| 质粒名称: | pAAV-CAG-NES-NCaMP7 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000159 |
| 产品用途: | Expresses the NCaMP7 brightest green calcium indicator in the cytosol of mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | NCaMP7 |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein
Subach O.M., Sotskov V.P., Plusnin V.V., Gruzdeva A.M., Barykina N.V., Ivashkina O.I., Anokhin K.V., Nikolaeva A.Y., Korzhenevskiy D.A., Vlaskina A.V., Lazarenko V.A., Boyko K.M., Rakitina T.V., Varizhuk A.M., Pozmogova G.E., Podgorny O.V., Piatkevich K.D., Boyden E.S., Subach F.V.
Int. J. Mol. Sci. 2020, Vol. 21, Page 1644 21, 1644 (2020)
|
买家导航
pAAV-CAG-NES-NCaMP7载体质粒基本信息
pAAV-CAG-NES-NCaMP7质粒图谱载体图谱和pAAV-CAG-NES-NCaMP7载体序列质粒序列多克隆位点信息
pAAV-CAG-NES-NCaMP7质粒载体简介
pAAV-CAG-NES-NCaMP7质粒序列载体序列
LOCUS pAAV-CAG-NES-NCaMP7 6064 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6064)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6064
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3652..3678
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3688..4941
/codon_start=1
/label=NCaMP7
/translation="MVSKGEEENMASLPATHELHIFGSINGIDFDMVGQGTGNPNDGYE
ELNLKSTMGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
GASLTVNYRYTYEGSHIKGEAQVEGTGFPADGPVMTNSLTAEAHDQLTEEQIAEFKEAF
SLFDKDGDGTITTKELGTVMRSLGQNPTEAELRVMIIEVDADGDGTLDFPEFLAMMARK
MKYRDTEEEIREAFGVFDKDGNGYIGAAELRHVMTNLGEKLTDEEVGELIREADIDGDG
QVNYEEFVQMMTAKGGSGGGSSSRRKWNKAGHAVRAIGRLSSMYFADWCVSKKTCPNDK
TIVSTFKWAFITDNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNF
KEWQKAFTDVMGMDELYK"
CDS 4600..4656
/codon_start=1
/product="calmodulin-binding peptide from avian smooth
muscle myosin light chain kinase"
/label=calmodulin-binding peptide
/translation="RRKWNKAGHAVRAIGRLSS"
misc_feature 4966..5554
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5437..5448)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5597..5718
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccgaca ccatgcttca acttcctcct
3661 cttgaacgtc ttactctttc gagatctatg gtctcaaagg gagaggagga aaacatggct
3721 agtctgcctg ctacccacga actgcatatt tttggctcca taaatggcat cgatttcgac
3781 atggtaggcc aaggcaccgg taatccaaac gacggctatg aggagctgaa cctgaagtcc
3841 accatgggcg atctgcagtt cagtccctgg atcctggtgc cgcacattgg gtacgggttt
3901 caccagtatc ttccctatcc agacgggatg tcaccatttc aggctgcaat ggtcgacggg
3961 tccggatacc aagtgcaccg cactatgcag tttgaggacg gtgcctcact gaccgtgaat
4021 tacagataca cctacgaagg atcacatatc aaaggggaag ctcaggtgga gggcactggg
4081 ttcccagctg acggccctgt gatgacaaat tccctgacag ccgaggccca cgaccagctg
4141 actgaagagc agatcgctga atttaaagag gctttctccc tatttgacaa ggacggggat
4201 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
4261 gaagcagagc tgcgggtcat gatcattgaa gtagatgccg acggtgacgg cacactcgac
4321 ttccctgagt tcctggcaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
4381 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcgg tgcagcagag
4441 cttcgccatg tgatgacaaa ccttggagag aagttaacag atgaggaggt tggtgaattg
4501 atcagggaag cagacatcga tggggatggt caggtgaact acgaggagtt tgtacaaatg
4561 atgacagcga agggcggttc aggaggcggg agctcatcac gtcgtaagtg gaataaggca
4621 ggtcacgcag tcagagccat aggtcggctg agctcaatgt acttcgctga ctggtgcgta
4681 tccaagaaga cttgccccaa tgataagact atcgtctcca catttaagtg ggcttttatc
4741 accgacaacg gcaaacgcta ccggtctacc gctcgcacca catacacatt cgccaagcca
4801 atggccgcaa attacctcaa aaatcagccg atgtatgtct ttcggaaaac agagctgaaa
4861 cattctaaga ccgaactgaa ttttaaagag tggcaaaagg cctttacgga cgtgatgggc
4921 atggacgagc tgtataagta ggaattcgat atcaagctta tcgataatca acctctggat
4981 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
5041 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
5101 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
5161 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
5221 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
5281 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
5341 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
5401 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
5461 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
5521 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc cgggcggccg
5581 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
5641 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
5701 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
5761 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
5821 gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa
5881 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
5941 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
6001 agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
6061 cgct
//
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