c-myc-PT3EF1a

LOCUS       c-myc-PT3EF1a                6494 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses c-Myc in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    c-myc-PT3EF1a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Liu P, Ge M, Hu J, Li X, Che L, Sun K, Cheng L, Huang Y, Pilo MG, 
            Cigliano A, Pes GM, Pascale RM, Brozzetti S, Vidili G, Porcu A, 
            Cossu A, Palmieri G, Sini MC, Ribback S, Dombrowski F, Tao J, 
            Calvisi DF, Chen L, Chen X
  TITLE     A functional mTORC1 signaling is indispensable for c-Myc driven 
            hepatocarcinogenesis.
  JOURNAL   Hepatology. 2017 Mar 30. doi: 10.1002/hep.29183.
  PUBMED    28370287
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1210..1234
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1268..2584
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     protein_bind    complement(2601..2625)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2653..2672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2707..2724)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2713..2937
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3355..3371)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3355..3371)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3368..3390)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3379..3395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3403..3433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3448..3469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3586..3603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3837..3856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4516..5376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5139..5158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5377..5481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5549..5567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5605..5627)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5727..5746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5940..5962
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5954..5971
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5955..5971
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6353..6373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtaccaata
     1201 cggatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
     1261 atccaccatg cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc
     1321 ggtgcagccg tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag
     1381 cgagctgcag cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac
     1441 cccgcccctg tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac
     1501 acccttctcc cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca
     1561 gctggagatg gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga
     1621 cccggacgac gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt
     1681 ctcggccgcc gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga
     1741 cagcggcagc ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct
     1801 gcaggatctg agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc
     1861 tctcaacgac agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc
     1921 gtcctcggat tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct
     1981 ggtgctccat gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga
     2041 tgaggaagaa atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga
     2101 gtctggatca ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa
     2161 gaggtgccac gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga
     2221 ctatcctgct gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa
     2281 caaccgaaaa tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac
     2341 acacaacgtc ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg
     2401 tgaccagatc ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa
     2461 agccacagca tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga
     2521 cttgttgcgg aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg
     2581 tgcggactcg agatatctag acccagcttt cttgtacaaa gtggttgata tcgcggccgc
     2641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     2701 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2761 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2821 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2881 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2941 ctgaggcggg atccccttga aatacatcca caggtacacc tccaattgac tcaaatgatg
     3001 tcaattagtc tatcagaagc ttctaaagcc atgacatcat tttctggaat tttccaagct
     3061 gtttaaaggc acagtcaact tagtgtatgt aaacttctga cccactggaa ttgtgataca
     3121 gtgaattata agtgaaataa tctgtctgta aacaattgtt ggaaaaatga cttgtgtcat
     3181 gcacaaagta gatgtcctaa ctgacttgcc aaaactattg tttgttaaca agaaatttgt
     3241 ggagtagttg aaaaacgagt tttaatgact ccaacttaag tgtatgtaaa cttccgactt
     3301 caactgtata ggtctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
     3361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5701 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     5821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5941 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc tacagttgaa
     6001 gtcggaagtt tacatacact taagttggag tcattaaaac tcgtttttca actactccac
     6061 aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta ctttgtgcat
     6121 gacacaagtc atttttccaa caattgttta cagacagatt atttcactta taattcactg
     6181 tatcacaatt ccagtgggtc agaagtttac atacactaag ttgactgtgc ctttaaacag
     6241 cttggaaaat tccagaaaat gatgtcatgg ctttagaagc ttctgataga ctaattgaca
     6301 tcatttgagt caattggagg tgtacctgtg gatgtatttc aaggaattct gtggaatgtg
     6361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6421 catatcgata ctagtttaat taagatcctc tttgcagcta atggaccttc taggtcttga
     6481 aaggagtggg aatt
//