcatctctc attggaagaa ctgcacacga catgactgtc ctgtttgcct
2041 ccctttgaaa aatgccagtg acaagcgaaa ccaacaaacc atcctggggt ctccagctag
2101 tggaattcaa aacacaattg gttctgttgg cacagggcaa cagaatgcca cttctttaag
2161 taacccaaat cccatagacc ccagctccat gcagcgagcc tatgctgctc tcggactccc
2221 ctacatgaac cagccccaga cgcagctgca gcctcaggtt cctggccagc aaccagcaca
2281 gcctcaaacc caccagcaga tgaggactct caaccccctg ggaaataatc caatgaacat
2341 tccagcagga ggaataacaa cagatcagca gcccccaaac ttgatttcag aatcagctct
2401 tccgacttcc ctgggggcca caaacccact gatgaacgat ggctccaact ctggtaacat
2461 tggaaccctc agcactatac caacagcagc tcctccttct agcaccggtg taaggaaagg
2521 ctggcacgaa catgtcactc aggacctgcg gagccatcta gtgcataaac tcgtccaagc
2581 catcttccca acacctgatc ccgcagctct aaaggatcgc cgcatggaaa acctggtagc
2641 ctatgctaag aaagtggaag gggacatgta cgagtctgcc aacagcaggg atgaatatta
2701 tcacttatta gcagagaaaa tctacaagat acaaaaagaa ctagaagaaa aacggaggtc
2761 gcgtttacat aaacaaggca tcttggggaa ccagccagcc ttaccagccc cgggggctca
2821 gccccctgtg attccacagg cacaacctgt gagacctcca aatggacccc tgtccctgcc
2881 agtgaatcgc atgcaagttt ctcaagggat gaattcattt aaccccatgt ccttggggaa
2941 cgtccagttg ccacaagcac ccatgggacc tcgtgcagcc tccccaatga accactctgt
3001 ccagatgaac agcatgggct cagtgccagg gatggccatt tctccttccc gaatgcctca
3061 gcctccgaac atgatgggtg cacacaccaa caacatgatg gcccaggcgc ccgctcagag
3121 ccagtttctg ccacagaacc agttcccgtc atccagcggg gcgatgagtg tgggcatggg
3181 gcagccgcca gcccaaacag gcgtgtcaca gggacaggtg cctggtgctg ctcttcctaa
3241 ccctctcaac atgctggggc ctcaggccag ccagctacct tgccctccag tgacacagtc
3301 accactgcac ccaacaccgc ctcctgcttc cacggctgct ggcatgccat ctctccagca
3361 cacgacacca cctgggatga ctcctcccca gccagcagct cccactcagc catcaactcc
3421 tgtgtcgtct tccgggcaga ctcccacccc gactcctggc tcagtgccca gtgctaccca
3481 aacccagagc acccctacag tccaggcagc agcccaggcc caggtgaccc cgcagcctca
3541 aaccccagtt cagcccccgt ctgtggctac ccctcagtca tcgcagcaac agccgacgcc
3601 tgtgcacgcc cagcctcctg gcacaccgct ttcccaggca gcagccagca ttgataacag
3661 agtccctacc ccctcctcgg tggccagcgc agaaaccaat tcccagcagc caggacctga
3721 cgtacctgtg ctggaaatga agacggagac ccaagcagag gacactgagc ccgatcctgg
3781 tgaatccaaa ggggagccca ggtctgagat gatggaggag gatttgcaag gagcttccca
3841 agttaaagaa gaaacagaca tagcagagca gaaatcagaa ccaatggaag tggatgaaaa
3901 gaaacctgaa gtgaaagtag aagttaaaga ggaagaagag agtagcagta acggcacagc
3961 ctctcagtca acatctcctt cgcagccgcg caaaaaaatc tttaaaccag aggagttacg
4021 ccaggccctc atgccaaccc tagaagcact gtatcgacag gacccagagt cattaccttt
4081 ccggcagcct gtagatcccc agctcctcgg aattccagac tattttgaca tcgtaaagaa
4141 tcccatggac ctctccacca tcaagcggaa gctggacaca gggcaatacc aagagccctg
4201 gcagtacgtg gacgacgtct ggctcatgtt caacaatgcc tggctctata atcgcaagac
4261 atcccgagtc tataagtttt gcagtaagct tgcagaggtc tttgagcagg aaattgaccc
4321 tgtcatgcag tcccttggat attgctgtgg acgcaagtat gagttttccc cacagacttt
4381 gtgctgctat gggaagcagc tgtgtaccat tcctcgcgat gctgcctact acagctatca
4441 gaataggtat catttctgtg agaagtgttt cacagagatc caggtaagta tcaaggttac
4501 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
4561 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
4621 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
4681 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
4741 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
4801 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4861 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
4921 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4981 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
5041 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
5101 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
5161 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
5221 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
5281 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
5341 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
5401 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
5461 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
5521 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
5581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
5641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
5701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
5761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
5821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
5881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
5941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
6001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
6061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
6121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
6181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
6241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
6301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
6361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
6421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
6481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
6541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
6601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
6661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
6721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6841 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
7021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
7081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
7141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
7201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
7261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
7321 ttgctggcct tttgctcaca tgt
//
