| 质粒名称: | PABE-2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115623 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | wtTadA-TadA7.10-nCas9-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PABE-2 was a gift from Caixia Gao (Addgene plasmid # 115623 ; http://n2t.net/addgene:115623 ; RRID:Addgene_115623) |
| 参考文献部分: | Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545 |
买家导航
PABE-2载体 PABE2质粒基本信息
PABE-2质粒图谱 PABE2载体图谱和PABE-2载体序列 PABE2质粒序列多克隆位点信息
PABE-2质粒 PABE2载体简介
PABE-2质粒序列 PABE2载体序列
LOCUS PABE-2 10294 bp ds-DNA circular SYN 10-JAN-2019
DEFINITION Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION .
VERSION .
KEYWORDS PABE-2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10294)
AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
TITLE Expanded base editing in rice and wheat using a Cas9-adenosine
deaminase fusion.
JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
PUBMED 29807545
REFERENCE 2 (bases 1 to 10294)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10294
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2041..6141
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 6054..6062
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 6154..6174
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 6232..6408
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(6962..6978)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6962..6978)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6975..6997)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6986..7002
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7010..7040)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7055..7076
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7193..7210)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7364..7952)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7444..7463)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8123..8983)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8746..8765
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8984..9088)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg
61 cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt
121 tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt
181 tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattaa
241 ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca
301 tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat
361 gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg
421 atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa
481 ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta
541 cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt
601 actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac
661 ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg
721 atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt
781 tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaaag
841 cttatgtccg aggtggagtt ctctcacgag tattggatga ggcacgctct tacacttgct
901 aagagagctt gggacgaaag agaagtgcca gttggcgccg ttcttgtgca taataatagg
961 gtgatcggcg agggttggaa tagaccaatt ggaaggcatg atccaacagc tcacgcagag
1021 attatggctc tcagacaagg cggcctcgtt atgcagaact acaggctcat tgacgctaca
1081 ctctacgtga cactcgaacc ttgcgttatg tgcgccggag ctatgattca ttctaggatt
1141 ggcagggtcg tgtttggagc tagggacgct aaaacaggag ccgccggatc tcttatggac
1201 gtgttgcatc atccaggcat gaaccatagg gtggagatta cagagggcat tcttgcagac
1261 gagtgcgctg ctcttctttc cgatttcttc aggatgagaa ggcaggagat taaggcccag
1321 aagaaggctc agtcttctac agatagcgga ggatcttccg gaggatctag cggctccgag
1381 acaccaggaa catccgaaag cgctacacca gaatctagcg gaggctcttc cggaggatct
1441 tctgaagtgg agttctccca cgagtattgg atgaggcacg ctcttacact tgctaaaagg
1501 gctagggacg aaagggaagt tccagttgga gctgttctcg tgctcaataa cagggtgatt
1561 ggcgagggtt ggaatagagc cattggactc catgatccaa cagctcacgc agagattatg
1621 gctcttagac aaggcggcct cgttatgcag aattacagac tcatcgacgc cacactctac
1681 gttaccttcg aaccttgcgt tatgtgcgcc ggagctatga tccattctag gattggcagg
1741 gtcgtgttcg gcgttagaaa cgctaagaca ggagctgcag gctctcttat ggacgttctt
1801 cattacccag gcatgaatca tagagtggag atcacagaag gcattcttgc agacgagtgc
1861 gcagctctcc tttgctattt cttcaggatg ccgaggcaag ttttcaacgc tcagaagaag
1921 gcccagtctt ctacagattc cggcggatct tctggaggat ctagcggctc cgagacacca
1981 ggaacatccg aatccgctac accagagtct tctggaggat ctagcggagg atctcttaag
2041 gacaagaagt actcgatcgg cctcgccatt gggactaact ctgttggctg ggccgtgatc
2101 accgacgagt acaaggtgcc ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat
2161 tccatcaaga agaatctcat tggcgctctc ctgttcgaca gcggcgagac ggctgaggct
