pYK703

LOCUS      pYK703              6090 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..928
                     /label=oYK255_AG_NS3a
     primer_bind     881..925
                     /label=oYK36-AG-Link_s-F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     CDS             906
                     /codon_start=1
                     /label=5xGS Linker
                     /translation=""
     primer_bind     907..926
                     /label=oYK41-44 AG-LplA-F
     primer_bind     907..926
                     /label=oYK45-46 AG-lplA-F
     misc_feature    907..909
                     /label=Long_Linker
     misc_feature    910..1584
                     /label=AG
     primer_bind     1358..1368
                     /label=oSCL1104
     primer_bind     complement(1569..1584)
                     /label=oYK37-AG-R
     primer_bind     complement(1570..1607)
                     /label=oYK224_AG_R
     primer_bind     complement(1588..1610)
                     /label=oYK57-MCP-mCherry-R
     terminator      1684..1911
                     /label=bGH PA
     misc_feature    1957..2385
                     /label=f1 origin
     misc_feature    2390..2733
                     /label=SV40 early promoter and origin
     misc_feature    2579..2656
                     /label=SV40 ORI
     CDS             2798..3589
                     /label=Kan/neoR
     misc_feature    3763..3893
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(3929..3949)
                     /label=M13-rev
     misc_binding    complement(3955..3977)
                     /label=LacO
     promoter        complement(3982..4011)
                     /label=lac
     misc_feature    complement(4276..4949)
                     /label=pUC origin
     CDS             complement(5097..5756)
                     /label=AmpR
     promoter        complement(5996..6024)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg tgagcgtgat caagcccgag atgaagatca agctgtgcat gaggggcacc
      961 gtgaacggcc acaacttcgt gatcgagggc gagggcaagg gcaaccccta cgagggcacc
     1021 cagatcctgg acctgaacgt gaccgagggc gcccccctgc ccttcgccta cgacatcctg
     1081 accaccgtgt tccagtacgg caacagggcc ttcaccaagt accccgccga catccaggac
     1141 tacttcaagc agaccttccc cgagggctac cactgggaga ggagcatgac ctacgaggac
     1201 cagggcatct gcaccgccac cagcaacatc agcatgaggg gcgactgctt cttctacgac
     1261 atcaggttcg acggcgtgaa cttccccccc aacggccccg tgatgcagaa gaagaccctg
     1321 aagtgggagc ccagcaccga gaagatgtac gtgagggacg gcgtgctgaa gggcgacgtg
     1381 aacatggccc tgctgctgga gggcggcggc cactacaggt gcgacttcaa gaccacctac
     1441 aaggccaaga aggacgtgag gctgcccgac taccacttcg tggaccacag gatcgagatc
     1501 ctgaagcacg acaaggacta caacaaggtg aagctgtacg agaacgccgt ggccaggtac
     1561 agcatgctgc ccagccaggc caagtaagga tccactagtc cagtgtggtg gaattctgca
     1621 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     1681 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     1741 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     1801 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     1861 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     1921 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     1981 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2041 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2101 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2161 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2221 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2281 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2341 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     2401 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2461 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2521 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2641 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     2701 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     2761 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     2821 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     2881 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     2941 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     3001 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3061 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     3121 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     3181 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     3241 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     3301 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     3361 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     3421 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     3481 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     3541 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     3601 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     3661 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     3721 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     3781 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3841 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     3901 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6061 acatttcccc gaaaagtgcc acctgacgtc
//