pCDH-MTS-GFP-LplA

LOCUS       pCDH-MTS-GFP-LplA         8289 bp DNA     circular SYN 09-MAY-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    pCDH-MTS-GFP-LplA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8289
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2507..2599
                     /label=MTS
     CDS             2600..3313
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3344..4363
                     /label=LplA
     misc_feature    4370..4957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4841..4852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5030..5263
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5335..5456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5496..5631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5664..5680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5688..5704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5712..5742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5757..5778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6066..6654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6825..7685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7686..7790)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8264..8280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatgctgt ttaatctgag
     2521 gatcctgtta aacaatgcag cttttagaaa tggtcacaac ttcatggttc gaaattttcg
     2581 gtgtggacaa ccactacaag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2641 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2701 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2761 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2821 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2881 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2941 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3001 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3061 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3181 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3301 cgagctgtac aagtccggac tcagatctcg agctcaagct tccatgggct ccacattacg
     3361 cctgctcatc tctgactctt acgacccgtg gtttaacctg gcggtggaag agtgtatttt
     3421 tcgccaaatg cccgccacgc agcgcgttct gtttctctgg cgcaatgccg acacggtagt
     3481 aattggtcgc gcgcagaacc cgtggaaaga gtgtaatacc cggcggatgg aagaagataa
     3541 cgtccgcctg gcgcggcgca gtagcggtgg cggcgcggtg ttccacgatc tcggcaatac
     3601 ctgctttacc tttatggctg gcaagccgga gtacgataaa actatctcca cgtcgattgt
     3661 gctcaatgcg ctgaacgcgc tcggcgtcag cgccgaagcg tccggacgta acgatctggt
     3721 ggtgaaaacc gtcgaaggcg accgcaaagt ctcaggctcg gcctatcgcg aaaccaaaga
     3781 tcgcggcttc caccacggca ccttgctact caatgccgac ctcagccgcc tggcaaacta
     3841 tctcaatccg gataaaaaga aactggcggc gaaaggcatt acgtcggtac gttcccgcgt
     3901 gaccaacctc accgagctgt tgccggggat cacccatgag caggtttgcg aggccataac
     3961 cgaggccttt ttcgcccatt atggcgagcg cgtggaagcg gaaatcatct ccccgaacaa
     4021 aacgccagac ttgccaaact tcgccgaaac ctttgcccgc cagagtagct gggaatggaa
     4081 cttcggtcag gctccggcat tctcgcatct gctggatgaa cgctttacct ggggcggcgt
     4141 ggaactgcat ttcgacgttg aaaaaggcca tatcacccgc gcccaggtgt ttaccgacag
     4201 cctcaacccc gcgccgctgg aagccctcgc cggacgactg caaggctgcc tgtaccgcgc
     4261 agatatgctg caacaggagt gcgaagcgct gttggttgac ttcccggaac aggaaaaaga
     4321 gctacgggag ttatcggcat ggatggcggg ggctgtaagg tagggatcca atcaacctct
     4381 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4441 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4501 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4561 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4621 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4681 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4741 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4801 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4861 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     4921 tcagacgagt cggatctccc tttggccgcc tccccgccgg tacctttaag accaatgact
     4981 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5041 attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct ctggttagac
     5101 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5161 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5221 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5281 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     5341 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5401 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5461 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5521 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5581 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     5641 gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5761 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5821 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5881 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5941 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6001 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc ttaaaaaggc cgcgttgctg
     6061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7801 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7861 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     7921 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7981 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     8041 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     8101 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     8161 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8221 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     8281 gccaagctg
//