2221 acgcggctca agcgcaccgc ccgcaggcgg tacacgcgca ggaagaatcg catctgctac
2281 ctgcaggaga ttttctccaa cgagatggcg aaggttgacg attctttctt ccacaggctg
2341 gaggagtcat tcctcgtgga ggaggataag aagcacgagc ggcatccaat cttcggcaac
2401 attgtcgacg aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag
2461 ctcgtggact ccacagataa ggcggacctc cgcctgatct acctcgctct ggcccacatg
2521 attaagttca ggggccattt cctgatcgag ggggatctca acccggacaa tagcgatgtt
2581 gacaagctgt tcatccagct cgtgcagacg tacaaccagc tcttcgagga gaaccccatt
2641 aatgcgtcag gcgtcgacgc gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc
2701 ctcgagaacc tgatcgccca gctgccgggc gagaagaaga acggcctgtt cgggaatctc
2761 attgcgctca gcctggggct cacgcccaac ttcaagtcga atttcgatct cgctgaggac
2821 gccaagctgc agctctccaa ggacacatac gacgatgacc tggataacct cctggcccag
2881 atcggcgatc agtacgcgga cctgttcctc gctgccaaga atctgtcgga cgccatcctc
2941 ctgtctgata ttctcagggt gaacaccgag attacgaagg ctccgctctc agcctccatg
3001 atcaagcgct acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag
3061 cagctccccg agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg
3121 tacattgacg gcggggcctc tcaggaggag ttctacaagt tcatcaagcc gattctggag
3181 aagatggacg gcacggagga gctgctggtg aagctcaatc gcgaggacct cctgaggaag
3241 cagcggacat tcgataacgg cagcatccca caccagattc atctcgggga gctgcacgct
3301 atcctgagga ggcaggagga cttctaccct ttcctcaagg ataaccgcga gaagatcgag
3361 aagattctga ctttcaggat cccgtactac gtcggcccac tcgctagggg caactcccgc
3421 ttcgcttgga tgacccgcaa gtcagaggag acgatcacgc cgtggaactt cgaggaggtg
3481 gtcgacaagg gcgctagcgc tcagtcgttc atcgagagga tgacgaattt cgacaagaac
3541 ctgccaaatg agaaggtgct ccctaagcac tcgctcctgt acgagtactt cacagtctac
3601 aacgagctga ctaaggtgaa gtatgtgacc gagggcatga ggaagccggc tttcctgtct
3661 ggggagcaga agaaggccat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt
3721 aagcagctca aggaggacta cttcaagaag attgagtgct tcgattcggt cgagatctct
3781 ggcgttgagg accgcttcaa cgcctccctg gggacctacc acgatctcct gaagatcatt
3841 aaggataagg acttcctgga caacgaggag aatgaggata tcctcgagga cattgtgctg
3901 acactcactc tgttcgagga ccgggagatg atcgaggagc gcctgaagac ttacgcccat
3961 ctcttcgatg acaaggtcat gaagcagctc aagaggagga ggtacaccgg ctgggggagg
4021 ctgagcagga agctcatcaa cggcattcgg gacaagcagt ccgggaagac gatcctcgac
4081 ttcctgaaga gcgatggctt cgcgaaccgc aatttcatgc agctgattca cgatgacagc
4141 ctcacattca aggaggatat ccagaaggct caggtgagcg gccaggggga ctcgctgcac
4201 gagcatatcg cgaacctcgc tggctcgcca gctatcaaga aggggattct gcagaccgtg
4261 aaggttgtgg acgagctggt gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt
4321 gagatggccc gggagaatca gaccacgcag aagggccaga agaactcacg cgagaggatg
4381 aagaggatcg aggagggcat taaggagctg gggtcccaga tcctcaagga gcacccggtg
4441 gagaacacgc agctgcagaa tgagaagctc tacctgtact acctccagaa tggccgcgat
4501 atgtatgtgg accaggagct ggatattaac aggctcagcg attacgacgt cgatcatatc
4561 gttccacagt cattcctgaa ggatgactcc attgacaaca aggtcctcac caggtcggac
4621 aagaaccggg gcaagtctga taatgttcct tcagaggagg tcgttaagaa gatgaagaac
4681 tactggcgcc agctcctgaa tgccaagctg atcacgcagc ggaagttcga taacctcaca
4741 aaggctgaga ggggcgggct ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg
4801 gtcgagacac ggcagatcac taagcacgtt gcgcagattc tcgactcacg gatgaacact
4861 aagtacgatg agaatgacaa gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag
4921 ctcgtctccg acttcaggaa ggatttccag ttctacaagg ttcgggagat caacaattac
4981 caccatgccc atgacgcgta cctgaacgcg gtggtcggca cagctctgat caagaagtac
5041 ccaaagctcg agagcgagtt cgtgtacggg gactacaagg tttacgatgt gaggaagatg
5101 atcgccaagt cggagcagga gattggcaag gctaccgcca agtacttctt ctactctaac
5161 attatgaatt tcttcaagac agagatcact ctggccaatg gcgagatccg gaagcgcccc
5221 ctcatcgaga cgaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg
5281 accgtcagga aggttctctc catgccacaa gtgaatatcg tcaagaagac agaggtccag
5341 actggcgggt tctctaagga gtcaattctg cctaagcgga acagcgacaa gctcatcgcc
5401 cgcaagaagg actgggatcc gaagaagtac ggcgggttcg acagccccac tgtggcctac
5461 tcggtcctgg ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag
5521 gagctgctgg ggatcacgat tatggagcgc tccagcttcg agaagaaccc gatcgatttc
5581 ctggaggcga agggctacaa ggaggtgaag aaggacctga tcattaagct ccccaagtac
5641 tcactcttcg agctggagaa cggcaggaag cggatgctgg cttccgctgg cgagctgcag
5701 aaggggaacg agctggctct gccgtccaag tatgtgaact tcctctacct ggcctcccac
5761 tacgagaagc tcaagggcag ccccgaggac aacgagcaga agcagctgtt cgtcgagcag
5821 cacaagcatt acctcgacga gatcattgag cagatttccg agttctccaa gcgcgtgatc
5881 ctggccgacg cgaatctgga taaggtcctc tccgcgtaca acaagcaccg cgacaagcca
