| 质粒名称: | pSL1394 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002435 |
| 产品用途: | SB100X-specific transposon containing a constitutive BlastR expression unit and a constitutive expression unit for codon-optimized LIRP (ITR(SB)-PSV40-BlastR-pA:PhCMV-LIRP-pA-ITR(SB)). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSBbi-BP |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV, PSV40 |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | BlastR, LIRP |
| 物种来源: | synthetic |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1394
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1394载体质粒基本信息
pSL1394质粒图谱载体图谱和pSL1394载体序列质粒序列多克隆位点信息
pSL1394质粒载体简介
pSL1394质粒序列载体序列
LOCUS pSL1394 5996 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5996)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5996
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=oSCL812
misc_feature complement(84..115)
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
misc_feature complement(281..311)
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
promoter 432..536
/label=AmpR promoter
misc_feature 537..1394
/label=AmpR
/label=nonstandard type: resistance
rep_origin 1492..2174
/label=ColE1 origin
misc_feature complement(2344..2375)
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
misc_feature 2541..2569
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
primer_bind complement(2635..2673)
/label=oSCL807
primer_bind 2654..2693
/label=oSCL845
misc_feature complement(2654..2673)
/label=P_RPBSA
/label=nonstandard type: promoter_const
primer_bind 2654..2673
/label=oSCL808
primer_bind complement(2654..2673)
/label=oSCL807a
primer_bind 2672..2688
/label=oSCL771
misc_feature 2688..3275
/label=CMV
primer_bind 3319..3356
/label=oSCL2337
primer_bind 3319..3338
/label=T7
primer_bind complement(3319..3338)
/label=oSCL1121
primer_bind 3338..3347
/label=oSCL818
primer_bind 3339..3361
/label=oSCL2210
primer_bind 3339..3356
/label=Oscl32-1
primer_bind 3339..3356
/label=Oscl42-1
primer_bind 3340..3356
/label=oSCL1118
regulatory 3340..3348
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3348..3500
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
CSVRQS"
primer_bind complement(3409..3454)
/label=oSCL1156
primer_bind 3435..3455
/label=oSCL1157
primer_bind complement(3442..3517)
/label=oSCL2215
misc_feature 3461^3462
/label=Feature 16
primer_bind 3462..3481
/label=oSCL2216
primer_bind 3481..3518
/label=oSCL2328
primer_bind 3481..3518
/label=oSCL2329
primer_bind 3481..3518
/label=oSCL2330
primer_bind complement(3481..3500)
/label=oSCL2327
primer_bind complement(3489..3517)
/label=oSCL2217
primer_bind 3500..3518
/label=oSCL1757
primer_bind complement(3500..3517)
/label=oSCL2219
misc_feature 3501..3911
/label=LOV2
primer_bind complement(3890..3911)
/label=oSCL1758
primer_bind 3892..3938
/label=oSCL2218
primer_bind 3892..3911
/label=oSCL2216
primer_bind 3892..3911
/label=oSCL2220
CDS 3912..4139
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 3912
/label=Feature 17
primer_bind complement(4013..4034)
/label=oSCL2219
primer_bind 4034..4054
/label=oSCL2220
misc_feature 4034^4035
/label=Feature 18
primer_bind complement(4119..4169)
/label=oSCL093
primer_bind complement(4119..4169)
/label=oSCL396
primer_bind complement(4119..4139)
/label=Oscl32-2
primer_bind complement(4119..4139)
/label=oSCL2211
primer_bind 4139..4159
/label=oLZ048 NSP3-F
misc_feature 4140..4175
/label=linker
misc_feature 4140..4170
/label=linker
primer_bind 4140..4169
/label=oSCL388
primer_bind 4140..4169
/label=oSCL388a
primer_bind complement(4140..4159)
/label=oSCL050
primer_bind 4140..4159
/label=oSCL1192
primer_bind 4140..4157
/label=oLZ147 NS3a F
primer_bind 4140..4156
/label=oYF0033
primer_bind 4143..4194
/label=oYF0025
primer_bind complement(4150..4189)
/label=oSCL1119
primer_bind complement(4150..4169)
/label=oSCL075
primer_bind 4153..4211
/label=oSCL1082
primer_bind 4163..4191
/label=Primer 2
primer_bind 4175..4194
/label=oYF009
CDS 4176..4763
/codon_start=1
/label=NS3a
/translation="KKKGSVVIVGRINLSGDTAYAQQTRGEEGCQETSQTGRDKNQVEG
EVQIVSTATQTFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSAGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 4176..4194
/label=oFY001
primer_bind 4176..4194
/label=oYF0013
primer_bind complement(4744..4772)
/label=oLZ148 NS3a R
primer_bind complement(4745..4802)
/label=oYF0014
primer_bind complement(4745..4772)
/label=oYF0026
primer_bind complement(4745..4769)
/label=Primer 1
primer_bind complement(4745..4769)
/label=oSCL768
primer_bind complement(4745..4763)
/label=oFY002
primer_bind complement(4745..4763)
/label=oYF0010
primer_bind 4749..4768
/label=oYF0033
primer_bind 4749..4768
/label=oSCL1192
primer_bind complement(4750..4771)
/label=oYF0035
primer_bind complement(4750..4769)
/label=oSCL766
primer_bind 4750..4768
/label=oLZ147 NS3a F
primer_bind complement(4764..4783)
/label=oSCL1788
primer_bind complement(4764..4783)
/label=oSCL1130
CDS 4765..4769
/codon_start=1
/label=linker
/translation="S"
terminator 4846..5073
/label=bGH PA
primer_bind complement(5055..5096)
/label=oSCL809
primer_bind 5077..5113
/label=oSCL841
misc_feature 5097..5305
/label=pSV40
primer_bind complement(5287..5327)
/label=oSCL844
primer_bind complement(5287..5324)
/label=oSCL842
primer_bind 5308..5362
/label=oSCL813
primer_bind 5308..5348
/label=oSCL818
promoter 5308..5312
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind 5328..5362
/label=oCW11
primer_bind 5338..5362
/label=oSCL517
misc_feature 5349..5741
/label=BlastR
/label=nonstandard type: resistance
primer_bind complement(5408..5446)
/label=oCW13
primer_bind 5427..5458
/label=oCW14
primer_bind 5639..5649
/label=oSCL841
primer_bind complement(5724..5770)
/label=oCW12
primer_bind complement(5724..5770)
/label=oCW15
primer_bind complement(5725..5750)
/label=oSCL516
primer_bind 5751..5793
/label=oSCL815
polyA_signal 5777..5968
/label=SV40 late polyA
primer_bind complement(join(5972..5996,1..20))
/label=oSCL816
ORIGIN
1 cttctgaggc ggaaagaacc agctgggcct actagatagc ggacccctta ccgaaacatc
61 gccgcattct gcagaggagt cgaacttagt gtatgtaaac ttctgaccca ctggaattgt
121 gatacagtga attataagtg aaataatctg tctgtaaaca attgttggaa aaatgacttg
181 tgtcatgcac aaagtagatg tcctaactga cttgccaaaa ctattgtttg ttaacaagaa
241 atttgtggag tagttgaaaa acgagtttta atgactccaa cttaagtgta tgtaaacttc
301 cgacttcaac tgtataggga tccaatttta acaaaatatt aacgcttaca atttcctgat
361 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc
421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
541 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
601 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
661 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
721 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
781 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
841 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
901 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
961 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
1021 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
1081 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
1141 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
1201 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
1261 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
1321 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
1381 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
1441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
1501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
1561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
1621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
1681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
1741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
1801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
1861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
1921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
1981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
2041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
2101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
2161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
2221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
2281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggatccct
