pCDH_cgeMTS_GFP_LplA

LOCUS       pCDH_cgeMTS_GFP_LplA              8265 bp DNA     circular SYN 03-JUL-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    223..810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(694..705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             883..1116
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1188..1309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1349..1484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1517..1533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1541..1557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1565..1595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1610..1631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1919..2507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2678..3538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3539..3643)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4117..4133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4148..4374
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4375..4555
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4602..4727
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5220..5453
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5638..5682
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5949..6065
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6088..6391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6392..6595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6646..6717
                     /label=cgeSOD2
     CDS             6718..7431
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7462..8265,1..216)
                     /label=LplA
ORIGIN
        1 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga cagcctcaac
       61 cccgcgccgc tggaagccct cgccggacga ctgcaaggct gcctgtaccg cgcagatatg
      121 ctgcaacagg agtgcgaagc gctgttggtt gacttcccgg aacaggaaaa agagctacgg
      181 gagttatcgg catggatggc gggggctgta aggtagggat ccaatcaacc tctggattac
      241 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      301 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      361 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      421 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
      481 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
      541 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
      601 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
      661 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
      721 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
      781 agtcggatct ccctttggcc gcctccccgc cggtaccttt aagaccaatg acttacaagg
      841 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
      901 cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
      961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1081 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1141 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     1201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     1321 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1381 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1441 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga
     1501 ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1561 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1621 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1681 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1741 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1801 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1861 tgtgagcaaa aggccagcaa aaggccagga accttaaaaa ggccgcgttg ctggcgtttt
     1921 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1981 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2041 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2101 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2161 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2221 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2281 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2341 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2401 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2461 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2521 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2581 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2641 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2701 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2761 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2821 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2881 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2941 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3001 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3061 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3121 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3181 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3241 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3301 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3361 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3421 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3481 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3541 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3601 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3661 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3721 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3781 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3841 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     3901 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3961 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4021 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4081 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     4141 tgacgcgtgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     4201 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     4261 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     4321 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     4381 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4441 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4501 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     4561 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     4621 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     4681 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     4741 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     4801 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     4861 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     4921 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     4981 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     5041 aaagtaagac caccgcacag caagcggcca ctgatcttca gacctggagg aggagatatg
     5101 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     5161 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     5221 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5281 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5341 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5401 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5461 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     5521 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     5581 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     5641 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     5701 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     5761 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     5821 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     5881 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     5941 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     6001 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     6061 attttatcga tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     6121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     6181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     6241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     6301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     6361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     6421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     6481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     6541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     6601 agtgaaccgt cagattctag accgctagcg ctaccggtcg ccaccatgct atgtcgtgct
     6661 gcctgctccg ctggccgccg actcgctccc gctgccactg ccgctggatc ccggcatgtg
     6721 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     6781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     6841 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     6901 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     6961 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     7021 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     7081 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     7141 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     7201 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     7261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     7321 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     7381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     7441 agatctcgag ctcaagcttc catgggctcc acattacgcc tgctcatctc tgactcttac
     7501 gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag
     7561 cgcgttctgt ttctctggcg caatgccgac acggtagtaa ttggtcgcgc gcagaacccg
     7621 tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt
     7681 agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc
     7741 aagccggagt acgataaaac tatctccacg tcgattgtgc tcaatgcgct gaacgcgctc
     7801 ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaaccgt cgaaggcgac
     7861 cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gcggcttcca ccacggcacc
     7921 ttgctactca atgccgacct cagccgcctg gcaaactatc tcaatccgga taaaaagaaa
     7981 ctggcggcga aaggcattac gtcggtacgt tcccgcgtga ccaacctcac cgagctgttg
     8041 ccggggatca cccatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
     8101 ggcgagcgcg tggaagcgga aatcatctcc ccgaacaaaa cgccagactt gccaaacttc
     8161 gccgaaacct ttgcccgcca gagtagctgg gaatggaact tcggtcaggc tccggcattc
     8221 tcgcatctgc tggatgaacg ctttacctgg ggcggcgtgg aactg
//