psPAX2-D64V-NC-MS2

LOCUS       psPAX2-D64V-NC-MS2               11108 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  MS2 coat protein-modified lentiviral packaging plasmid for packaging
            mRNA with an MS2 aptamer into lentivirus-like particles.
ACCESSION   .
VERSION     .
KEYWORDS    psPAX2-D64V-NC-MS2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun
            D, Yoo KW, Atala ZP, Lyu P, Atala A
  TITLE     Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for 
            transient expression and efficient genome editing.
  JOURNAL   Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 
            10.1093/nar/gkz093.
  PUBMED    30759231
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3478..6489
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6174..6291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7063..7296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7481..7525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7819..7845
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8130..8149)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8195..8250
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8249..8268)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8611..8627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8611..8627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8624..8646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8635..8651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8659..8689)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8704..8725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8784..8979
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8830..8965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8892..8911
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8985..9119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9022..9041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9076..9095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9187..9204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9358..9946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9438..9457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10117..10977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10740..10759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10978..11082)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg
      541 gggsggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg rgggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgggccgcg
     1741 ttgacgcgca cggcaagagg cgaggggcgg cgactggtga gagatgggtg cgagagcgtc
     1801 agtattaagc gggggagaat tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1861 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1921 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1981 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     2041 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     2101 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca gctgacacag gacacagcaa
     2161 tcaggtcagc caaaattacc ctatagtgca gaacatccag gggcaaatgg tacatcaggc
     2221 catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc
     2281 agaagtgata cccatgtttt cagcattatc agaaggagcc accccacaag atttaaacac
     2341 catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa
     2401 tgaggaagct gcagaatggg atagagtgca tccagtgcat gcagggccta ttgcaccagg
     2461 ccagatgaga gaaccaaggg gaagtgacat agcaggaact actagtaccc ttcaggaaca
     2521 aataggatgg atgacacata atccacctat cccagtagga gaaatctata aaagatggat
     2581 aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc tggacataag
     2641 acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa ctctaagagc
     2701 cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg tccaaaatgc
     2761 gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac tagaagaaat
     2821 gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt tggctgaagc
     2881 aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt ttaggaacca
     2941 aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca aaaattgcag
     3001 ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg
     3061 ttccggtgga ggtggatccg gtggaggttc catggcgtcc aatttcacgc agttcgtcct
     3121 ggttgacaac ggggggactg gggacgttac ggtcgctccg agcaactttg ccaatggtat
     3181 tgcggagtgg atttcttcta attcacggtc ccaagcttac aaagtgacct gttccgtgcg
     3241 gcaaagttct gctcagaata gaaagtacac tataaaggtc gaagtcccta agggggcctg
     3301 gcgatcatat ctcaatatgg agcttaccat cccaatattt gccactaatt ctgattgtga
     3361 attgattgtc aaagcaatgc aaggactctt gaaagacgga aacccaatcc ccagcgcaat
     3421 cgcagccaac tccggtatat actccggagg tggaggtgga actgagagac aggctaattt
     3481 tttagggaag atctggcctt cccacaaggg aaggccaggg aattttcttc agagcagacc
     3541 agagccaaca gccccaccag aagagagctt caggtttggg gaagagacaa caactccctc
     3601 tcagaagcag gagccgatag acaaggaact gtatccttta gcttccctca gatcactctt
     3661 tggcagcgac ccctcgtcac aataaagata ggggggcaat taaaggaagc tctattagat
     3721 acaggagcag atgatacagt attagaagaa atgaatttgc caggaagatg gaaaccaaaa
     3781 atgatagggg gaattggagg ttttatcaaa gtaagacagt atgatcagat actcatagaa
     3841 atctgcggac ataaagctat aggtacagta ttagtaggac ctacacctgt caacataatt
