| 质粒名称: | pBOB-Lck-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 118738 |
| 存储实验室: | John Cooper |
|
载体骨架基本信息 |
|
| 载体名称: | pCSC-SP-PW-GFP |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 9303bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Lck domain |
| 物种来源: | MutationH8N (see comments) |
| 启动子: | CMV |
| 标签/融合蛋白: | GFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Xba I (unknown if destroyed) |
| 3’克隆位点: | Age I (unknown if destroyed) |
|
文献引用方法 |
|
| 材料和方法部分: | pBOB-Lck-GFP was a gift from John Cooper (Addgene plasmid # 118738 ; http://n2t.net/addgene:118738 ; RRID:Addgene_118738) |
买家导航
pBOB-Lck-GFP载体质粒基本信息
pBOB-Lck-GFP质粒图谱载体图谱和pBOB-Lck-GFP载体序列质粒序列多克隆位点信息
pBOB-Lck-GFP质粒载体简介
pBOB-Lck-GFP质粒序列载体序列
LOCUS pBOB-Lck-GFP 9303 bp DNA circular SYN 14-APR-2025
DEFINITION To express membrane targeted GFP .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9303)
TITLE Cooper Lab plasmids
REFERENCE 2 (bases 1 to 9303)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Sequence Label: pBOB-Lck-GFP
FEATURES Location/Qualifiers
source 1..9303
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 35..151
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 174..477
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 478..681
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 678..702
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 758..767
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 893..902
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(899..901,902..904,905..1618)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(899..901,902..904,905..1615)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(944..965)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1205..1224)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1552..1573
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1754..2342
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1807..1827)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2225..2236)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2749..2929
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(2955..2972)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2961..3185
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3231..3659
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3318..3337)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3528..3549
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3668..3688)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3673..4002
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3853..3988
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3915..3934
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 4050..4097
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 4116..4490
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 4620..4741
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4657..4676)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4711..4730
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4790..4806)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4790..4806)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4803..4825)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4814..4830
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4838..4844,4845..4862,4863..4868))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4883..4904
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5021..5038)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5192..5780)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5272..5291)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5951..6742,6743..6811))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6574..6593
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6812..6916)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6991..7010)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 7182..7561
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7563..7761
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 7779..7959
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8006..8131
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8624..8857
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9042..9086
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
ORIGIN
1 gagacagatc cattcgatta gtgaacggat caacttttaa aagaaaaggg gggattgggg
61 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
121 tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca
181 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
241 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
301 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
361 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
421 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
481 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
541 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
601 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
661 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
721 cgctgttttg acctccatag aagacaccga ctctagagcc accatgggct gtggctgcag
781 ctcaaaccct gaagatgact ggatggagaa cattgacgtg tgtgagaact gccattatcc
841 cggcggacga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
901 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
961 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
1021 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
1081 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
1141 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
1201 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
1261 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
1321 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
1381 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
1441 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
1501 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
1561 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
1621 cggccgcgtc gagtttaaac ctggaaaaac atggagcaat cacaagtagc aatacagcag
1681 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
1741 tcacacctca gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
1801 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
1861 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
1921 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
1981 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
2041 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
2101 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
2161 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
2221 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
2281 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
2341 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
2401 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
2461 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
2521 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
2581 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
2641 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
2701 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
2761 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
2821 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
2881 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggccgcttta
2941 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
3001 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
3061 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
3121 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
3181 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
3241 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3301 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3361 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3421 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
3481 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
3541 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
3601 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
3661 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
3721 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3901 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
3961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
4021 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
4081 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
4141 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
4201 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
4261 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
4321 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
4381 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
4441 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
4501 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4561 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4741 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4921 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
6961 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
7021 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
7081 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
7141 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
7201 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
7261 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
7321 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
7381 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
7441 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
7501 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
7561 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
7621 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
7681 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
7741 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
7801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
7861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
7921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
7981 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
8041 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
8101 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
8161 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
8221 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
8281 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
8341 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
8401 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
8461 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
8521 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
8581 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
8641 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
8701 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
8761 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
8821 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
8881 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
8941 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
9001 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
9061 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
9121 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
9181 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
9241 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
9301 aca
//
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pBOB-Lck-GFP质粒载体应用举例
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