Lenti-mEGFP-BRE5

LOCUS       Lenti-mEGFP-BRE5               10477 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3497..5044
                     /codon_start=1
                     /label=BRE5
                     /translation="MGVTVQDICFAFLQNYYERMRTDPSKLAYFYASTAELTHTNYQSK
                     STNEKDDVLPTVKVTGRENINKFFSRNDAKVRSLKLKLDTIDFQYTGHLHKSILIMATG
                     EMFWTGTPVYKFCQTFILLPSSNGSTFDITNDIIRFISNSFKPYVLTDASLSQSNEENS
                     VSAVEEDKIRHESGVEKEKEKEKSPEISKPKAKKETVKDTTAPTESSTQEKPIVDHSQP
                     RAIPVTKESKIHTETVPSSTKGNHKQDEVSTEELGNVTKLNEKSHKAEKKAAPIKTKEG
                     SVEAINAVNNSSLPNGKEVSDEKPVPGGVKEAETEIKPIEPQVSDAKESGNNASTPSSS
                     PEPVANPPKMTWASKLMNENSDRISKNNTTVEYIRPETLPKKPTERKFEMGNRRDNASA
                     NSKNKKKPVFSTVNKDGFYPIYIRGTNGLREEKLRSALEKEFGKVMRITAADNFAVVDF
                     ETQKSQIDALEKKKKSIDGIEVCLERKTVKKPTSNNPPGIFTNGTRSHRKQPLKRKD"
     promoter        5055..5554
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5581..6375
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    6399..6987
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6870..6881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7195..7828
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7957..7973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7981..7997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8005..8035)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8050..8071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8359..8944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9115..9975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9976..10080)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10128..10262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcatgg gtgttaccgt gcaggatatt tgttttgcct ttctgcagaa
     3541 ttattacgaa cgcatgcgta ccgatccgag caaactggca tatttttatg ccagtaccgc
     3601 cgaactgacc cataccaatt atcagagcaa aagtaccaat gaaaaggatg atgtgctgcc
     3661 gaccgtgaaa gttaccggcc gcgaaaatat taataagttt ttcagccgca acgatgcaaa
     3721 agttcgtagt ctgaaactga aactggatac cattgatttt cagtataccg gtcatctgca
     3781 taaaagcatt ctgattatgg ccaccggtga aatgttttgg accggtaccc cggtgtataa
     3841 attttgtcag acctttattc tgctgccgag cagtaatggc agtacctttg atattaccaa
     3901 tgatattatc cgcttcatta gcaatagttt taagccgtat gttctgaccg atgccagtct
     3961 gagtcagagc aatgaagaaa atagcgttag cgccgttgaa gaagataaaa ttcgtcatga
     4021 aagtggcgtt gaaaaagaaa aagaaaagga aaaaagcccg gaaattagca aaccgaaagc
     4081 aaaaaaggaa accgtgaaag ataccaccgc cccgaccgaa agcagcaccc aggaaaaacc
     4141 gattgtggat catagccagc cgcgtgcaat tccggtgacc aaagaaagta aaattcatac
     4201 cgaaaccgtt ccgagcagca ccaaaggtaa tcataaacag gatgaagtta gcaccgaaga
     4261 actgggcaat gttaccaaac tgaatgaaaa aagccataaa gccgaaaaga aagcagcccc
     4321 gattaagacc aaagaaggta gcgttgaagc cattaatgca gtgaataata gtagtctgcc
     4381 gaatggcaaa gaagtgagtg atgaaaaacc ggttccgggt ggtgttaaag aagcagaaac
     4441 cgaaattaag ccgattgaac cgcaggtgag cgatgcaaaa gaaagcggca ataatgccag
     4501 taccccgagt agcagcccgg aaccggttgc aaatccgccg aaaatgacct gggcaagtaa
     4561 actgatgaat gaaaatagcg atcgcattag taaaaacaat accaccgttg aatacattcg
     4621 cccggaaacc ctgccgaaaa aaccgaccga acgcaaattt gaaatgggca atcgtcgtga
     4681 taatgccagt gccaatagta aaaataagaa aaaaccggtg ttcagtaccg tgaataagga
     4741 tggcttttat ccgatctata ttcgtggtac caatggcctg cgcgaagaaa aactgcgtag
     4801 tgcactggaa aaagaatttg gtaaagtgat gcgcattacc gccgcagata attttgccgt
     4861 ggtggatttt gaaacccaga aaagtcagat tgatgccctg gaaaagaaaa agaaaagtat
     4921 tgatggtatc gaggtgtgtc tggaacgtaa aaccgtgaaa aaaccgacaa gcaataatcc
     4981 gccgggtatt tttaccaatg gtacccgcag ccatcgtaaa cagccgctga aacgtaaaga
     5041 ttaagaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     5101 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     5161 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     5221 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     5281 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     5341 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     5401 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     5461 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     5521 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct tcctaggacc
     5581 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5641 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5701 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5761 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5821 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5881 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5941 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6001 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6061 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     6121 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6181 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6241 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6301 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6361 gacgagttct tctgacccgg gcggggcgcg tctggaacaa tcaacctctg gattacaaaa
     6421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6481 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6541 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6601 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6661 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6721 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6781 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6841 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6901 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6961 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     7021 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7081 agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa
     7141 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     7201 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7261 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7321 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7381 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7441 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7501 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7561 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7621 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7681 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7741 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7801 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7861 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     7921 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7981 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8761 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8881 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10081 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
    10141 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10201 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    10261 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    10321 ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    10381 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa
    10441 attgtatttg ttaaatatgt actacaaact tagtagt
//