5941 atcagggagc aggctgagaa tatcattcat ctcttcaccc tgacgaacct cggcgcccct
6001 gctgctttca agtacttcga cacaactatc gatcgcaaga ggtacacaag cactaaggag
6061 gtcctggacg cgaccctcat ccaccagtcg attaccggcc tctacgagac gcgcatcgac
6121 ctgtctcagc tcgggggcga ctccggcggc agcccaaaga agaagcggaa ggtgtaggaa
6181 ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
6241 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
6301 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
6361 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc ctaaagtccc
6421 tatagatctt tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg agcccagacg
6481 ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga tgctaaggca
6541 gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt ggacggaagg
6601 aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga agatggaaat
6661 ttgatagagg tacgctacta tacttatact atacgctaag ggaatgcttg tatttatacc
6721 ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc ccccgcttaa
6781 aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat aaaacctcac
6841 caaaatacga aagagttctt aactctaaag ataaaagatc tttcaagatc aaaactagtt
6901 ccctcacacc ggtgacgggg atcgcatgcg atatctcgag atctagcttg gcgtaatcat
6961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
7021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
7081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
7141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
7201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
7261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
7321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
7381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
7441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
7501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
7561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
7621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
7681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
7741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
7801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
7861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
7921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
7981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
8041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
8101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
8161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
8221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
8281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
8341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
8401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
8461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
8521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
8581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
8641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
8701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
8761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
8821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
9001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
9061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgccagtg
9121 ccaagctaat tcgagctcgg tacctgaccc ggtcgtgccc ctctctagag ataatgagca
9181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
9241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
9301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
9361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
9421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
9481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
9541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
9601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
9661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
9721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
9781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg atcgagagtt
9841 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca
9901 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg
9961 gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc
10021 ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga
10081 tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc
10141 ccccccctct ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc
10201 tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc
10261 gtacacggat gcgacctgta cgtcagacac gttc
//
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PABE-2质粒 PABE2载体应用举例
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