2341 atacagttga agtcggaagt ttacatacac cttagccaaa tacatttaaa ctcacttttt
2401 cacaattcct gacatttaat cctagtaaaa attccctgtc ttaggtcagt taggatcacc
2461 actttatttt aagaatgtga aatatcagaa taatagtaga gagaatgatt catttcagct
2521 tttatttctt tcatcacatt cccagtgggt cagaagttta catacactcg actcctctgc
2581 agaatgcggc gatgtttcgg taaggggtcc cgagtagcta gttcatggca gccagcatat
2641 ggcatatgtt gccttttgtg agacagggtc tcgggccaga tatacgcgtt gacattgatt
2701 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
2761 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
2821 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
2881 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
2941 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
3001 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
3061 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
3121 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
3181 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
3241 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
3301 ttactggctt atcgaaatta atacgactca ctataggggc caccatggct tcaaacttta
3361 ctcagttcgt gctcgtggac aatggtggga caggggatgt gacagtggct ccttctaatt
3421 tcgctaatgg gatcgcagag tggatcagct ccaactcacg gagccaggcc tacaaggtga
3481 catgcagcgt caggcagtct ttggaacgta tcgaaaagaa ttttgtcatc acggacccgc
3541 gtctgcccga caatccgatt atcttcgcgt cagactcttt cttacaactg actgagtata
3601 gtagagagga gatattgggg cgtaactgta gatttcttca ggggccagaa actgatcggg
3661 ctaccgttcg caagatacgt gacgcaatag acaaccagac cgaggtgacg gtgcagctga
3721 ttaactacac aaagtctggg aagaagttct ggaacctgtt tcatttacaa cctatgaggg
3781 accaaaaagg tgacgttcaa tatttcatcg gggttcagtt agatgggact gagcacgtga
3841 gagatgcagc agaaagagag ggtgtaatgc ttattaaaaa aacagccgag aatatcgacg
3901 aagccgctgg tcagaagaga aagtatacca tcaaggtgga ggtccccaaa gtggctaccc
3961 agacagtggg cggagtcgaa ctgcctgtcg ccgcttggag gtcctacctg aacatggagc
4021 tcactatccc aattttcgct accaattctg actgtgaact catcgtgaag gcaatgcagg
4081 ggctcctcaa agacggtaat cctatccctt ccgccatcgc cgctaactca ggtatctacg
4141 ccagcggaag tggcggagga ggcgacgtcg gatccaagaa gaagggcagc gtggtgatcg
4201 tgggcagaat caacctgagc ggcgacaccg cctacgccca gcagaccaga ggcgaggagg
4261 gctgccagga gaccagccag accggcagag acaagaacca ggtggagggc gaggtgcaga
4321 tcgtgagcac cgccacccag accttcctgg ccaccagcat caacggcgtg ctgtggaccg
4381 tgtaccacgg cgccggcacc agaaccatcg ccagccccaa gggccccgtg acccagatgt
4441 acacgaacgt ggacaaggac ttagtgggct ggcaggcccc ccaaggcagc agaagcctga
4501 ccccctgcac ctgcggcagc agcgacctgt acctggtgac cagacacgcc gacgtgatcc
4561 ccgtgagaag aagaggcgac agcagaggca gcctgctgag ccccagaccc atcagctatc
4621 tgaaaggctc agctggcggc cccctgttgt gtcccgccgg ccatgccgtg ggcatcttca
4681 gagccgccgt gagcaccaga ggcgtggcca aggccgtgga cttcatcccc gtggagagcc
4741 tggagaccac catgagaagc cccgctagct agaaattctg cagatatcca gcacagtggc
4801 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
4861 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
4921 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
4981 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
5041 gcaggcatgc tggggatgcg gtgggctcta tggactaagg tcacagcttg tctgtcgatc
5101 tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
5161 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
5221 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
5281 tttggaggcc taggcttttg caaaaagttt cgacctgcag cccagctagg aatagctagg
5341 ccaccatggc catgcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
5401 caatcaacag catccccatc tctgaggact acagcgtcgc cagcgcagct ctctctagcg
5461 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
5521 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
5581 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
5641 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
5701 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaacagctt aattcactcc
5761 tcaggtgcag tcgcgtcaga catgataaga tacattgatg agtttggaca aaccacaact
5821 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
5881 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
5941 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtgg
//
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