phiLOV3-N1

LOCUS       phiLOV3-N1              4346 bp DNA     circular SYN 01-JUN-2023
DEFINITION  Vector for fusing EGFP to the C-terminus of a partner protein. For 
            other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION   U55762
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4346
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     489..512
                     /label=CMV-F
     misc_feature    591..671
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     misc_feature    673..678
                     /label=Kozak
     CDS             679..1008
                     /codon_start=1
                     /label=phiLOV3
                     /translation="MEKSFVITDPRLPDYPIIFASDGFLELTEYSREEIMGRNARFLQG
                     PETDQATVQKIRDAIRDRRETTVQLINYTKSGKKFWNLLHLQPVRDGKGGLQYFIGVQL
                     VGSDHV"
     polyA_signal    1134..1255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1210..1235)
                     /label=SV40-R
     rep_origin      complement(1262..1717)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1744..1848
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1850..2207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     2048..2057
                     /label=CMV-F
     rep_origin      2058..2193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2069..2078
                     /label=CMV-F
     CDS             2242..3036
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3268..3315
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      3644..4232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcgccaccat ggagaagtcc tttgtgatta cagacccacg gctgcccgac
      721 taccctatta ttttcgctag tgacggattc ctggagctga ccgagtactc tcgggaggaa
      781 atcatgggaa ggaacgcacg cttcctgcag ggacccgaga ctgaccaggc caccgtgcag
      841 aagatccggg acgctattcg agatcggaga gaaaccacag tccagctgat caactacaca
      901 aagagcggca agaaattctg gaatctgctg cacctgcagc ctgtgcggga cggaaaaggc
      961 gggctgcagt attttattgg agtgcagctg gtcggctccg atcatgtcta aagcggccgc
     1021 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1081 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1201 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     1261 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     1321 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     1381 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     1441 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     1501 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     1561 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     1621 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     1681 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     1741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     1861 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     1921 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1981 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     2041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     2101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     2161 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     2221 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     2281 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2341 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2401 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     2461 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2521 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     2581 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2641 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2701 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2761 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     2821 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     2881 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     2941 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3001 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     3061 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     3121 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     3181 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     3241 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     3301 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     3361 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     3421 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     3481 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4321 tggataaccg tattaccgcc atgcat
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