pCDH-MTS-GFP-LplJ

LOCUS       pCDH-MTS-GFP-LplJ            8265 bp DNA     circular SYN 09-MAY-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8265
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2507..2599
                     /label=MTS
     CDS             2600..3313
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3344..4336
                     /label=LplJ
     misc_feature    4346..4933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4817..4828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5006..5239
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5311..5432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5472..5607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5640..5656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5664..5680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5688..5718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5733..5754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6042..6630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6801..7661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7662..7766)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8240..8256
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatgctgt ttaatctgag
     2521 gatcctgtta aacaatgcag cttttagaaa tggtcacaac ttcatggttc gaaattttcg
     2581 gtgtggacaa ccactacaag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2641 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2701 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2761 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2821 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2881 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2941 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3001 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3061 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3181 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3301 cgagctgtac aagtccggac tcagatctcg agctcaagct tccatgttat ttatagacaa
     3361 tcaaaatatc aatgatccgc ggatcaatct tgctatcgag gagtactgtg taaagcattt
     3421 agaccctgaa cagcaatatt tgctctttta tgtgaatcaa ccgtctatta tcattgggaa
     3481 aaaccaaaat acaatagaag aaatcaatac aaaatatgtt gaggaaaacg ggattatcgt
     3541 cgtccgccgt ttatcaggcg ggggcgctgt gtaccatgat ctggggaact tgaacttcag
     3601 ctttattacc aaggatgacg gggacagctt tcataacttt aaaaaattca ctgagccagt
     3661 gattcaggcg ttacatcagc ttggggttga agccgaatta agcggccgga acgacattgt
     3721 agtagatggc cggaaaatat ccggaaacgc tcagtttgca acgaaaggcc gtattttcag
     3781 ccacggcacc ctcatgtttg attcagccat tgatcatgtt gtatcagcat taaaggtgaa
     3841 aaaggataaa attgaatcaa aaggcatcaa atcgatcaga agccgagtgg caaacatcag
     3901 tgagtttctc gatgataaaa tgaccaccga agaattccgc agccatttgc ttcgccatat
     3961 ttttaacaca aacgacgttg gaaacgtgcc ggagtataag ctgacggaaa aagattggga
     4021 gaccattcat caaatttcga aagagcgcta tcagaattgg gattggaact acggccgctc
     4081 accaaaattt aaccttaatc attcgaagcg ttatccggtt ggatcgatcg atttgcacct
     4141 ggaagtcaag aaaggcaaaa tcgaggactg caaaatcttc ggagatttct tcggtgtcgg
     4201 tgatgtatct gaaattgaaa acctgctggt cggaaaacaa tacgaacgca gcgtcatcgc
     4261 tgatgtgctc gaaggtgtga atctcaagca ttactttggg aacattacga aagaggattt
     4321 ccttgatctg atttactgag gatccaatca acctctggat tacaaaattt gtgaaagatt
     4381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4741 atcatcgtcc tttccttggc tgctcgcctg tgttgcaacc tggattctgc gcgggacgtc
     4801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4861 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     4921 gccgcctccc cgccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4981 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat
     5041 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5101 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5161 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5221 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     5281 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     5341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     5461 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5521 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5581 agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg
     5641 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5701 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5761 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5821 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5881 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5941 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6001 caaaaggcca ggaaccttaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6061 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6481 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7801 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     7861 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7921 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7981 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     8041 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     8101 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     8161 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8221 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//