     3901 ggaagaaatc tgttgactca gattggctgc actttaaatt ttcccattag tcctattgag
     3961 actgtaccag taaaattaaa gccaggaatg gatggcccaa aagttaaaca atggccattg
     4021 acagaagaaa aaataaaagc attagtagaa atttgtacag aaatggaaaa ggaaggaaaa
     4081 atttcaaaaa ttgggcctga aaatccatac aatactccag tatttgccat aaagaaaaaa
     4141 gacagtacta aatggagaaa attagtagat ttcagagaac ttaataagag aactcaagat
     4201 ttctgggaag ttcaattagg aataccacat cctgcagggt taaaacagaa aaaatcagta
     4261 acagtactgg atgtgggcga tgcatatttt tcagttccct tagataaaga cttcaggaag
     4321 tatactgcat ttaccatacc tagtataaac aatgagacac cagggattag atatcagtac
     4381 aatgtgcttc cacagggatg gaaaggatca ccagcaatat tccagtgtag catgacaaaa
     4441 atcttagagc cttttagaaa acaaaatcca gacatagtca tctatcaata catggatgat
     4501 ttgtatgtag gatctgactt agaaataggg cagcatagaa caaaaataga ggaactgaga
     4561 caacatctgt tgaggtgggg atttaccaca ccagacaaaa aacatcagaa agaacctcca
     4621 ttcctttgga tgggttatga actccatcct gataaatgga cagtacagcc tatagtgctg
     4681 ccagaaaagg acagctggac tgtcaatgac atacagaaat tagtgggaaa attgaattgg
     4741 gcaagtcaga tttatgcagg gattaaagta aggcaattat gtaaacttct taggggaacc
     4801 aaagcactaa cagaagtagt accactaaca gaagaagcag agctagaact ggcagaaaac
     4861 agggagattc taaaagaacc ggtacatgga gtgtattatg acccatcaaa agacttaata
     4921 gcagaaatac agaagcaggg gcaaggccaa tggacatatc aaatttatca agagccattt
     4981 aaaaatctga aaacaggaaa gtatgcaaga atgaagggtg cccacactaa tgatgtgaaa
     5041 caattaacag aggcagtaca aaaaatagcc acagaaagca tagtaatatg gggaaagact
     5101 cctaaattta aattacccat acaaaaggaa acatgggaag catggtggac agagtattgg
     5161 caagccacct ggattcctga gtgggagttt gtcaataccc ctcccttagt gaagttatgg
     5221 taccagttag agaaagaacc cataatagga gcagaaactt tctatgtaga tggggcagcc
     5281 aatagggaaa ctaaattagg aaaagcagga tatgtaactg acagaggaag acaaaaagtt
     5341 gtccccctaa cggacacaac aaatcagaag actgagttac aagcaattca tctagctttg
     5401 caggattcgg gattagaagt aaacatagtg acagactcac aatatgcatt gggaatcatt
     5461 caagcacaac cagataagag tgaatcagag ttagtcagtc aaataataga gcagttaata
     5521 aaaaaggaaa aagtctacct ggcatgggta ccagcacaca aaggaattgg aggaaatgaa
     5581 caagtagata aattggtcag tgctggaatc aggaaagtac tatttttaga tggaatagat
     5641 aaggcccaag aagaacatga gaaatatcac agtaattgga gagcaatggc tagtgatttt
     5701 aacctaccac ctgtagtagc aaaagaaata gtagccagct gtgataaatg tcagctaaaa
     5761 ggggaagcca tgcatggaca agtagactgt agcccaggaa tatggcagct agtttgtaca
     5821 catttagaag gaaaagttat cttggtagca gttcatgtag ccagtggata tatagaagca
     5881 gaagtaattc cagcagagac agggcaagaa acagcatact tcctcttaaa attagcagga
     5941 agatggccag taaaaacagt acatacagac aatggcagca atttcaccag tactacagtt
     6001 aaggccgcct gttggtgggc ggggatcaag caggaatttg gcattcccta caatccccaa
     6061 agtcaaggag taatagaatc tatgaataaa gaattaaaga aaattatagg acaggtaaga
     6121 gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca caattttaaa
     6181 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     6241 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     6301 tacagggaca gcagagatcc agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa
     6361 ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag aaaagcaaag
     6421 atcatcaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag tagacaggat
     6481 gaggattaac acatggaatt ctgcaacaac tgctgtttat ccatttcaga attgggtgtc
     6541 gacatagcag aataggcgtt actcgacaga ggagagcaag aaatggagcc agtagatcct
     6601 agactagagc cctggaagca tccaggaagt cagcctaaaa ctgcttgtac caattgctat
     6661 tgtaaaaagt gttgctttca ttgccaagtt tgtttcatga caaaagcctt aggcatctcc
     6721 tatggcagga agaagcggag acagcgacga agagctcatc agaacagtca gactcatcaa
     6781 gcttctctat caaagcagta agtagtacat gtaatgcaac ctataatagt agcaatagta
     6841 gcattagtag tagcaataat aatagcaata gttgtgtggt ccatagtaat catagaatat
     6901 aggaaaatgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     7741 cagagacaga tccattcgat tagtgaacgg atccttggca cttatctggg acgatctgcg
     7801 gagcctgtgc ctcttcagct accaccgctt gagagactta ctcttgattg taacgaggat
     7861 tgtggaactt ctgggacgca gggggtggga agccctcaaa tattggtgga atctcctaca
     7921 atattggagt caggagctaa agaatagtgc tgttagcttg ctcaatgcca cagccatagc
     7981 agtagctgag gggacagata gggttataga agtagtacaa ggagcttgta gagctattcg
     8041 ccacatacct agaagaataa gacagggctt ggaaaggatt ttgctataag ctcgaaacaa
     8101 ccggtacctc tagaactata gctagcagat ctttttccct ctgccaaaaa ttatggggac
     8161 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8221 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8281 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     8341 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     8401 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     8461 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     8521 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     8581 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8641 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8701 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8761 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     8821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     9001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     9121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11101 gccacctg
//