| 质粒名称: | pYK642 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000828 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-L7Ae expression vector (PhCMV-NLS-L7Ae-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | NLS-L7Ae |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK642
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK642载体质粒基本信息
pYK642质粒图谱载体图谱和pYK642载体序列质粒序列多克隆位点信息
pYK642质粒载体简介
pYK642质粒序列载体序列
LOCUS pYK642 5827 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5827)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5827
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..899
/label=oYK323_mcp_F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
CDS 905..964
/codon_start=1
/label=NLS
/translation="MPKKKRKVEASASPKKKRKV"
primer_bind complement(942..964)
/label=oYK636_NLS_R
primer_bind 945..983
/label=oYK641_L7Ae_F
primer_bind 964..985
primer_bind 964..985
/label=DDL7AeF
/note="KpnI"
primer_bind 964..980
/label=oDJ197-1
misc_feature 965..1321
/label=L7Ae
primer_bind 965..985
/label=L7Ae FLP
primer_bind 1285..1297
/label=oyk03-03-F
primer_bind 1285..1297
/label=oYK04-ns3a-F
primer_bind 1285..1295
/label=oYK25-NS3a-F
primer_bind complement(1297..1321)
/label=DDL7AeR
/note="EcoRI"
primer_bind complement(1301..1343)
/label=oYK642_L7Ae_R
primer_bind complement(1301..1330)
/label=L7Ae RLE
primer_bind complement(1325..1347)
/label=oYK57-MCP-mCherry-R
primer_bind 1325..1347
/label=oYK377_Bam_R
primer_bind complement(1325..1347)
/label=oYK57-MCP-mCherry-R
terminator 1421..1648
/label=bGH PA
misc_feature 1694..2122
/label=f1 origin
misc_feature 2127..2470
/label=SV40 early promoter and origin
misc_feature 2316..2393
/label=SV40 ORI
CDS 2535..3326
/label=Kan/neoR
misc_feature 3500..3630
/label=SV40 early polyadenylation signal
primer_bind complement(3666..3686)
/label=M13-rev
misc_binding complement(3692..3714)
/label=LacO
promoter complement(3719..3748)
/label=lac
misc_feature complement(4013..4686)
/label=pUC origin
CDS complement(4834..5493)
/label=AmpR
primer_bind 5150..5161
/label=oyk03-03-F
primer_bind 5150..5161
/label=oYK04-ns3a-F
promoter complement(5733..5761)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatgtac gtgcgcttcg aggtgcccga ggacatgcag aacgaggccc tgagcctgct
1021 ggaaaaagtg cgcgagagcg gcaaagtgaa gaagggcacc aacgaaacca ccaaggccgt
1081 ggaacggggc ctggccaagc tggtgtatat cgccgaggac gtggaccccc ccgagattgt
1141 ggcccatctg cccctgctgt gcgaagagaa gaacgtgccc tacatctacg tgaagtccaa
1201 gaacgacctg ggcagagccg tgggcatcga ggtgccatgt gcctctgccg ccatcatcaa
1261 cgagggcgag ctgcggaaag aactgggcag cctggtggaa aagatcaagg gcctgcagaa
1321 gtaaggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
1381 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
1441 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
1501 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
1561 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
1621 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
1681 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
1741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
1801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
1861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
1921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
1981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
2041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
2101 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
2161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
2281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
2341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
2401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
2461 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
2521 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
2581 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
2641 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
2701 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
2761 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
2821 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
2881 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
2941 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
3001 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
3061 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
3121 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
3181 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
3241 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
3301 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
3361 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
3421 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
3481 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
3541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
3601 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
3661 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
3721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
3781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
3841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
3901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
3961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
4021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
4081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
4141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
4201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
4261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
4321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
4381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
4441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
4501 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
4561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
4621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
4681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
4741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
4801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
4861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
4921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
4981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
5041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
5101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
5161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
5221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
5281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
5341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
5401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
5461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
5521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
5581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
5641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
5701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
5761 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
5821 tgacgtc
//
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